Click here to switch to the map view.

The map label for this gene is yfhA

Identifier: 218701067

GI number: 218701067

Start: 2861968

End: 2863302

Strand: Reverse

Name: yfhA

Synonym: ECIAI39_2758

Alternate gene names: 218701067

Gene position: 2863302-2861968 (Counterclockwise)

Preceding gene: 218701068

Following gene: 218701066

Centisome position: 55.79

GC content: 54.61

Gene sequence:

>1335_bases
ATGAGCCATAAACCTGCGCATTTATTATTGGTCGATGACGATCCGGGATTGCTGAAACTGCTTGGCCTGCGCCTGACCAG
CGAAGGCTACAGCGTGGTCACGGCGGAAAGTGGCGTTGAAGGATTACGGGTACTGAATCGCGAAAAAGTAGATTTAGTCA
TCAGCGACCTGCGGATGGATGAAATGGATGGTATGCAGCTATTTGCTGAAATTCAGAAAGTGCAGCCGGGAATGCCAGTA
ATTATTCTTACCGCGCATGGTTCTATTCCCGATGCCGTTGCAGCAACACAGCAGGGCGTTTTTAGTTTCCTCACCAAGCC
TGTCGATAAAGACGCGCTATATCAGGCAATTGACGATGCGCTGGAACAATCCGCGCCAGCCACCGACGAACGCTGGCGCG
AGGCGATTGTCACCCGCAGCCCGCTGATGCTGCGTTTGCTGGAACAGGCGCGGCTGGTGGCGCAATCAGACGTTAGCGTT
TTGATTAACGGCCAGAGCGGCACCGGGAAAGAGATTTTCGCCCAGGCTATCCACAACGCCAGCCCGCGCAACAGCAAACC
ATTTATTGCTATTAACTGTGGCGCATTGCCCGAGCAATTGCTGGAGTCGGAGCTGTTTGGTCATGCGCGTGGTGCGTTTA
CTGGCGCTGTCAGCAATCGCGAAGGTTTATTCCAGGCGGCGGAAGGCGGTACGCTATTTCTCGATGAGATTGGCGATATG
CCCGCACCGTTGCAGGTCAAACTGCTGCGCGTGTTGCAGGAGCGTAAAGTGCGCCCGCTGGGCAGTAACCGCGATATTGA
TATCGACGTGCGGATTATTTCTGCCACTCACCGCGACCTGCCAAAAGCGATGGCGCGCGGGGAATTCCGCGAAGATCTCT
ATTACCGCCTCAACGTTGTCAGCCTGAAAATTCCGGCACTGGCGGAGCGCACAGAAGACATTCCGCTACTGGCAAATCAC
CTGTTGCGCCAGGCGGCAGAGCGACATAAACCGTTTGTCCGCGCGTTCTCCACCGATGCGATGAAACGCCTGATGACCGC
GAGCTGGCCGGGTAATGTGCGCCAGTTGGTCAACGTGATTGAACAGTGCGTGGCGCTGACCTCATCTCCGGTGATTAGCG
ATGCATTAGTAGAGCAGGCGCTGGAGGGTGAAAATACGGCGCTGCCAACCTTTGTTGAGGCACGTAATCAGTTTGAACTC
AACTATTTGCGTAAGCTGCTGCAAATTACCAAAGGCAACGTCACCCACGCGGCGAGAATGGCGGGGCGCAACCGGACAGA
ATTTTATAAACTGCTATCCCGACACGAGCTGGATGCAAACGATTTCAAGGAATGA

Upstream 100 bases:

>100_bases
GAACGCCAGCTCTCGACCCGCAAACCGGCTGGAAATTTCTCGCCGGATACGCCGCACGAGAGTGAAAAGCCCGCGCCATC
CACCGATGAGGTCACTCCTG

Downstream 100 bases:

>100_bases
ATTGGCGTTATGTGTTACGTTTAGCAGATCAAAAGACAGGCGACCTTTTCAAGGAATAGCATGAAAAAGATTGATGCGAT
TATTAAACCCTTCAAGCTGG

Product: putative DNA-binding response regulator in two-component system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGVEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
PAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANH
LLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFEL
NYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Sequences:

>Translated_444_residues
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGVEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
PAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANH
LLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFEL
NYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE
>Mature_443_residues
SHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGVEGLRVLNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVI
ILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVL
INGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDMP
APLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHL
LRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFELN
YLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=444, Percent_Identity=99.5495495495496, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI1790437, Length=446, Percent_Identity=44.6188340807175, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=40.4395604395604, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=38.1465517241379, Blast_Score=300, Evalue=9e-83,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=54.7413793103448, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1789087, Length=307, Percent_Identity=46.9055374592834, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1789233, Length=252, Percent_Identity=48.8095238095238, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=40.1898734177215, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=381, Percent_Identity=38.3202099737533, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=37.5776397515528, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787583, Length=239, Percent_Identity=43.0962343096234, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=36.3636363636364, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=33.4394904458599, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=37.2727272727273, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49178; Mature: 49047

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGVEGLRVLNREKVDLVISDLRMD
CCCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHCCC
EMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDA
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVLINGQSGTGKEIFAQAIHNA
HHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
SPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEHHHHCCC
PAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNVV
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHHHHEEEEE
SLKIPALAERTEDIPLLANHLLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVI
EEECCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFELNYLRKLLQITKGNVTHAARM
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
AGRNRTEFYKLLSRHELDANDFKE
HCCCHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGVEGLRVLNREKVDLVISDLRMD
CCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHCCC
EMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYQAIDDA
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSVLINGQSGTGKEIFAQAIHNA
HHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCC
SPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
CCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCEEEHHHHCCC
PAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISATHRDLPKAMARGEFREDLYYRLNVV
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHHHHEEEEE
SLKIPALAERTEDIPLLANHLLRQAAERHKPFVRAFSTDAMKRLMTASWPGNVRQLVNVI
EEECCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQCVALTSSPVISDALVEQALEGENTALPTFVEARNQFELNYLRKLLQITKGNVTHAARM
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHH
AGRNRTEFYKLLSRHELDANDFKE
HCCCHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]