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The map label for this gene is yagI [H]

Identifier: 218701464

GI number: 218701464

Start: 3304163

End: 3305017

Strand: Direct

Name: yagI [H]

Synonym: ECIAI39_3164

Alternate gene names: 218701464

Gene position: 3304163-3305017 (Clockwise)

Preceding gene: 218701455

Following gene: 218701465

Centisome position: 64.38

GC content: 52.28

Gene sequence:

>855_bases
ATGAGATGGAATTTATATTGTGGGATATATCACCTTTTCCCCACGTCATTATGTGGAACACTTCAGCAATATCATCAGGA
GAAACGTATGGCAGAAACCTCTTCAACCACCGCAGGCGCACAAACGTTATTACGTGGACTGGCGGTTCTTAATGCTGTTT
ATAACGGCTGCCATGACCTAAAAAGTATCGGCGAGTTCACCGGCACGACCCGGAGCACAACCCATCGTTTAGTCACCGTT
CTGGTGGAACAACGCTACCTGCGCCATGTCCCAACGCAGGGCTACCAACTGGGTGCCAAACTGATCGAATTCGGTGCCAG
AGCACTGGAAAGCACCTCGCTGTACGAAATTGCCCAGCCCGTTTTGCAACGCCTTGCCCGTTACACCCTGGACACCGTGC
ATCTGGGGATCGTTGAAGGCGACGAAGTGCTGTATCTGGAGAAGATCAACAGTCAGCGCGGGCTGGAAATGCGCTCACGC
CCAGGCCATCGCATGCCACTGGCAATCACCGGCATCGGCAAAGCATTGATCCTCAACCGAACAGAAGAAGAGTGGCGCAC
GCTGTTTAAGACCTGTGGTGATGAAACAAAACTGGGCGCATTTATTCAGAACATGCGTCGTTATGCCGCCAGTGGTTTTG
CTTTTGATCTGGAAGAGAACGAGCCGACTATCCGTTGCGTTGCAGCGCCGGTTTACAACGCCCGGGATGAAATTGTGGCG
GCTATTTCTGTCGCCAGTACCACCACGTATATGTCGCTGGCGCGTCTGGAAGAACTGGCACCTTACGTCAAATCCTGTGC
AGAAGAGATATCCGCTGAACTGGGCTGGGGTAAGCACGTCCGCAAAGATAAATAA

Upstream 100 bases:

>100_bases
ACAAAATAGAATTGTGCTGTACAAACGTCCAGTTTGAAAGAAGTTTCCTGCGAGGCGCTACGCAAAACGATCGTTAATTT
TCATTATTTTATCCCACAAT

Downstream 100 bases:

>100_bases
ACCATGATTAAAGGAAACGACTAATGCCGGGAGCACCCTCATTTATTGCCCTGGACTGGGGAACGTCATCGCTTCGGGCA
TGGCGTTTTGGCGAATCACC

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRWNLYCGIYHLFPTSLCGTLQQYHQEKRMAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRSTTHRLVTV
LVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLDTVHLGIVEGDEVLYLEKINSQRGLEMRSR
PGHRMPLAITGIGKALILNRTEEEWRTLFKTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVA
AISVASTTTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK

Sequences:

>Translated_284_residues
MRWNLYCGIYHLFPTSLCGTLQQYHQEKRMAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRSTTHRLVTV
LVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLDTVHLGIVEGDEVLYLEKINSQRGLEMRSR
PGHRMPLAITGIGKALILNRTEEEWRTLFKTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVA
AISVASTTTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK
>Mature_284_residues
MRWNLYCGIYHLFPTSLCGTLQQYHQEKRMAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRSTTHRLVTV
LVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLDTVHLGIVEGDEVLYLEKINSQRGLEMRSR
PGHRMPLAITGIGKALILNRTEEEWRTLFKTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVA
AISVASTTTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=246, Percent_Identity=32.1138211382114, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788131, Length=218, Percent_Identity=32.1100917431193, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1789999, Length=272, Percent_Identity=30.1470588235294, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788581, Length=255, Percent_Identity=30.1960784313725, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87082359, Length=260, Percent_Identity=26.9230769230769, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790752, Length=223, Percent_Identity=28.2511210762332, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=25.4980079681275, Blast_Score=91, Evalue=8e-20,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31866; Mature: 31866

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWNLYCGIYHLFPTSLCGTLQQYHQEKRMAETSSTTAGAQTLLRGLAVLNAVYNGCHDL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KSIGEFTGTTRSTTHRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQRLARYTLDTVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNR
HHHHHHHHHHHHHEEEEECCCCEEEEEHHCCCCCCCCCCCCCCCCCEEEECCCHHEEECC
TEEEWRTLFKTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHH
AISVASTTTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRWNLYCGIYHLFPTSLCGTLQQYHQEKRMAETSSTTAGAQTLLRGLAVLNAVYNGCHDL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KSIGEFTGTTRSTTHRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLQRLARYTLDTVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNR
HHHHHHHHHHHHHEEEEECCCCEEEEEHHCCCCCCCCCCCCCCCCCEEEECCCHHEEECC
TEEEWRTLFKTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVA
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHH
AISVASTTTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]