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The map label for this gene is fhlA

Identifier: 218701223

GI number: 218701223

Start: 3026163

End: 3028241

Strand: Direct

Name: fhlA

Synonym: ECIAI39_2920

Alternate gene names: 218701223

Gene position: 3026163-3028241 (Clockwise)

Preceding gene: 218701222

Following gene: 218701224

Centisome position: 58.97

GC content: 52.09

Gene sequence:

>2079_bases
ATGTCATATACACCGATGAGTGATCTCGGACAACAAGGGTTGTTCGACATCACTCGGACACTATTGCAGCAGCCCGATCT
GGCCTCGCTGTGTGAGGCTCTTTCGCAACTGGTAAAGCGTTCTGCGCTCGCCGACAACGCGGCTATTGTGTTGTGGCAAG
CGCAGACTCAACGTGCGTCTTATTACGCGTCGCGTGAAAAAGACACCCCCATTAAATATGAAGACGAAACTGTTCTGGCA
CACGGTCCGGTACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCA
GCTGGCCGCAGGTGGGCTATACCCCAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCATATTTTTGGTG
GCTGTGAATTTATTCGTTATGACGATCGCCCGTGGAGTGAAAAAGAGTTCAATCGCCTGCAAACTTTTACGCAGATCGTT
TCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCGACTATGAGTTGTTATGCCGGGAACGCGATAACTT
CCGCATCCTGGTCGCCATCACCAACGCGGTGCTTTCCCGCCTGGATATGGACGAACTGGTCAGCGAAGTCGCCAAAGAAA
TCCATTACTATTTCGACATTGACGATATCAGCATTGTCTTACGCAGCCACCGTAAAAACAAACTCAACATCTACTCCACT
CACTATCTTGATAAACAGCATCCCGCCCACGAACAGAGCGAAGTCGATGAAGCCGGAACCCTCACCGAACGCGTCTTCAA
AAGTAAAGAGATGCTGTTGATTAATCTCCACGAGCGGGACGATTTAGCCCCCTATGAACGCATGTTGTTCGACACCTGGG
GCAACCAGATTCAAACCTTGTGCCTGTTACCGCTGATGTCTGGCGACACCATGCTGGGCGTGCTGAAACTGGCGCAATGC
GAAGAGAAAGTATTTACCACTACCAATCTGAGCTTACTGCGCCAGATTGCCGAACGTGTGGCAATCGCTGTCGATAACGC
CCTCGCCTATCAGGAAATTCATCGTCTGAAAGAACGGCTGGTTGATGAAAACCTCGCCCTGACCGAGCAGCTCAACAATG
TTGATAGTGAATTTGGCGAGATTATTGGCCGCAGCGAAGCCATGTACAGCGTACTTAAACAAGTTGAAATGGTGGCGCAA
AGTGACAGTACCGTTCTGATCCTCGGTGAAACTGGTACAGGTAAAGAGCTGATTGCCCGCGCGATCCATAATCTCAGTGG
GCGTAATAATCGCCGCATGGTCAAAATGAACTGCGCGGCGATGCCTGCCGGATTGCTGGAAAGCGATCTATTTGGTCATG
AGCGTGGGGCTTTTACCGGTGCCAGCGCTCAGCGCATTGGTCGTTTTGAACTGGCGGATAAAAGCTCACTATTCCTCGAT
GAAGTGGGCGATATGCCGCTGGAGTTACAGCCGAAGTTGCTGCGTGTATTACAGGAACAGGAGTTTGAACGTCTCGGCAG
CAACAAAATCATTCAGACGGACGTGCGTCTAATCGCCGCAACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGT
TCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCG
CTGCTGGCGAAAGCCTTTACCTTCAAAATTGCCCGTCGTCTGGGGCGCAATATCGACAGCATTCCTGCCGAGACGCTGCG
CATCCTGAGCAACATGGAATGGCCGGGCAACGTGCGTGAGCTGGAAAACGTCATCGAGCGCGCGGTATTGCTAACACGCG
GCAACGTGCTGCAGCTGTCATTGCCAGATATTGCTTTACCAGAACCTGAAACGCCGCCTGCCGCAACGGTTGTCGCCCAG
GAGGGCGAAGATGAATATCAGTTGATTGTTCGCGTGCTGAAAGAAACAAACGGCGTGGTTGCCGGGCCTAAAGGCGCTGC
GCAACGTCTGGGGCTGAAACGCACGACTCTGCTGTCACGGATGAAGCGACTGGGAATTGATAAATCGGCATTGATTTAA

Upstream 100 bases:

>100_bases
GCCGAACCGCTTCCGCGTATATGCTAATAAAATTCTAAATCTCCTATAGTTAGTCAATGACCTTTTGCACCGCTTTGCGG
TGCTTTCCTGGAAGAACAAA

Downstream 100 bases:

>100_bases
CAGATGTGGTTATACTCGATCAACATTCAATGAAAGGAGGCAGTATGGCAGTTTCAGCAAGAAATCAATTAACCGGAACA
GTTAGCGCAGTAGCAATGGG

Product: DNA-binding transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLA
HGPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQC
EEKVFTTTNLSLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIP
LLAKAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQ
EGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI

Sequences:

>Translated_692_residues
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLA
HGPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQC
EEKVFTTTNLSLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQ
SDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIP
LLAKAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQ
EGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
>Mature_691_residues
SYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAH
GPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVS
VVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYSTH
YLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCE
EKVFTTTNLSLLRQIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQS
DSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDE
VGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPL
LAKAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQE
GEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=692, Percent_Identity=99.2774566473989, Blast_Score=1415, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=566, Percent_Identity=51.0600706713781, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=46.9648562300319, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1788550, Length=322, Percent_Identity=46.2732919254658, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1788905, Length=307, Percent_Identity=46.9055374592834, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI87082152, Length=366, Percent_Identity=39.344262295082, Blast_Score=244, Evalue=9e-66,
Organism=Escherichia coli, GI1790299, Length=365, Percent_Identity=41.0958904109589, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=41.875, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87081872, Length=265, Percent_Identity=44.9056603773585, Blast_Score=210, Evalue=3e-55,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=38.855421686747, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=333, Percent_Identity=36.6366366366366, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=35.8267716535433, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87081858, Length=326, Percent_Identity=31.9018404907975, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78397; Mature: 78266

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRAS
CCCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
YYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHY
HHHCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
CLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQSRVVNNVDYELL
EEEEECCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHEEECCHHHHHHHHHCCCCCEEEEEE
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRE
EEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
SYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALADNAAIVLWQAQTQRAS
CCCCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHH
YYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFCETWPQLAAGGLYPKFGHY
HHHCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
CLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIVSVVTEQIQSRVVNNVDYELL
EEEEECCCCCEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
CRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYFDIDDISIVLRSHRKNKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHEEECCHHHHHHHHHCCCCCEEEEEE
HYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINLHERDDLAPYERMLFDTWGNQIQTL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
CLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLRQIAERVAIAVDNALAYQEIHRLKERL
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVDSEFGEIIGRSEAMYSVLKQVEMVAQSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNNRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEECHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKIIQTDVRLIAATNRDLKKMVADREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHEEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPEDIPLLAKAFTFKIARRLGRNIDSIPAETLRILSNMEWPGNVRE
EEEEEEEECCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPDIALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVV
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEE
AGPKGAAQRLGLKRTTLLSRMKRLGIDKSALI
ECCCHHHHHCCCHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]