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The map label for this gene is istB [H]

Identifier: 218701696

GI number: 218701696

Start: 3561115

End: 3561915

Strand: Direct

Name: istB [H]

Synonym: ECIAI39_3413

Alternate gene names: 218701696

Gene position: 3561115-3561915 (Clockwise)

Preceding gene: 218701692

Following gene: 218701700

Centisome position: 69.39

GC content: 52.68

Gene sequence:

>801_bases
GTGATGCATGAGCTGGAAGTCCTGCTGAGTCGCCTGAAAATGGAGCATCTGAGTTATCACGTTGAAAGCCTGCTGGAACA
GGCAGCTAAAAAAGAGCTGAACTACCGGGAGTTCCTGTGCATGGCGCTACAGCAGGAATGGAACGGCAGGCATCAGCGCG
GTATGGAGTCCAGGCTGAAGCAGGCTCGTCTGCCGTGGGTCAAAACGCTGGAGCAGTTCGACTTTACCTTCCAGCCGGGC
ATCGACCGTAAGGTTGTCCGGGAACTGGCTGGTCTGGCGTTCGTGGAGCGCAGCGAAAACGTGATCCTGCTGGGACCTCC
TGGTGTCGGAAAAACTCATCTGGCCATAGCTCTTGGCGTGAAAGCGGTGGATGCGGGACATCGGGTACTGTTTATGCCAC
TGGACAGACTGATCGCGACACTGATGAAAGCGAAACAGGAAAACCGGCTGGAGCGTCAGCTGCAGCAACTGAGTTATGCC
CGGGTGTTGATCCTGGATGAAATAGGCTATCTGCCGATGAACAGAGAGGAAGCCAGCCTGTTCTTCCGGCTACTGAACCG
TCGATATGAAAAAGCGAGCATCATACTGACGTCAAACAAAGGGTTCGCAGACTGGGGAGAAATGTTCGGAGATCACGTGC
TGGCAACAGCGATACTGGATCGGTTGCTACATCACTCAACCACGCTGAATATCAAAGGAGAGAGTTACCGGTTAAAAGAG
AAACGTAAAGCTGGAGTGCTGACCAAAAACACAACGCCAATCAGTGATGATGAAATGGTGAAAAGCGGACAGCATCAGTA
A

Upstream 100 bases:

>100_bases
CATCGTCTGGCTGGCAGACAGTGCCGGAGCATCACGCCCCGCTCTGGCAGCAGGTCAGTCAGGTGGAACATCGACCACTG
AGTGCCTATGAGGAGCTGTT

Downstream 100 bases:

>100_bases
CGAAAGTATCTTAGCGGGCATGAAAATGGCAAATAACGGTCAAACATCGTGGCGTTGACAACGGGCACTGCGTTTATCAG
GATGATGCCGCTGCTGCTGA

Product: transposase IstB, IS21

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MMHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPG
IDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYA
RVLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKE
KRKAGVLTKNTTPISDDEMVKSGQHQ

Sequences:

>Translated_266_residues
MMHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPG
IDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYA
RVLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKE
KRKAGVLTKNTTPISDDEMVKSGQHQ
>Mature_266_residues
MMHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPG
IDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYA
RVLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKE
KRKAGVLTKNTTPISDDEMVKSGQHQ

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 30660; Mature: 30660

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
QARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGV
HHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
KAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYARVLILDEIGYLPMNREEASL
HEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
FFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKE
HHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
KRKAGVLTKNTTPISDDEMVKSGQHQ
HHHCCCEECCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MMHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
QARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIALGV
HHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
KAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYARVLILDEIGYLPMNREEASL
HEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
FFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKE
HHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHH
KRKAGVLTKNTTPISDDEMVKSGQHQ
HHHCCCEECCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]