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The map label for this gene is cysA [C]

Identifier: 218701637

GI number: 218701637

Start: 3501657

End: 3502340

Strand: Direct

Name: cysA [C]

Synonym: ECIAI39_3345

Alternate gene names: 218701637

Gene position: 3501657-3502340 (Clockwise)

Preceding gene: 218701636

Following gene: 218701638

Centisome position: 68.23

GC content: 55.12

Gene sequence:

>684_bases
GTGGATATGGTTACGTTAGAACAGTTCCGCTATTGCCCGACACACTCAACGCATCCTCCGTTCTGTTATGACTTTCACTA
TGTCAAACCGGGGATGGTCGCCATCTTTGGTGACAACGGCAGTGGCAAAAGTACGCTGGCGCAGCTGATGGCGGGCTGGT
ATCCGGACTTTCTACCAGGAGAGATAACCGGCACGGGGACGCTTCTCGGTACGCCCATTGGGCGTCTCCCGCTTAATGAA
CAATCCGCCACCATCCAGCTCGTCCAGCAATCGCCCTATTTGCAGCTCTCCGGCTGTACCTTTAGCGTGGAAGAAGAAGT
TGCCTTTGGGCCAGAAAATCTGTGTCTTGCTGAAAAAGAGATTATGGCGCGGATTGACGCCGCCCTGGCCCTGACCGAGT
GCCAGCCGTTGCGCCATCGTCACCCGGCAACACTCTCCGGTGGAGAGACACAGCGCGTGGTCATCGCCTGCGCCATCGCG
ATGCAGCCAAAACTCTTAATTCTTGATGAAGCCTTTAGCCGCCTGACGCCGCAGGCCAGCGAAATGCTGTTGCAGCGCTT
ACAACACTGGGCGTCTGAACGTGGTTCGCTGATTATTCTTTTTGAACGTCATCGCACACCTTTCCTGAACTACTGCCAGC
AAGCGTGGCAATTGCAAAACGGAGCGCTCCAGCCACTATGTTAA

Upstream 100 bases:

>100_bases
TCAACAATAGGCGCACTACGTTATGGGCACCAGCCGATAGCACCCTACAACGCGTAGCACGTTACACCATGATCTTGCTG
ATGCTGGCAGAATTCGGAGC

Downstream 100 bases:

>100_bases
CGTTAAATCAGATAACTTATCGCTGGCCTGGCGCGGCAACGGATTGTCTTTGCGACATTTCTCTGCAACTCAAACAAGGT
GAATGGTTGGCGCTGACCGG

Product: putative ATP-binding protein of ABC transport system

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MDMVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTLAQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNE
QSATIQLVQQSPYLQLSGCTFSVEEEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIA
MQPKLLILDEAFSRLTPQASEMLLQRLQHWASERGSLIILFERHRTPFLNYCQQAWQLQNGALQPLC

Sequences:

>Translated_227_residues
MDMVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTLAQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNE
QSATIQLVQQSPYLQLSGCTFSVEEEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIA
MQPKLLILDEAFSRLTPQASEMLLQRLQHWASERGSLIILFERHRTPFLNYCQQAWQLQNGALQPLC
>Mature_227_residues
MDMVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTLAQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNE
QSATIQLVQQSPYLQLSGCTFSVEEEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIA
MQPKLLILDEAFSRLTPQASEMLLQRLQHWASERGSLIILFERHRTPFLNYCQQAWQLQNGALQPLC

Specific function: Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system [H]

COG id: COG1122

COG function: function code P; ABC-type cobalt transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transporter domains [H]

Homologues:

Organism=Homo sapiens, GI4505771, Length=205, Percent_Identity=29.2682926829268, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI9961252, Length=205, Percent_Identity=29.2682926829268, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI9961250, Length=208, Percent_Identity=29.8076923076923, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI42741659, Length=205, Percent_Identity=30.7317073170732, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI73747915, Length=209, Percent_Identity=35.4066985645933, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788761, Length=166, Percent_Identity=31.3253012048193, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1786653, Length=224, Percent_Identity=29.4642857142857, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787029, Length=191, Percent_Identity=29.8429319371728, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI87081791, Length=164, Percent_Identity=31.0975609756098, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787792, Length=186, Percent_Identity=32.7956989247312, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1789891, Length=197, Percent_Identity=29.4416243654822, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081709, Length=162, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787370, Length=212, Percent_Identity=26.4150943396226, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788225, Length=218, Percent_Identity=29.8165137614679, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI48994883, Length=196, Percent_Identity=28.5714285714286, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17543740, Length=206, Percent_Identity=31.0679611650485, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17541710, Length=172, Percent_Identity=31.9767441860465, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17565938, Length=182, Percent_Identity=32.967032967033, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI115534520, Length=224, Percent_Identity=28.125, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=202, Percent_Identity=30.1980198019802, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653245, Length=180, Percent_Identity=32.7777777777778, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281363262, Length=201, Percent_Identity=31.8407960199005, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136662, Length=222, Percent_Identity=30.6306306306306, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 25277; Mature: 25277

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTLAQLMAGWYPDFLPG
CCCEEHHHHHCCCCCCCCCCEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
EITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVEEEVAFGPENLCLAEKE
CCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCEEEECHHHCCCCCHHHHHHHH
IMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQPKLLILDEAFSRLTPQAS
HHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHCCCHHH
EMLLQRLQHWASERGSLIILFERHRTPFLNYCQQAWQLQNGALQPLC
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDMVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTLAQLMAGWYPDFLPG
CCCEEHHHHHCCCCCCCCCCEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
EITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVEEEVAFGPENLCLAEKE
CCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCEEEECHHHCCCCCHHHHHHHH
IMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQPKLLILDEAFSRLTPQAS
HHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHCCCHHH
EMLLQRLQHWASERGSLIILFERHRTPFLNYCQQAWQLQNGALQPLC
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]