Click here to switch to the map view.

The map label for this gene is ygjG

Identifier: 218701846

GI number: 218701846

Start: 3726649

End: 3728055

Strand: Direct

Name: ygjG

Synonym: ECIAI39_3571

Alternate gene names: 218701846

Gene position: 3726649-3728055 (Clockwise)

Preceding gene: 218701844

Following gene: 218701848

Centisome position: 72.61

GC content: 54.94

Gene sequence:

>1407_bases
GTGATACGCGAGCCTCCGGAGCATATTTTGAACAGGTTACCTTCGAGCGCATCGGCTTTAGCGTGCAGCGCCCACGCCCT
GAATCTCATTGAAAAGCGAACGCTGGATCATGAGGAGATGAAAGCACTTAACCGAGAGGTGATTGAATACTTCAAAGAGC
ATGTCAATCCGGGGTTTTTAGAGTATCGCAAATCTGTTACCGCCGGCGGGGATTACGGAGCCGTAGAGTGGCAAGCGGGA
AGTTTAAATACGCTTGTCGACACCCAGGGACAGGAGTTTATCGACTGCCTGGGAGGTTTTGGAATTTTCAACGTGGGGCA
CCGTAATCCAGTTGTGGTTTCCGCCGTACAGAATCAACTTGCGAAACAACCACTGCACAGCCAGGAGCTACTCGATCCGT
TACGGGCTATGCTGGCGAAAACCCTTGCTGCGCTAACGCCAGGTAAACTGAAATACAGCTTCTTCTGTAATAGCGGCACC
GAGTCCGTTGAAGCGGCGCTGAAGCTGGCGAAGGCTTATCAGTCGCCGCGCGGCAAATTTACTTTTATTGCCACCAGCGG
CGCGTTCCACGGTAAATCACTTGGCGCGCTGTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCACTGCTGC
CAGGGTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGAT
GTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACG
TAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCATGGGGCGCACGGGCAAGATGTTCG
CCTGCGAGCATGAGAACGTACAGCCGGATATCCTCTGCCTTGCCAAAGCGCTCGGCGGCGGCGTGATGCCGATTGGCGCG
ACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCT
GACCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAACAAAAAGGCGATA
TGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTGCAGGAAGCACGTGGTAAAGGGATGTTGATG
GCGATTGAGTTTGTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAAC
GCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGG
CGCGTAAGGCGCTGGCGGCCATGCGAGTAAGTGTCGAAGAAGCGTAG

Upstream 100 bases:

>100_bases
ATTAAATATTGCACAAATGATAACCGAATTTGTGTTTATCCCGATTTTCGCGATCGCAGCCGGAGTGGCGCAATCCCTGC
AATACTTAAATCGGTATCAT

Downstream 100 bases:

>100_bases
TATCAAATCGGATGGCGATACGACGTCGCCATCCGATCTTTTTATACGATACGTACGCCCGCAGGCATCATCCGCTCCGG
CGTTAACAGCACGCTTTCAC

Product: putrescine--2-oxoglutarate aminotransferase

Products: NA

Alternate protein names: Putrescine--2-oxoglutaric acid transaminase; PAT; PATase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MIREPPEHILNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAG
SLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGT
ESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDD
VAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGA
TIATEEVFSVLFDNPFLHTTTFGGNPLTCAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLM
AIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA

Sequences:

>Translated_468_residues
MIREPPEHILNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAG
SLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGT
ESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDD
VAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGA
TIATEEVFSVLFDNPFLHTTTFGGNPLTCAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLM
AIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA
>Mature_468_residues
MIREPPEHILNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGAVEWQAG
SLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGT
ESVEAALKLAKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDD
VAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILCLAKALGGGVMPIGA
TIATEEVFSVLFDNPFLHTTTFGGNPLTCAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLM
AIEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA

Specific function: Catalyzes the aminotransferase reaction from putrescine to 2-oxoglutarate, leading to glutamate and 4-aminobutanal, which spontaneously cyclizes to form 1-pyrroline. Is also able to transaminate cadaverine and, in lower extent, spermidine, but not ornithi

COG id: COG4992

COG function: function code E; Ornithine/acetylornithine aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. Putrescine aminotransferase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=384, Percent_Identity=33.59375, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI284507298, Length=312, Percent_Identity=35.8974358974359, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI13994255, Length=460, Percent_Identity=30.4347826086957, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI37574042, Length=405, Percent_Identity=27.6543209876543, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI226442705, Length=405, Percent_Identity=27.4074074074074, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI226442709, Length=385, Percent_Identity=27.7922077922078, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI24119277, Length=408, Percent_Identity=26.7156862745098, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI188536080, Length=433, Percent_Identity=23.7875288683603, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI38679950, Length=433, Percent_Identity=23.7875288683603, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI38679946, Length=433, Percent_Identity=23.7875288683603, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI145693181, Length=459, Percent_Identity=99.5642701525055, Blast_Score=939, Evalue=0.0,
Organism=Escherichia coli, GI1789759, Length=330, Percent_Identity=36.3636363636364, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788044, Length=374, Percent_Identity=34.2245989304813, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1787560, Length=403, Percent_Identity=34.7394540942928, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789016, Length=338, Percent_Identity=34.0236686390533, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1786991, Length=394, Percent_Identity=30.9644670050761, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1786349, Length=334, Percent_Identity=29.0419161676647, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25144271, Length=369, Percent_Identity=33.3333333333333, Blast_Score=215, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI25144274, Length=260, Percent_Identity=38.0769230769231, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI32564660, Length=433, Percent_Identity=27.9445727482679, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71992977, Length=356, Percent_Identity=28.9325842696629, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17541228, Length=435, Percent_Identity=22.7586206896552, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324432, Length=389, Percent_Identity=35.73264781491, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323470, Length=390, Percent_Identity=30.2564102564103, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324386, Length=320, Percent_Identity=29.375, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321456, Length=440, Percent_Identity=23.1818181818182, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357415, Length=385, Percent_Identity=34.2857142857143, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI21356575, Length=413, Percent_Identity=31.4769975786925, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI28574759, Length=449, Percent_Identity=28.2850779510022, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI161085790, Length=456, Percent_Identity=28.2894736842105, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI281366494, Length=310, Percent_Identity=26.4516129032258, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24667139, Length=310, Percent_Identity=26.4516129032258, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24667143, Length=310, Percent_Identity=26.4516129032258, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR010164
- InterPro:   IPR017747
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.82 [H]

Molecular weight: Translated: 50777; Mature: 50777

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREPPEHILNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFL
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHH
EYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQL
HHHHHHCCCCCCCEEEEECCCCCHHHCCCCHHHHHHHCCCCEEECCCCCCEEHHHHHHHH
AKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKF
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCE
TFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDD
EEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCH
VAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENV
HHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEEHHHHHCCCCCCCEEEECCCCC
QPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLTCAAALATINVLL
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
EQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQ
HCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
RVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA
HHHEEEECCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIREPPEHILNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFL
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHH
EYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQL
HHHHHHCCCCCCCEEEEECCCCCHHHCCCCHHHHHHHCCCCEEECCCCCCEEHHHHHHHH
AKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKF
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCE
TFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDD
EEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCH
VAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENV
HHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCCEEEEHHHHHCCCCCCCEEEECCCCC
QPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLTCAAALATINVLL
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
EQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQ
HCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHH
RVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA
HHHEEEECCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA