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The map label for this gene is ecoRIIM [H]

Identifier: 218701133

GI number: 218701133

Start: 2934628

End: 2936103

Strand: Reverse

Name: ecoRIIM [H]

Synonym: ECIAI39_2825

Alternate gene names: 218701133

Gene position: 2936103-2934628 (Counterclockwise)

Preceding gene: 218701135

Following gene: 218701130

Centisome position: 57.21

GC content: 44.44

Gene sequence:

>1476_bases
ATGAGTGATAGAGTTTCACCCATTAGACCCCTTGGAGGAATTATGTCTGAATTTGAAGTGTTGGCACAGCATCTGCTGAA
GGAAGCCGAGGCGGAAGAAAAGCTGCGACAGGAAAATGATAAAAAGCTTATCGAGAAAGTGCTGGAAATCTATGATCAGA
AGTATGTGGCTGAATTACTGAGAAAAGTCGGAAAAAATGAGTGGAGCCGTGAAACCATTAACCGCTGGATTAATGGCAAG
TGTTTACCAAAATCGTTGACGTCAGTAGAAGAGTCTCTTCTGCGTAAGATGCTACCAGAACCACCTGCAAACCATCCGGA
ATATGCTTTCCGCTTCATTGACTTATTTGCTGGAATTGGAGGGATACGAAAAGGTTTTGAGGCTATTGGAGGCCAGTGTG
TTTTTACCAGTGAATGGAATAAAGATGCTGTGCGTACATACAAGGCCAACTGGTTTAACGATGAACAGGTGCATAAATTC
AATCTCGATATTCGGGAAGTCACGTTGAGTGATAAAACCGATGTATTGGAAACGGATGCTTATGCATATATTGATGAGCA
TGTGCCGGATCATGATGTGCTTCTTGCGGGGTTCCCGTGCCAGCCATTTAGTCTTGCTGGTGTTAGTAAGAAAAACTCAC
TTGGCCGTGCGCATGGTTTTGAATGTGAAGCACAAGGAACACTTTTTTTTGACGTGGCACGTATTATACGAGCAAAAAAA
CCGGCAATTTTTGTGCTGGAAAATGTCAAAAATCTGAAGAGCCATGATAAGGGTAAAACTTTCAAAGTCATTATGGAGAC
CCTTGACGAGCTGGGCTACGAAGTTGCCGATGCTGCAGAAATGGGAAAAAACGATCCAAAGATTATCGATGGGAAGCATT
TTTTGCCTCAGCATCGTGAACGCATTGTTCTGGTCGGATTTAGACGAGATCTGAATATTCACAAGGGGTTTACCTTGCGT
GATATCAGTCGTTTTTATCCGGAACACCGCCCATCATTTGGTGAATTGCTGGAGCCTGTAGTCGACAGTAAATATATACT
TACGCCAAAACTATGGGAGTACCTTTATAACTACGCCAAAAAGCATGCAGCCAAGGGGAATGGTTTTGGCTTTGGGTTGG
TCAATCCTGAGAATAAGGAAAGCATTGCACGCACACTTTCTGCCAGATATCACAAAGACGGATCAGAAATTCTTATTGAT
CGTGGTTGGGATATGGCAACGGGAGAGGCTGACTTTATGAATGAGAGTAATCAGGCTCGTCGACCACGCAGGCTGACTCC
CCGAGAGTGCGCACGCCTTATGGGATTTGAAAAACCTGGAGGGAAACCATTCCGTATTCCGGTTTCAGATACTCAGTCAT
ATCGGCAGTTCGGTAACTCAGTTGTGGTGCCAGTATTTGAAGCTGTTGCCAGACTTCTGGAACCCTATATCCTCAAAGCT
GTTTCTGCTGATGCTGGTAAGACTGGGCAGCCTTGA

Upstream 100 bases:

