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The map label for this gene is ygfA

Identifier: 218701619

GI number: 218701619

Start: 3482324

End: 3482872

Strand: Direct

Name: ygfA

Synonym: ECIAI39_3327

Alternate gene names: 218701619

Gene position: 3482324-3482872 (Clockwise)

Preceding gene: 218701618

Following gene: 218701622

Centisome position: 67.85

GC content: 51.91

Gene sequence:

>549_bases
ATGATTCGGCAACGCCGTCGTACGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGAC
TTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAG
AACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAAT
TACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAACTGGATGTGCGTGACGTGCTACCCCT
TTCCCGATTAGACGTGTTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTT
ATGATCGGACCTTACAAAACTGGCAGCGCTATCAAATGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAA
CTTCCCGTTGAAGAGTGGGATATCCCACTTCCTGCGGTGGTTACACCGTCGAAAGTCTGGGAGTGGTAA

Upstream 100 bases:

>100_bases
GCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTAT
CCCGACAAGAAATCCGCAAA

Downstream 100 bases:

>100_bases
GGGCGACACACCTGCATCGCCCTGATTGACATCGTTGATTCTTTAACCTAATTTAGTGAGTAAGGGTAAGGGTGGATGAC
TCCTCCCCTGAGACTGACTG

Product: putative ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MIRQRRRTLTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLN
YHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQRYQMQPVGYAHDCQLVEK
LPVEEWDIPLPAVVTPSKVWEW

Sequences:

>Translated_182_residues
MIRQRRRTLTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLN
YHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQRYQMQPVGYAHDCQLVEK
LPVEEWDIPLPAVVTPSKVWEW
>Mature_182_residues
MIRQRRRTLTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLN
YHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQRYQMQPVGYAHDCQLVEK
LPVEEWDIPLPAVVTPSKVWEW

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Homo sapiens, GI5453746, Length=181, Percent_Identity=24.3093922651934, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789278, Length=182, Percent_Identity=97.8021978021978, Blast_Score=368, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: NA

Molecular weight: Translated: 21185; Mature: 21185

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQRRRTLTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHCC
KRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAF
CEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHH
DEYGQRLGMGGGFYDRTLQNWQRYQMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVW
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCHHHC
EW
CC
>Mature Secondary Structure
MIRQRRRTLTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAG
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCHHHHHHHHHCC
KRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAF
CEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHH
DEYGQRLGMGGGFYDRTLQNWQRYQMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVW
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCHHHC
EW
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]