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The map label for this gene is yfiN

Identifier: 218701116

GI number: 218701116

Start: 2919688

End: 2920914

Strand: Direct

Name: yfiN

Synonym: ECIAI39_2808

Alternate gene names: 218701116

Gene position: 2919688-2920914 (Clockwise)

Preceding gene: 218701115

Following gene: 218701117

Centisome position: 56.89

GC content: 48.41

Gene sequence:

>1227_bases
ATGATGGATAACGATAATTCTCTTAATAAGCGCCCCACGTTTAAAAGAGCATTACGCAACATCAGTATGACCAGCATATT
TATCACTATGATGCTGATCTGGTTGCTGCTTTCCGTGACCTCGGTGCTGACCCTGAAACAGTACGCGCAAAAAAACCTGG
CACTGACAGCAGCAACAATGACTTACAGTCTGGAAGCAGCGGTCGTTTTTGCCGATGGCCCTGCAGCAACTCAAACACTG
GCAGCGCTGGGGAAGCAAGGGCAATTTTCAACTGCAGAAGTGCGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTA
CACCCGTAAGGATCCAGGCGATACTTTCAGCAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCACTCAGCCGATTC
GTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGCTCGATCAGCCATTTTATCTGGTTTTCGCTC
GCTGTCCTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTGGA
AGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCTAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTA
TCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTACAGGCC
AAAAATGCGCAGCTTCTACGTACCGCGCTACATGACCCATTAACCGGGCTGGCTAACCGCGCAGCGTTTCGTAGCGGCAT
CAACACGTTGATGAACAATTCCGATGCCCGAAAAACGTCGGCGTTACTATTTCTTGATGGCGATAATTTCAAATATATCA
ATGATACCTGGGGTCATGCGACGGGCGATAGAGTCTTGATTGAAATAGCAAAACGGTTAACTGAATTTGGCGGGCTGCGA
CATAAAGCATACCGCCTGGGCGGCGATGAATTCGCTATGGTGCTCTATGATGTACAATCGGAATCTGAAGTGCAGCAGAT
ATGCTCAGCACTGACACAAATTTTTAATCTCCCGTTTGATCTTCATAATGGCCATCAGACTACCATGACATTAAGCATTG
GTTACGCGATGACCATTGAGCACGCCTCTGCGGAAAATTTACAAGAGCTTGCCGATCACAATATGTATCAGGCCAAACAC
CAGCGTGCCGAAAAGCTGGTGAGATAA

Upstream 100 bases:

>100_bases
ATCCACAATAATGACGTCAGATTTGCCGTAAACCTGGATGCCTTATCGCGTAGCGGTGTAAAGGTCAACCCGGATGTCTT
AATGCTCGCACGGAAGAAAA

Downstream 100 bases:

>100_bases
CAAGGATATATCGATATGATAAAGCACCTGGTAGCACCCCTGATTTTCACCTCGCTAATACTTACTGGCTGCCAGTCCCC
TCAGGGAAAGTTTACTCCTG

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATQTL
AALGKQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPITQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLTEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKH
QRAEKLVR

Sequences:

>Translated_408_residues
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATQTL
AALGKQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPITQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLTEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKH
QRAEKLVR
>Mature_408_residues
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATMTYSLEAAVVFADGPAATQTL
AALGKQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPAPITQPIRHNGETIGEVRLTARDSSISHFIWFSL
AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLTEFGGLR
HKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKH
QRAEKLVR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=408, Percent_Identity=98.7745098039216, Blast_Score=835, Evalue=0.0,
Organism=Escherichia coli, GI145693134, Length=151, Percent_Identity=31.1258278145695, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=152, Percent_Identity=34.8684210526316, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=32.0512820512821, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788381, Length=100, Percent_Identity=42, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=30.0613496932515, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=32.7272727272727, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081977, Length=101, Percent_Identity=41.5841584158416, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786584, Length=150, Percent_Identity=30, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45993; Mature: 45993

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATM
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHH
TYSLEAAVVFADGPAATQTLAALGKQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFI
EEEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SHWLFPAPITQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRH
HHCCCCCCCCHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCC
TGDRVLIEIAKRLTEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFD
CCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
LHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKHQRAEKLVR
CCCCCCEEEEEEECEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLTLKQYAQKNLALTAATM
CCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHH
TYSLEAAVVFADGPAATQTLAALGKQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFI
EEEEEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
SHWLFPAPITQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRH
HHCCCCCCCCHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCC
TGDRVLIEIAKRLTEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFD
CCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
LHNGHQTTMTLSIGYAMTIEHASAENLQELADHNMYQAKHQRAEKLVR
CCCCCCEEEEEEECEEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]