>100_bases
ATATAGAATGATTCTCTATGCCTAACAAAGCATCACTAGTGCTAGATTACGTTGTAGTCACTGTTTAAATATCCAGACAA
AACATTTCCTTCTGGGTTGA

Downstream 100 bases:

>100_bases
GAACACCTCCCGGCCTTTTAAGCCGGGAGTCAGCCTATGATAGTTCAGCGTAAAGCTCTATCAGCTCAGTAATGAATGCT
CCGAGCGTTATTAATTCTTC

Product: DNA cytosine methylase

Products: NA

Alternate protein names: M.EcoRII; Cytosine-specific methyltransferase EcoRII [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSDRVSPIRPLGGIMSEFEVLAQHLLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAELLRKVGKNEWSRETINRWINGK
CLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLFAGIGGIRKGFEAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKF
NLDIREVTLSDKTDVLETDAYAYIDEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKK
PAIFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIIDGKHFLPQHRERIVLVGFRRDLNIHKGFTLR
DISRFYPEHRPSFGELLEPVVDSKYILTPKLWEYLYNYAKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILID
RGWDMATGEADFMNESNQARRPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVPVFEAVARLLEPYILKA
VSADAGKTGQP

Sequences:

>Translated_491_residues
MSDRVSPIRPLGGIMSEFEVLAQHLLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAELLRKVGKNEWSRETINRWINGK
CLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLFAGIGGIRKGFEAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKF
NLDIREVTLSDKTDVLETDAYAYIDEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKK
PAIFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIIDGKHFLPQHRERIVLVGFRRDLNIHKGFTLR
DISRFYPEHRPSFGELLEPVVDSKYILTPKLWEYLYNYAKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILID
RGWDMATGEADFMNESNQARRPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVPVFEAVARLLEPYILKA
VSADAGKTGQP
>Mature_490_residues
SDRVSPIRPLGGIMSEFEVLAQHLLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAELLRKVGKNEWSRETINRWINGKC
LPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLFAGIGGIRKGFEAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKFN
LDIREVTLSDKTDVLETDAYAYIDEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKP
AIFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIIDGKHFLPQHRERIVLVGFRRDLNIHKGFTLRD
ISRFYPEHRPSFGELLEPVVDSKYILTPKLWEYLYNYAKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDR
GWDMATGEADFMNESNQARRPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVPVFEAVARLLEPYILKAV
SADAGKTGQP

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=442, Percent_Identity=68.552036199095, Blast_Score=624, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 55891; Mature: 55760

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRVSPIRPLGGIMSEFEVLAQHLLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAELL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKVGKNEWSRETINRWINGKCLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLFAGIG
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GIRKGFEAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKFNLDIREVTLSDKTDVLETDA
HHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCHHHCCH
YAYIDEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKK
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PAIFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIIDGKHFLPQHRE
CEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCEECCCCCCCCCCC
RIVLVGFRRDLNIHKGFTLRDISRFYPEHRPSFGELLEPVVDSKYILTPKLWEYLYNYAK
EEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHHH
KHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGEADFMNESNQAR
HHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCCCCC
RPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVPVFEAVARLLEPYILKA
CCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHH
VSADAGKTGQP
HCCCCCCCCCC
>Mature Secondary Structure 
SDRVSPIRPLGGIMSEFEVLAQHLLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAELL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKVGKNEWSRETINRWINGKCLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLFAGIG
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GIRKGFEAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKFNLDIREVTLSDKTDVLETDA
HHHHHHHHHCCEEEEECCCCCHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCHHHCCH
YAYIDEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKK
HHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
PAIFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIIDGKHFLPQHRE
CEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCCCEECCCCCCCCCCC
RIVLVGFRRDLNIHKGFTLRDISRFYPEHRPSFGELLEPVVDSKYILTPKLWEYLYNYAK
EEEEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECHHHHHHHHHHHH
KHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGEADFMNESNQAR
HHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCCCCC
RPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVPVFEAVARLLEPYILKA
CCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHH
VSADAGKTGQP
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029675 [H]