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The map label for this gene is yfkN [H]

Identifier: 218701349

GI number: 218701349

Start: 3173360

End: 3174919

Strand: Reverse

Name: yfkN [H]

Synonym: ECIAI39_3048

Alternate gene names: 218701349

Gene position: 3174919-3173360 (Counterclockwise)

Preceding gene: 218701351

Following gene: 218701348

Centisome position: 61.86

GC content: 47.69

Gene sequence:

>1560_bases
TTGTTTATGAAAATTAAAATGCTTGCTGCGGGTATCGCATTAACACTGCCGTTTTGGGCTTGCGCCAAAGATGTCACCAT
CATTTATACCAACGACCTCCATGCACATGTAGAGCCTTATAAAGTGCCGTGGATTGCTGACGGTAAACGAGATATTGGCG
GCTGGGCAAATATCACTACGCTTGTTAAACAAGAAAAAGCTAAAAACAAAGCGACCTGGTTTTTTGATGCGGGTGACTAT
TTTACCGGGCCGTATATCAGCAGCCTGACTAAAGGCAAGGCGATTATCGATATTATGAATACCATGCCATTCGATGCGGT
CACTATAGGTAATCATGAATTTGATCACGGCTGGGACAATACGTTATTACAGTTGAGTCAGGCAAAATTCCCGATTGTGC
AGGGTAATGTTTTTTATCAGAACAGCAGTAAATCATTCTGGGATAAGCCCTATACCATCATTGAAAAAGACGGCGTGAAA
ATTGGCGTGATTGGTTTGCACGGTGTATTTGCCTTTAATGATACGGTATCTGCGGCAACGCGAGTGGGTATTGAGGCGCG
TGATGAAATTAAATGGCTACAACGTTATATCGATGAACTCAAAGGCAAGGTTGATTTGACCGTTGCGCTGATTCACGAAG
GTGTTCCGGCCCGCCAGTCCAGTATGGGGGGCACAGATGTGCGCCGCGCACTGGATAAAGATATTCAGACGGCAAGTCAG
GTGAAAGGGCTGGATATTTTGATCACCGGGCATGCACATGTGGGTACGCCGGAACCGATTAAAGTCGGCAATACGCTCAT
CCTCTCCACTGACAGCGGTGGGATTGATGTCGGTAAACTGGTACTGGACTACAAAGAGAAGCCGCATGATTTTACGGTGA
AAAACTTCGAGCTTAAAACCATTTACGCCGACGAGTGGAAGCCCGATCCGCAAACAAAACAGGTGATCGACGGCTGGAAT
AAAAAGCTTGATGAAGTGGTGCAACAAACGGTCGCGCAATCGCCGGTTGAATTAAAACGTGCCTATGGTGAATCGGCTTC
CCTCGGGAACCTGGCGGCTGATGCTTTACTGGTAGCGGCGGGTAAAAACACCCAATTGGCGTTAACCAACTCTGGCGGCA
TTCGCAATGAGATCCCCGCAGGGGCGATTACGATGGGCGGCGTCATCAGTACCTTCCCGTTCCCCAACGAACTGGTGACG
ATGGAACTCACGGGTAAACAATTACGCAGTTTGATGGAACACGGCGCAAGTTTAAGTAATGGCGTTTTACAGGTATCGAA
AGGCCTGGAAATGAAGTACGACAGCAGCAAACCGGTTGGTCAGCGGGTAATCACACTGACTCTGAATGGCAAACCCATTG
AAGATGCGACAGTTTATTCTATTGCCACCCAGAGTTTCCTTGCTGATGGTGGAGATGGTTTTACCGCCTTTACCGAAGGG
AAAGCGCGTAACACAACGGGCGGTTATTACGTTTATCACGCCGTGGTTGATTACTTCAAAGCGGGTAACACCATCACGGA
TGAACAGATCAACGGTATGCGCGTGAAAGATATCAAGTAA

Upstream 100 bases:

>100_bases
TTGATTTTGTCCTACAGTTATCGCCTCTGGATGTGAGTATTCTCACATAAAAAAGCCTTTCAGAACGTAATGTAGCAATC
CTTTATCTCACTGGAAGGGA

Downstream 100 bases:

