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The map label for this gene is rffT

Identifier: 218701293

GI number: 218701293

Start: 3102603

End: 3103682

Strand: Reverse

Name: rffT

Synonym: ECIAI39_2994

Alternate gene names: 218701293

Gene position: 3103682-3102603 (Counterclockwise)

Preceding gene: 218701294

Following gene: 218701292

Centisome position: 60.48

GC content: 53.24

Gene sequence:

>1080_bases
ATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTCTGCGGTTTTTCAACCACGCGCT
GGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTTGGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTT
CTGTGCAATTTTTCCCTGGGAAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTAACCGTCAGCAGCGATTT
TTTTTCCACGGTCAGTTCAATCCCACACTGTGGCTGGCTCTGCTGAGTGGTGGCATTAAGCCCAGCCAGTTTTTCTGGCA
TATCTGGGGAGCAGACCTGTATGAGCTTTCCAGTGGCTTGAGATATAAGCTTTTTTACCCGTTGCGTCGCCTGGCGCAAA
AGCGAGTCGGCTGTGTATTTGCCACCCGCGGCGATTTGAGCTTTTTTGCCAAAACGCACCCAAAGGTGCGGGGCGAACTG
CTGTACTTCCCGACGCGGATGGACCCTTCTCTCAATACGATGGCGAACGATCGGCAACGTGAAGGGAAAATGACCATTCT
GGTGGGCAACTCCGGCGACCGCAGCAATGAGCATATTGCTGCCTTGCGCGCCGTTCATCAGCAATTTGGCGATACGGTAA
AAGTGGTGGTGCCGATGGGATATCCGCCTAATAACGAAGCGTACATCGAGGAAGTTCGTCAGGCGGGGCTGGAGTTATTC
AGCGAAGAAAATCTGCAAGTTCTGAGCGAAAAACTGGAATTTGACGCCTATCTGACGCTACTTCGTCAGTGCGATCTTGG
TTACTTTATTTTTGCCCGCCAGCAGGGCATTGGTACGCTGTGCTTACTGATTCAGGCGGGCATTCCTTGTGTGCTTAACC
GGGAAAATCCGTTCTGGCAGGATATGACGGAACAACATTTGCCGGTGCTGTTTACTACCGACGATCTCAACGAGGATATT
GTGCGTGAAGCGCAGCGCCAGTTGGCGTCGGTGGATAAAAACACCATTGCCTTCTTTAGCCCGAACTATCTGCAAGGCTG
GCAGCGGGCGTTGGCGATTGCCGCCGGGGAGGTTGCATGA

Upstream 100 bases:

>100_bases
CTGCGCATGGTGCACTGGGCGCGGCGCAGGCCTATATGGCAACCTATATCGTCTATTTTTCTCTTTGTTGTGGCGTGTTT
TTACTCTGGCGTAGGCGGGC

Downstream 100 bases:

>100_bases
GTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATTGCCACGCTGACCTGGTTTGAGTTT
CGCCGTATGCGCTTTAACTT

Product: 4-alpha-L-fucosyltransferase

Products: NA

Alternate protein names: TDP-Fuc4NAc:lipid II Fuc4NAc transferase; Fuc4NAc transferase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MTVLIHVLGSDIPHHNRTVLRFFNHALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRF
FFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
SEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDI
VREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA

Sequences:

>Translated_359_residues
MTVLIHVLGSDIPHHNRTVLRFFNHALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRF
FFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
SEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDI
VREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA
>Mature_358_residues
TVLIHVLGSDIPHHNRTVLRFFNHALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFF
FHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELL
YFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELFS
EENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIV
REAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc (Lipid III), the third lipid-linked intermediate involved in ECA synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 56 family [H]

Homologues:

Organism=Escherichia coli, GI48994971, Length=359, Percent_Identity=98.6072423398329, Blast_Score=737, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009993 [H]

Pfam domain/function: PF07429 Fuc4NAc_transf [H]

EC number: NA

Molecular weight: Translated: 40595; Mature: 40464

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLIHVLGSDIPHHNRTVLRFFNHALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPG
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCC
KKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGL
HHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCHHHEEEEECCCHHHHHCCCC
RYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELLYFPTRMDPSLNTMANDRQR
EEEEHHHHHHHHHHHHCEEEEECCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCHHC
EGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
SEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQ
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEEECCCCCCHH
DMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TVLIHVLGSDIPHHNRTVLRFFNHALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPG
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCC
KKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGL
HHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCHHHEEEEECCCHHHHHCCCC
RYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELLYFPTRMDPSLNTMANDRQR
EEEEHHHHHHHHHHHHCEEEEECCCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHCCHHC
EGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
SEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQ
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCEEECCCCCCHH
DMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAGEVA
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA