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The map label for this gene is rffC

Identifier: 218701296

GI number: 218701296

Start: 3106066

End: 3106740

Strand: Reverse

Name: rffC

Synonym: ECIAI39_2997

Alternate gene names: 218701296

Gene position: 3106740-3106066 (Counterclockwise)

Preceding gene: 218701297

Following gene: 218701295

Centisome position: 60.54

GC content: 55.11

Gene sequence:

>675_bases
GTGCCCGTCCGCGCCAGTATTGAGCCACTAACCTGGGAAAACGCCTTCTTTGGTGTTAACAGCGCCATTGTGCGCATTAC
GTCTGAAGCACCGCTCCTGACGCCAGACGCCTTAGCGCCGTGGTCACGGGTGCAGGCCAAAATTGCGGCATCAAATACGG
GTGAACTGGATGCCCTGCAACAGCTGGGATTCTCCCTGGTAGAAGGTGAAGTTGATTTGGCGCTACCCGTGAACAATGCC
AGTGATAGCGGTGCTGTAGTGGCACAAGAGACCGATATTCCCGCATTACGTCAGTTAGCCAGCGCCGCATTTGCGCAAAG
CCGTTTTCGTGCGCCGTGGTATGCGCCTGACGCCAGCAGTCGCTTTTATGCACAGTGGATTGAAAATGCCGTGCGCGGCA
CCTTTGATCATCAATGTCTGATTTTACGTGCGGCGTCCGGCGATATTCGCGGTTATGTCTCTTTACGGGAACTCAATGCG
ACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGC
TCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACTGCCGCGCTTAAACGATACATACAAAGTGGTGCGA
ATGTAGAAAGCACCGCGTACTGGTTATACAGGTGA

Upstream 100 bases:

>100_bases
ATCGCCTGGCGTAACGGCTGGCTCGATGACGAGGGTGTGAAGCGTGCTGCCAGTTCATTAGCGAAAACTGGCTACGGCCA
ATATCTGCTGGAGTTACTTC

Downstream 100 bases:

>100_bases
TCACATGATTCCATTTAACGCACCGCCGGTGGTGGGAACCGAACTCGACTATATGCAGTCGGCAATGGGTAGCGGCAAAC
TGTGCGGCGATGGTGGTTTT

Product: TDP-fucosamine acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRVQAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNA
SDSGAVVAQETDIPALRQLASAAFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVESTAYWLYR

Sequences:

>Translated_224_residues
MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRVQAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNA
SDSGAVVAQETDIPALRQLASAAFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVESTAYWLYR
>Mature_223_residues
PVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRVQAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNAS
DSGAVVAQETDIPALRQLASAAFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNAT
DARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVESTAYWLYR

Specific function: Transaminase converting thymidine diphosphate(TDP)-4- keto-6-deoxy-D-glucose to TDP-4-amino-4,6-dideoxy-D-galactose, the immediate precursor of the ECA sugar TDP-Fuc4NAc

COG id: COG0454

COG function: function code KR; Histone acetyltransferase HPA2 and related acetyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Escherichia coli, GI145693203, Length=224, Percent_Identity=100, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFFC_ECOLI (P27832)

Other databases:

- EMBL:   M87049
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A65183
- RefSeq:   AP_004007.1
- RefSeq:   YP_026256.2
- ProteinModelPortal:   P27832
- SMR:   P27832
- STRING:   P27832
- EnsemblBacteria:   EBESCT00000004461
- EnsemblBacteria:   EBESCT00000018308
- GeneID:   948298
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5597
- KEGG:   eco:b3790
- EchoBASE:   EB1424
- EcoGene:   EG11455
- eggNOG:   COG0454
- GeneTree:   EBGT00050000010056
- HOGENOM:   HBG416100
- OMA:   FQLVEGE
- ProtClustDB:   PRK10975
- BioCyc:   EcoCyc:TDPFUCACTRANS-MONOMER
- BioCyc:   MetaCyc:TDPFUCACTRANS-MONOMER
- Genevestigator:   P27832
- InterPro:   IPR000182
- InterPro:   IPR012752
- InterPro:   IPR016181
- InterPro:   IPR002110
- InterPro:   IPR020683
- Gene3D:   G3DSA:3.40.630.30
- TIGRFAMs:   TIGR02382

Pfam domain/function: PF00583 Acetyltransf_1; SSF55729 Acyl_CoA_acyltransferase

EC number: NA

Molecular weight: Translated: 24221; Mature: 24089

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS51186 GNAT; PS50088 ANK_REPEAT ; PS50297 ANK_REP_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRVQAKIAASNTGELDALQ
CCCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHEEEECCCCCCHHHHH
QLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASAAFAQSRFRAPWYAPDASS
HCCCEEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
RFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNATDARIGLLAGRGAGAELMQT
HHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHH
ALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVESTAYWLYR
HHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure 
PVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRVQAKIAASNTGELDALQ
CCCCCCCCCEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHEEEECCCCCCHHHHH
QLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASAAFAQSRFRAPWYAPDASS
HCCCEEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
RFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNATDARIGLLAGRGAGAELMQT
HHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHH
ALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVESTAYWLYR
HHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065