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The map label for this gene is hemX

Identifier: 218701286

GI number: 218701286

Start: 3092135

End: 3093316

Strand: Direct

Name: hemX

Synonym: ECIAI39_2987

Alternate gene names: 218701286

Gene position: 3092135-3093316 (Clockwise)

Preceding gene: 218701285

Following gene: 218701287

Centisome position: 60.25

GC content: 53.64

Gene sequence:

>1182_bases
ATGACGGAACAAGAAAAAACCTCCGCCGTGGTTGAAGAGACCAGGGAGGCCGTGGACACCACGTCACAACCTGTCGCAAC
AGAAAAAAAGAGTAAGAACAATACCGCATTGATTCTCAGCGCGGTGGCTATCGCTATTGCTCTGGCGGCGGGCGTCGGTT
TGTATGGCTGGGGTAAACAACAGGCCGTCAATCAGACTGCCACCAGCGATGCCCTGGCTAATCAACTGACGGCACTGCAA
AAAGCCCAGGAGAGCCAAAAAGCTGAGCTGGAAGGCATTATCAAGCAACAAGCTGTACAGCTTGAGCAGGCGAATCGTCA
GCAAGAAACGCTGGCAAAACAGCTGGATGAAGTCCAACAAAAGGTCGCGACTATTTCCGGCAGCGATGCTAAAACCTGGC
TGCTGGCTCAGGCTGATTTCCTGGTGAAACTCGCCGGACGGAAGCTGTGGAGCGATCAGGACGTCACGACCGCTGCAGCG
TTGCTGAAAAGTGCAGACGCCAGCCTGGCGGATATGAATGACCCGAGTCTGATTACCGTTCGCCGGGCAATTACCGATGA
TATCGCCAGCCTTTCTGCGGTATCGCAGGTGGATTATGACGGCATTATCCTTAAGCTTAATCAGCTTTCAAATCAGGTAG
ATAACCTGCGTCTGGCTGATAATGACAGCGATGGTTCGCCGATGGATTCCGACGGTGAAGAGCTTTCCAGTTCCATCAGC
GAATGGCGTATCAATCTGCAAAAAAGCTGGCAGAACTTTATGGACAACTTCATTACGATTCGCCGTCGTGATGACACCGC
CGTACCGCTGTTAGCGCCAAATCAGGATATCTATCTGCGCGAAAATATTCGCTCTCGCCTGCTGGTCGCTGCACAAGCTG
TACCGCGTCATCAGGAAGAGACTTATCGCCAGGCGCTGGAGAACGTCTCCACCTGGGTACGTGCTTACTACGATACTGAT
GATGCCACCACCAAAGCGTTCCTCGACGAGGTGGACCAGTTAAGCCAGCAAAATATCTCGATGGATCTCCCGGAAACCCT
GCAAAGCCAGGCGATGCTGGAAAAACTGATGCAGACTCGCGTGCGTAACCTGCTGGCACAACCGGCAGCGGGGGCAACGG
AAGCTAAACCTGCACCTGCACCGCAAGCTGATACTCCGGCAGCCGCGCCGCAAGGAGAATAA

Upstream 100 bases:

>100_bases
CGCCCGGGAACTGGGCTGGCAAGACATTAAGGTCGCCGATAACGCTGACAACGATGCGCTTTTACGGGCATTACAATAAC
TCTCATAACAGGAAGCCATA

Downstream 100 bases:

>100_bases
CCATGCTAAAAGTGTTATTGCTCTTTGTTTTGCTGATTGCGGGGATCGTGGTTGGCCCGATGATTGCCGGTCATCAGGGG
TACGTATTGATTCAGACCGA

Product: putative uroporphyrinogen III C-methyltransferase

Products: NA

Alternate protein names: Urogen-III methylase; ORF X [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAIAIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQ
KAQESQKAELEGIIKQQAVQLEQANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRLADNDSDGSPMDSDGEELSSSIS
EWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQDIYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTD
DATTKAFLDEVDQLSQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGATEAKPAPAPQADTPAAAPQGE

Sequences:

>Translated_393_residues
MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAIAIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQ
KAQESQKAELEGIIKQQAVQLEQANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRLADNDSDGSPMDSDGEELSSSIS
EWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQDIYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTD
DATTKAFLDEVDQLSQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGATEAKPAPAPQADTPAAAPQGE
>Mature_392_residues
TEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAIAIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQK
AQESQKAELEGIIKQQAVQLEQANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAAL
LKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRLADNDSDGSPMDSDGEELSSSISE
WRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQDIYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDD
ATTKAFLDEVDQLSQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGATEAKPAPAPQADTPAAAPQGE

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790235, Length=393, Percent_Identity=98.9821882951654, Blast_Score=778, Evalue=0.0,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007470 [H]

Pfam domain/function: PF04375 HemX [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 42948; Mature: 42817

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAIAIALAAGVGLYGWGKQ
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHH
QAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLEQANRQQETLAKQLDEVQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAALLKSADASLADMNDPSLITV
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCCEEHH
RRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRLADNDSDGSPMDSDGEELSSSIS
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHH
EWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQDIYLRENIRSRLLVAAQAVPRHQEE
HHHHHHHHHHHHHHHHHEEEEECCCCCCCEECCCCCEEEHHHHHHHHHHHHHHCCCHHHH
TYRQALENVSTWVRAYYDTDDATTKAFLDEVDQLSQQNISMDLPETLQSQAMLEKLMQTR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VRNLLAQPAAGATEAKPAPAPQADTPAAAPQGE
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAIAIALAAGVGLYGWGKQ
CCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHH
QAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLEQANRQQETLAKQLDEVQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAALLKSADASLADMNDPSLITV
HHHHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCCEEHH
RRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRLADNDSDGSPMDSDGEELSSSIS
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCEECCCCCCCCCCCCCHHHHHHHHH
EWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQDIYLRENIRSRLLVAAQAVPRHQEE
HHHHHHHHHHHHHHHHHEEEEECCCCCCCEECCCCCEEEHHHHHHHHHHHHHHCCCHHHH
TYRQALENVSTWVRAYYDTDDATTKAFLDEVDQLSQQNISMDLPETLQSQAMLEKLMQTR
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VRNLLAQPAAGATEAKPAPAPQADTPAAAPQGE
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3062586; 3054815; 1379743; 9278503; 9298646 [H]