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The map label for this gene is hycH

Identifier: 218701210

GI number: 218701210

Start: 3014861

End: 3015271

Strand: Reverse

Name: hycH

Synonym: ECIAI39_2906

Alternate gene names: 218701210

Gene position: 3015271-3014861 (Counterclockwise)

Preceding gene: 218701211

Following gene: 218701209

Centisome position: 58.75

GC content: 52.07

Gene sequence:

>411_bases
ATGAGTGAAAAGGTGGTGTTCAGTCAACTGAGCCGTAAATTTATTGATGAGAACGATGCCACGCCCGCCGAGGCGCAGCA
GGTGGTCTATTACAGCCTGGCGATTGGTCACCACCTTGGGGTTATCGATTGCCTGGAAGCCGCGCTCACCTGCCCGTGGG
ATGAATATCTGGCATGGATTGCCACTCTGGAGGCGGGCAGTGAAGCCCGCCGTAAAATGGAAGGCGTGCCGAAGTACGGT
GAGATCGTCATCGACATTAACCATGTGCCGATGCTGGCTAACGCATTCGATAAAGCCCGGGCAGCGCAAACTTCGCAGCA
GAAAGAATGGAGTACCATGCTGTTAAGTATGCTGCATGATATTTATCAGGAAAATGCCATCTATTTGATGGTGAGGAGAT
TGCGTGACTGA

Upstream 100 bases:

>100_bases
GTGAAGAACAGGTTGCACGCTGGCTGGAAGCGGAAAACGATCCGCGTCTGAACGAGATTGTCAGCCATCTGAATCATGTT
GTTGAAGAGGCGCGTATCCG

Downstream 100 bases:

>100_bases
CGTTTTACTCTGTGTTGGCAATAGCATGATGGGCGATGATGGCGCAGGCCCGCTGCTGGCGGAAAAGTGCGCCGCCGCGC
CGAAAGGTAACTGGGTGGTG

Product: hydrogenase 3 maturation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYG
EIVIDINHVPMLANAFDKARAAQTSQQKEWSTMLLSMLHDIYQENAIYLMVRRLRD

Sequences:

>Translated_136_residues
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYG
EIVIDINHVPMLANAFDKARAAQTSQQKEWSTMLLSMLHDIYQENAIYLMVRRLRD
>Mature_135_residues
SEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGE
IVIDINHVPMLANAFDKARAAQTSQQKEWSTMLLSMLHDIYQENAIYLMVRRLRD

Specific function: Seems to be required for the conversion of a precursor form of the large subunit of hydrogenlyase (hycE) into a mature form [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli hyfJ [H]

Homologues:

Organism=Escherichia coli, GI1789073, Length=136, Percent_Identity=98.5294117647059, Blast_Score=282, Evalue=7e-78,
Organism=Escherichia coli, GI87082116, Length=131, Percent_Identity=47.3282442748092, Blast_Score=122, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010005
- ProDom:   PD021295 [H]

Pfam domain/function: PF07450 HycH [H]

EC number: NA

Molecular weight: Translated: 15491; Mature: 15360

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWI
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
ATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAAQTSQQKEWSTMLLSMLHD
HHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYQENAIYLMVRRLRD
HHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWI
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHH
ATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAAQTSQQKEWSTMLLSMLHD
HHHHCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYQENAIYLMVRRLRD
HHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]