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The map label for this gene is metL

Identifier: 218701355

GI number: 218701355

Start: 3180772

End: 3183204

Strand: Reverse

Name: metL

Synonym: ECIAI39_3054

Alternate gene names: 218701355

Gene position: 3183204-3180772 (Counterclockwise)

Preceding gene: 218701356

Following gene: 218701351

Centisome position: 62.03

GC content: 57.75

Gene sequence:

>2433_bases
ATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAACTGCATAAATTTGGTGGCAGTAGTCTGGCTGATGTGAAGTG
TTATTTGCGTGTCGCGGGCATTATGGCGGAGTACTCTCAGCCTGACGATATGATGGTGGTTTCCGCCGCCGGTAGCACCA
CTAACCAGTTGATTAACTGGTTGAAACTAAGCCAGACCGATCGTCTCTCTGCGCATCAGGTTCAACAAACGCTACGTCGT
TATCAGTGCGATCTGATTAGCGGCCTGCTACCCGCTGAAGAAGCCGATAGCCTCATTAGCGCTTTTGTCAGCGATCTGGA
ACGCCTGGCGGCGCTGCTCGACAGCGGTATTAACGATGCGGTGTATGCGGAAGTGGTGGGCCACGGTGAAGTGTGGTCGG
CGCGCCTGATGTCTGCGGTACTTAATCAACAAGGGCTGCCAGCGGCCTGGCTTGATGCCCGCGAGTTTTTACGCGCTGAA
CGCGCCGCGCAGCCGCAGGTTGATGAAGGGCTTTCTTACCCGTTGCTGCAACAGCTACTGGTACAACATCCGGGCAAACG
TCTGGTGGTGACCGGGTTTATCAGCCGTAACAACGCCGGTGAAACGGTGCTGCTGGGGCGTAACGGTTCCGACTATTCCG
CGACACAAATCGGTGCGCTGGCGGGGGTTTCCCGCGTAACCATTTGGAGCGATGTCGCCGGGGTATACAGTGCCGACCCG
CGTAAAGTGAAAGATGCCTGTCTGCTGCCATTGCTGCGTCTTGATGAAGCCAGTGAACTGGCGCGTCTGGCGGCCCCCGT
TCTTCACGCCCGTACTTTACAGCCAGTATCCGGTAGTGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAG
GTTCCACGCGCATTGAACGCGTGCTGGCTTCCGGTACTGGCGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATT
GAGTTTCAGGTGCCTGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGTGCTCAGGTGCG
CCCACTGGCGGTTGGCGTACATAATGATCGCCAGTTGCTGCAATTTTGCTACACCTCGGAAGTGGCCGATAGTGCGCTGA
AAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGTCTGCGCCAGGGGCTGGCACTGGTGGCGATGGTCGGGGCGGGC
GTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAGTTTACCTGGCAGTCCGA
TGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGACCTACCGAAAGCCTGATTCAGGGCCTGCATCAGTCCGTCTTCC
GCGCAGAAAAACGCATTGGCCTGGTGCTGTTCGGCAAGGGCAATATCGGTTCCCGCTGGCTGGAACTGTTCGCCCGTGAG
CAGAGTACGCTTTCGGCGCGTACCGGCTTTGAATTTGTGCTGGCAGGCGTGGTGGACAGCCGCCGCAGCCTGTTGAGCTA
TGACGGGCTGGACGCCAGCCGCGCGTTAGCCTTCTTCAACGATGAAGCCGTCGAGCAGGATGAAGAGTCGCTGTTCCTGT
GGATGCGCGCCCATCCGTATGATGATTTAGTGGTGCTGGACGTTACCGCCAGCCAGCAGCTTGCTGATCAGTATCTTGAT
TTCGCCAGCCACGGTTTCCACGTCATCAGTGCCAACAAACTGGCAGGAGCGAGCGACAGCAATAAATATCGCCAGATCCA
CGACGCCTTCGAAAAAACCGGGCGTCACTGGCTATACAACGCCACCGTCGGCGCAGGTTTGCCGATCAACCACACCGTGC
GCGATCTGATCGACAGCGGCGACACTATTTTGTCGATCAGCGGGATCTTCTCCGGCACACTCTCCTGGCTGTTCCTGCAA
TTCGACGGTAGCGTGCCGTTTACCGAGCTGGTGGATCAGGCGTGGCAGCAGGGCTTAACTGAACCTGACCCGCGTGATGA
CCTCTCCGGCAAAGACGTGATGCGCAAGCTGGTGATTCTGGCGCGTGAAGCGGGTTACAACATTGAACCGGACCAGGTGC
GTGTTGAATCACTGGTGCCAACCCATTGCGAAGGTGGAAGCATCGACCATTTCTTTGAAAATGGCGATGAACTTAATGAG
CAGATGGTACAACGGTTGGAAGCGGCCCGTGAAATGGGGCTGGTGTTGCGCTACGTGGCGCGTTTCGATGCCAACGGCAA
AGCGCGAGTAGGCGTGGAAGCGGTGCGAGAAGACCATCCGCTGGCATCACTACTACCGTGCGATAACGTCTTTGCCATCG
AAAGCCGCTGGTATCGCGACAACCCGCTGGTGATCCGCGGGCCAGGTGCAGGGCGCGACGTCACCGCCGGGGCGATTCAG
TCGGATATTAACCGTCTGGCGCAGTTGTTGTAG

Upstream 100 bases:

>100_bases
TCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGG
GCTGCAAACAAGGGGTAAAA

Downstream 100 bases:

>100_bases
TGATTATTGAACAAAAGCTGGCAGTTGCCAGCTTTTGTTGTCACCTCTAAACCACCTCCTGCGGAGGTGGTGATTGATTG
TCATTTTGCCTTTGGTCAGT

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRR
YQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLI
EFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAG
VTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLD
FASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQ
FDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNE
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQ
SDINRLAQLL

Sequences:

>Translated_810_residues
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRR
YQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLI
EFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAG
VTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLD
FASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQ
FDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNE
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQ
SDINRLAQLL
>Mature_809_residues
SVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRY
QCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAER
AAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPR
KVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIE
FQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGV
TRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFAREQ
STLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDF
ASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQF
DGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQ
MVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQS
DINRLAQLL

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=810, Percent_Identity=99.8765432098765, Blast_Score=1647, Evalue=0.0,
Organism=Escherichia coli, GI1786183, Length=821, Percent_Identity=30.5724725943971, Blast_Score=333, Evalue=4e-92,
Organism=Escherichia coli, GI1790455, Length=288, Percent_Identity=31.25, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322599, Length=370, Percent_Identity=35.1351351351351, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320893, Length=491, Percent_Identity=23.2179226069246, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88919; Mature: 88788

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINW
CCCCHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDA
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAERAAQPQVDEGLSYPLLQQLL
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH
VQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
HHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCHHEEHHHHHHHHCCCCC
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIER
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHH
VLASGTGARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLL
HHHCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
QFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE
EFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPY
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCEEEEEECCCC
DDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYN
CCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLT
ECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCC
EPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEHHHCCCCCCCCCCCHHHHCCCHHHHH
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHCCCCCHHHCCCCCEEEECCCEECC
NPLVIRGPGAGRDVTAGAIQSDINRLAQLL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINW
CCCHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHH
LKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAFVSDLERLAALLDSGINDA
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAERAAQPQVDEGLSYPLLQQLL
HHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHH
VQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADP
HHCCCCEEEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCHHEEHHHHHHHHCCCCC
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIER
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCHHHHHH
VLASGTGARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLL
HHHCCCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
QFCYTSEVADSALKILDEAGLPGELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCE
EFTWQSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFARE
EEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
QSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPY
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCEEEEEECCCC
DDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYN
CCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEE
ATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLT
ECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHCEEEEEEEECCCCCHHHHHHHHHHCCCC
EPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNE
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEHHHCCCCCCCCCCCHHHHCCCHHHHH
QMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHCCCCCHHHCCCCCEEEECCCEECC
NPLVIRGPGAGRDVTAGAIQSDINRLAQLL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]