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The map label for this gene is yfhK

Identifier: 218701069

GI number: 218701069

Start: 2864170

End: 2865597

Strand: Reverse

Name: yfhK

Synonym: ECIAI39_2760

Alternate gene names: 218701069

Gene position: 2865597-2864170 (Counterclockwise)

Preceding gene: 218701070

Following gene: 218701068

Centisome position: 55.84

GC content: 52.66

Gene sequence:

>1428_bases
TTGAAACGCTGGCCCGTTTTTCCCCGCTCATTACGACAACTGGTAATGCTGGCATTTTTGCTGATTCTGCTACCCCTGTT
GGTGCTGGCATGGCAAGCCTGGCAAAGCCTGAATGCGCTTAGCGATCAGGCGGCGCTGGTTAACCGCACTACGCTTATTG
ATGCCCGGCGCAGTGAAGCGATGACCAACGCGGCGCTGGAGATGGAGCGTAGCTACCGTCAGTATTGCGTGCTGGACGAC
CCTACGCTTGCGAAGGTTTATCAAAGCCAGCGCAAGCGTTACAGCGAAATGCTCGATGCCCACGCAGGCGTGCTGCCGGA
CGATAAACTCTACCAGGCATTACGTCATGATTTGAATAATCTGGCTCAACTTCAGTGTAACAACAGCGGTCCCGATGCTG
CTGCCGCCGCGCGTCTGGAAGCCTTTGCCAGTGCCAATACCGAAATGGTGCAGGCCACGCGCACAGTGGTGTTCTCTCGT
GGGCAGCAACTTCAGCGTGAAATCGCCGAACGTGGGCAATATTTTGGTTGGCAATCGCTGGTGCTATTTCTGGTGAGTCT
GGTGATGGTACTGCTTTTCACGATGATGATTATCGGTCCGGTGAAAAATATCGAGCGCATGATCAACCGGCTGGGGGAAG
GGCGTTCTCTGGGCAATAGCGTCTCGTTCAGTGGACCGAGCGAGTTACGCTCGGTTGGGCAACGTATTCTTTGGTTAAGT
GAGCGCCTGTCATGGCTGGAATCCCAACGCCATCAATTTTTAAGACATTTATCTCATGAATTAAAAACCCCGCTGGCGAG
TATGCGCGAGGGCACTGAATTACTGGCTGACCAGGTTGTCGGGCCGCTTACGCCCGAGCAAAAAGAGGTGGTGAGCATCC
TTGATAGCAGCAGCCGCAATTTGCAAAAACTGATCGAACAACTGCTTGATTACAACCGTAAACAAGCGGACAGTGCGGTG
GAACTGGAGAATGTTGAGTTAGCACCGCTGGTGGAGACAGTAGTTTCTGCTCATAGCCTGCCCGCACGGGCTAAAATGAT
GCATACCGACGTTGATCTCAAAGCAACAGCTTGCCTGGCGGAGCCAATGCTGCTGATGAGCGTACTGGATAATCTTTACT
CCAATGCGGTGCACTACGGGGCTGAATCCGGTAACATTTGCCTTCGCAGCAGTTTACATGGTGCGCGGGTTTATATTGAT
GTCATCAATACAGGCATGCCCATTCCGCAAGAGGAACGCGCCATGATCTTCGAACCCTTTTTTCAAGGAAGCCACCAGCG
AAAAGGGGCGGTGAAGGGCAGCGGTCTGGGATTAAGCATTGCCAGGGATTGTATTCGCCGTATGCAAGGGGAACTGTATC
TGGTCGACGAGAGCGGGCAAGATGTTTGTTTCCGCATTGAATTACCGTCGTCGAAAAACACGAAATAA

Upstream 100 bases:

>100_bases
CGCCTTATTCCATAACAAAGCCGGGTAATTCCCGGCTTTGTTGTATCTGAACTTCCCCTCGGTTAGCATCAGGCTATTCG
CGTCTGACGAGAGTAACACC

Downstream 100 bases:

>100_bases
TGCTAACAGCCCATCCCATCAGGCTATTTTATTTGTCAGTTCGGCCCCGGGCAGTGCTCGAAATCCTCACGTACGACGTG
TACGCTCCGGTTTCTCCGCG

Product: putative sensory kinase in two-component system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDD
PTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRHDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTMMIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLS
ERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAV
ELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYID
VINTGMPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK

Sequences:

>Translated_475_residues
MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDD
PTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRHDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTMMIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLS
ERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAV
ELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYID
VINTGMPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK
>Mature_475_residues
MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDD
PTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRHDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTMMIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLS
ERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAV
ELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYID
VINTGMPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK

Specific function: Probable member of a two-component regulatory system yfhA/yfhK. May activate yfhA by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=475, Percent_Identity=99.1578947368421, Blast_Score=966, Evalue=0.0,
Organism=Escherichia coli, GI1790861, Length=294, Percent_Identity=30.2721088435374, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI145693157, Length=228, Percent_Identity=29.8245614035088, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87081816, Length=223, Percent_Identity=31.390134529148, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=29.4871794871795, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789149, Length=354, Percent_Identity=24.2937853107345, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789808, Length=291, Percent_Identity=24.0549828178694, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=24.1758241758242, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788393, Length=279, Percent_Identity=24.0143369175627, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI48994928, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788549, Length=260, Percent_Identity=26.1538461538462, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790300, Length=232, Percent_Identity=31.4655172413793, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787894, Length=248, Percent_Identity=26.2096774193548, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=23.9864864864865, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788279, Length=331, Percent_Identity=23.8670694864048, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53322; Mature: 53322

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRHDLNN
HHHHHHHHHHHHHCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSL
HHEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHH
VLFLVSLVMVLLFTMMIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRN
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
LQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLA
HHHHHHHHHCCCHHHCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHH
EPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYIDVINTGMPIPQEERAMIFEPF
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHH
FQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK
HCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSEA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRHDLNN
HHHHHHHHHHHHHCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSL
HHEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHH
VLFLVSLVMVLLFTMMIIGPVKNIERMINRLGEGRSLGNSVSFSGPSELRSVGQRILWLS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
ERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSRN
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHH
LQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLA
HHHHHHHHHCCCHHHCCCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHH
EPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYIDVINTGMPIPQEERAMIFEPF
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCHHHHHHH
FQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESGQDVCFRIELPSSKNTK
HCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]