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The map label for this gene is yifK

Identifier: 218701290

GI number: 218701290

Start: 3098935

End: 3100320

Strand: Reverse

Name: yifK

Synonym: ECIAI39_2991

Alternate gene names: 218701290

Gene position: 3100320-3098935 (Counterclockwise)

Preceding gene: 218701291

Following gene: 218701289

Centisome position: 60.41

GC content: 53.17

Gene sequence:

>1386_bases
ATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCACCATTGG
CGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGC
TATTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCTGTTACCGGTTCGTTCGCCGTTTATGCG
CATCGTTATATGAGCCCGTTTTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCAGA
AATCACCGCCATTGGCGTTTATGTCCAGTTCTGGTTCCCGGAGATGGCGCAATGGATACCGGCATTGATCGCGGTGGCGC
TGGTAGCATTGGCGAATCTGGCGGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTTGCGATGATCAAAGTCACCACG
ATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAA
TCTCACAGAGCATGGCGGTTTCTTTGCGGGGGGCTGGAAAGGGTTCCTGACCGCGCTGTGTATTGTGGTGGCGTCCTACC
AGGGCGTGGAGCTGATTGGCATTACTGCCGGAGAAGCGAAGAATCCGCAGGTGACGCTGCGCAGTGCCGTAGGCAAGGTG
CTGTGGCGAATCCTGATTTTCTACGTAGGCGCGATTTTCGTTATCGTCACCATCTTCCCGTGGAATGAAATAGGCAGCAA
CGGCAGCCCGTTCGTACTGACTTTCGCCAAAATCGGCATTACCGCAGCGGCGGGTATTATCAACTTTGTGGTGCTGACGG
CTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCG
GCGGCAATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTC
ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGC
CATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGTCGTGCGCATAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCA
ATCCTGTTCCCGTGGGCCAACTACGTAACAATGGCATTCCTGATTTGCGTTTTGATCGGCATGTACTTTAATGAAGATAC
GCGTATGTCGCTGTTTGTCGGCATCATCTTTATGCTGGCGGTGACGGCGATTTATAAAGTTTTTGGGCTTAATTGCCACG
GTAAAGCGCATAAACTGGAGGAATAA

Upstream 100 bases:

>100_bases
TTGCCATTTCTCCTGAATTACGAAAACATTCGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATG
CAAACACAACACGAGGATTT

Downstream 100 bases:

>100_bases
GCAGCAAAACGCACAAACCGTAACCAAACGCGCAATTTATTTAAAAAGGGACTAGACAGAGGAGTGGGAAGTCCGTATTA
TCCACCCCCGCAACGGCGCT

Product: putative transport protein YifK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYA
HRYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKV
LWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLP
AAMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRS
ILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNCHGKAHKLEE

Sequences:

>Translated_461_residues
MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYA
HRYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKV
LWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLP
AAMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRS
ILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNCHGKAHKLEE
>Mature_460_residues
ADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAH
RYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTTI
IVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKVL
WRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPA
AMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRSI
LFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNCHGKAHKLEE

Specific function: Probable amino-acid or metabolite transport protein [H]

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI48994972, Length=461, Percent_Identity=99.7830802603037, Blast_Score=915, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=429, Percent_Identity=44.0559440559441, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1786302, Length=431, Percent_Identity=40.1392111368909, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1786602, Length=437, Percent_Identity=45.9954233409611, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1790653, Length=447, Percent_Identity=39.3736017897092, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI87081915, Length=392, Percent_Identity=39.2857142857143, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1788480, Length=435, Percent_Identity=39.0804597701149, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI87081708, Length=448, Percent_Identity=37.5, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1789017, Length=464, Percent_Identity=32.7586206896552, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI2367353, Length=460, Percent_Identity=25.2173913043478, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324061, Length=438, Percent_Identity=35.1598173515982, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6320772, Length=439, Percent_Identity=34.3963553530752, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324924, Length=414, Percent_Identity=32.3671497584541, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324990, Length=447, Percent_Identity=30.4250559284116, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322892, Length=437, Percent_Identity=30.8924485125858, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6324059, Length=439, Percent_Identity=33.0296127562642, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322967, Length=415, Percent_Identity=30.3614457831325, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321629, Length=404, Percent_Identity=27.970297029703, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319824, Length=436, Percent_Identity=28.4403669724771, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6321053, Length=421, Percent_Identity=29.2161520190024, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6319608, Length=422, Percent_Identity=28.9099526066351, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324981, Length=410, Percent_Identity=30.4878048780488, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6319543, Length=400, Percent_Identity=29.25, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324553, Length=397, Percent_Identity=30.9823677581864, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319542, Length=403, Percent_Identity=27.2952853598015, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320717, Length=439, Percent_Identity=24.6013667425968, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6320251, Length=400, Percent_Identity=26.5, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320364, Length=555, Percent_Identity=23.7837837837838, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI221512776, Length=360, Percent_Identity=21.9444444444444, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24666159, Length=360, Percent_Identity=21.9444444444444, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50347; Mature: 50215

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIM
CCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFP
HHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTTIIVMIVIGLGVIFFGFGNGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
QSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKV
CCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
LWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCN
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
SGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVF
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRSILFPWANYVTMAFLICVLIG
EEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNCHGKAHKLEE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIM
CCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFP
HHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTTIIVMIVIGLGVIFFGFGNGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
QSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKV
CCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
LWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCN
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
SGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVF
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRSILFPWANYVTMAFLICVLIG
EEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNCHGKAHKLEE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503 [H]