>100_bases
AAGTACGTATTGAGTTGATTCATTTGTTTGTTACCCGTTGATTTTGCAGATCCAGTATTCGGCCTACGCGAGGGTGCAGG
CCGAATCAGATGCCTTAGCA

Product: putative phosphoesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MFMKIKMLAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDY
FTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVK
IGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHDFTVKNFELKTIYADEWKPDPQTKQVIDGWN
KKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVT
MELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYSIATQSFLADGGDGFTAFTEG
KARNTTGGYYVYHAVVDYFKAGNTITDEQINGMRVKDIK

Sequences:

>Translated_519_residues
MFMKIKMLAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDY
FTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVK
IGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHDFTVKNFELKTIYADEWKPDPQTKQVIDGWN
KKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVT
MELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYSIATQSFLADGGDGFTAFTEG
KARNTTGGYYVYHAVVDYFKAGNTITDEQINGMRVKDIK
>Mature_519_residues
MFMKIKMLAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITTLVKQEKAKNKATWFFDAGDY
FTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVK
IGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHDFTVKNFELKTIYADEWKPDPQTKQVIDGWN
KKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLVAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVT
MELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYSIATQSFLADGGDGFTAFTEG
KARNTTGGYYVYHAVVDYFKAGNTITDEQINGMRVKDIK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=529, Percent_Identity=26.0869565217391, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1786687, Length=538, Percent_Identity=24.1635687732342, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1790658, Length=560, Percent_Identity=22.6785714285714, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI221329836, Length=540, Percent_Identity=26.6666666666667, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24641187, Length=514, Percent_Identity=27.2373540856031, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19921980, Length=488, Percent_Identity=25.8196721311475, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652512, Length=488, Percent_Identity=25.8196721311475, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161076508, Length=487, Percent_Identity=26.0780287474333, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922446, Length=543, Percent_Identity=23.7569060773481, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24654424, Length=543, Percent_Identity=23.7569060773481, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922444, Length=558, Percent_Identity=24.5519713261649, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28573524, Length=552, Percent_Identity=22.8260869565217, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 56728; Mature: 56728

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00786 5_NUCLEOTIDASE_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMKIKMLAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITT
CEEEEEEHHCCHHHHCCHHHHCCCEEEEEECCCEECCCCEECCEEECCCCCCCCHHHHHH
LVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDN
HHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEHHHCCCCCEEEECCCCCCCCCCH
TLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSAAT
HHHHHHCCCCCEEECEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCHHHHHH
RVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
HCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHDFTVKNFELKT
CCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHHHEEEECCCCCCCEEEEEEEEEE
IYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLVAA
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHCEEEEEE
GKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASLSN
CCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCC
GVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYSIATQSFLADGGDGFTAFTEG
CEEEECCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHCCCCCCCEEEECC
KARNTTGGYYVYHAVVDYFKAGNTITDEQINGMRVKDIK
CCCCCCCCEEHHHHHHHHHHCCCEECCCCCCCEEEECCC
>Mature Secondary Structure
MFMKIKMLAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIADGKRDIGGWANITT
CEEEEEEHHCCHHHHCCHHHHCCCEEEEEECCCEECCCCEECCEEECCCCCCCCHHHHHH
LVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPFDAVTIGNHEFDHGWDN
HHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEHHHCCCCCEEEECCCCCCCCCCH
TLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSAAT
HHHHHHCCCCCEEECEEEEECCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCHHHHHH
RVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQ
HCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
VKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHDFTVKNFELKT
CCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHHHEEEECCCCCCCEEEEEEEEEE
IYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLVAA
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHCEEEEEE
GKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASLSN
CCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCC
GVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYSIATQSFLADGGDGFTAFTEG
CEEEECCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHCCCCCCCEEEECC
KARNTTGGYYVYHAVVDYFKAGNTITDEQINGMRVKDIK
CCCCCCCCEEHHHHHHHHHHCCCEECCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]