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The map label for this gene is emrB

Identifier: 218701181

GI number: 218701181

Start: 2983467

End: 2985005

Strand: Direct

Name: emrB

Synonym: ECIAI39_2876

Alternate gene names: 218701181

Gene position: 2983467-2985005 (Clockwise)

Preceding gene: 218701180

Following gene: 218701192

Centisome position: 58.13

GC content: 56.27

Gene sequence:

>1539_bases
ATGCAACAGCAAAAACCGCTGGAAGGCGCACAACTGGTCATTATGACGATTGCGCTGTCACTGGCGACATTCATGCAGGT
GCTGGACTCCACCATTGCTAACGTGGCGATCCCCACTATCGCCGGGAATCTGGGCTCATCACTCAGTCAGGGAACGTGGG
TGATCACTTCTTTCGGGGTGGCGAATGCCATCTCGATCCCGCTTACCGGCTGGCTGGCAAAGCGCGTCGGGGAAGTGAAA
TTGTTCCTCTGGTCAACCATCGCCTTTGCTATTGCGTCGTGGGCGTGTGGTGTCTCCAGCAGCCTGAATATGCTGATCTT
CTTCCGCGTGATTCAGGGGATTGTCGCCGGGCCGCTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAG
CCAAACGCTCGATCGCGCTGGCATTGTGGTCGATGACGGTGATTGTTGCGCCAATTTGCGGCCCGATCCTCGGCGGTTAT
ATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTACCAATTGGCGTGGCGGTGGTGTTGATGACCCTGCA
AACCCTGCGCGGACGTGAAACCCGCACCGAACGGCGGCGGATTGATGCCGTGGGGCTGGCACTGCTGGTTATTGGTATCG
GCAGCCTGCAGATTATGCTCGACCGCGGTAAAGAGCTGGACTGGTTCTCATCACAGGAAATTATCATCCTCACCGTGGTG
GCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACTGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTC
GCGCAACTTCACCATTGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGT
TGTTGCAGGAGGTCTACGGTTACACGGCAACGTGGGCAGGTCTGGCCTCTGCGCCGGTAGGAATTATTCCGGTGATCCTG
TCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTG
CTTCTACTGGCGTGCCTATACCTTTGAACCGGGTATGGATTTTGGCGCGTCGGCCTGGCCGCAGTTTATCCAGGGTTTTG
CGGTGGCCTGCTTCTTTATGCCGCTAACCACCATTACGCTGTCCGGTTTGCCACCGGAACGACTGGCGGCAGCATCGAGC
CTCTCCAACTTTACGCGAACGCTGGCGGGGTCTATCGGCACGTCGATAACCACGACCATGTGGACCAACCGCGAGTCGCT
GCACCATGCGCAGTTGACTGAGTCGGTAAACCCGTTCAACCCGAATGCCCAGGCGATGTATAGTCAACTGGAAGGGCTTG
GGATGACGCAACAGCAGGCATCAGGCTGGATTGCCCAGCAGATCACCAATCAGGGGCTGATTATTTCTGCCAATGAGATC
TTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCCTTTGGCGCAGGTGGCGG
CGGAGGCGGTGCGCACTAA

Upstream 100 bases:

>100_bases
GTAGCGGTAAGCACCGCGCGTGAAATCAGCCTGGCACCTGTCAATAAACTGATCGACGATATCGTAAAAGCTAACGCTGG
CTAATCCAGAGGTGCGTGTG

Downstream 100 bases:

>100_bases
GTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCT
GCAACTTCTCTTTCGGCAGC

Product: multidrug efflux system protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Sequences:

>Translated_512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
>Mature_512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVK
LFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=512, Percent_Identity=99.8046875, Blast_Score=1028, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=503, Percent_Identity=62.4254473161034, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=426, Percent_Identity=31.924882629108, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=29.2079207920792, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=27.2941176470588, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=532, Percent_Identity=22.5563909774436, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319770, Length=313, Percent_Identity=26.1980830670927, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=30, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=29.1970802919708, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55607; Mature: 55607

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLI
HHEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCHHHHHHH
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
FGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTM
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
ECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCCH
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSLNMLIFFRVIQGIVAGPLI
HHEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
PLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQIMLDRGKELDWFSSQEIIILTVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCHHHHHHH
AVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGIIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAYTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
FGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTM
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTNRESLHHAQLTESVNPFNPNAQAMYSQLEGLGMTQQQASGWIAQQITNQGLIISANEI
ECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCCH
FWMSAGIFLVLLGLVWFAKPPFGAGGGGGGAH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]