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The map label for this gene is nupG [H]

Identifier: 218700873

GI number: 218700873

Start: 2649373

End: 2650686

Strand: Reverse

Name: nupG [H]

Synonym: ECIAI39_2552

Alternate gene names: 218700873

Gene position: 2650686-2649373 (Counterclockwise)

Preceding gene: 218700876

Following gene: 218700872

Centisome position: 51.65

GC content: 42.09

Gene sequence:

>1314_bases
TTGGTAAATGTGATGAATTTCCGGTTATTTAAAGGGGGCTCAAAGTCGAAGGAGAAGATGGTGAATCTAAAACTACAATT
AAAAGTACTTTCATTTCTGCAATTCTTTTTGTGGGGAAGTTGGCTCAATACATTCGGTTCCTATATGTTTATCACGCTAC
GTTTTAAAGGCGAGGATATTGGTGCAGTCTATAGTACCTTGGGTATTGCAGCATTAATAACATCAACGTTATTTGGTATC
GTGGCCGATAAATGGATTAGTGCTAAATGGATATATGCCGCTTGTCATTTATTTGGCGCGATAACATTATTTGTCGCTGC
ATCGGTTACCACGCCTTCGGCAATGTTCATTGTCATTTTACTTAACTCTCTCGTGTATATGCCAACGCTCGGTTTGAGTA
ATACAATTTCCTATTACCGCATACAAACTGCGGGGATGGATATTGTGACAGAATTTCCACCAATCCGTATTTGGGGCACG
ATTGGATTTATTGTCGCGATGTGGGCGGTGAGTTTGGCAGGTTTTGAATTAAGTCATATGCAATTGTATATCGGCGCGGC
AGCTTCGTTAGGCTTGAGCCTGTTCACGTTGACATTACCGACTGTACCAGTGGCGAAATCTAACCAGGCGCAAGGAATGA
TAGACGTGCTCGGACTGCGTGCATTTGCACTGTTTAAGAACAGCAAAATGGCGATTTTCTTTATCTTTGCCATGCTGCTC
GGGGCAGAACTACAAATTACCGGGATGTTTGGTAATACATTTCTCCACAGTTTTGAGAGTGTTCCAGAGTTTGCCAATAG
CGTTGTTGTAACTCATGCTTCTATTTTGTTATCAATTTCTCAGATATCCGAAGTCTGCTTCATTCTTGCCATTCCCTTTT
TCCTGAAAAAATACGGTATCAAAAATGTCATGCTGATGAGCATGGTGGCCTGGACGCTGCGTTTTGGTTTATTCGCTTAT
GGCGATCCTTCATTCGGCGGTACCATTCTGTTAATTCTTTCGATGATTGTTTATGGCTGTGCTTTTGACTTCTTCAATAT
ATCTGGCTCGGTATTTGTTGAACAAGAAGTTGATCCATCGATTCGTAACAGCGCTCAGGGCGTTTTCCTGATGATGGCCA
ATGGTTTTGGTTGTATTCTGGGCGGTTTTATCAGTGGTAAAGTCGTCGATTATTTAACAACGGATGGCAATCCACAGTGG
TCAACCATTTGGCTGGTATTTGCCGGATATTCACTGGTTCTGGCTCTCGCATTTATGGTGTTGTTTAAATATAAACACAA
CAGTAACCCGACAACATCCGGTCATTTCGCCTGA

Upstream 100 bases:

>100_bases
ATCTCTAACCAATACATAATTATCACGTAATGATAGGGTGTCGATCTGAATGTCTTCAGGATTGATATCAAATTGCAATT
GATATCCGTGGTAAAAAGTA

Downstream 100 bases:

>100_bases
TACGGAATTTATCAAAATTAATAATGTTTTATAGGATTAGCAAAGGAGGATATTATGGCAAATCAATTGTATAACCAGTG
TCGGGTTTATTGTGCACCCT

Product: putative nucleoside transporter

Products: NA

Alternate protein names: Nucleoside-transport system protein nupG [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MVNVMNFRLFKGGSKSKEKMVNLKLQLKVLSFLQFFLWGSWLNTFGSYMFITLRFKGEDIGAVYSTLGIAALITSTLFGI
VADKWISAKWIYAACHLFGAITLFVAASVTTPSAMFIVILLNSLVYMPTLGLSNTISYYRIQTAGMDIVTEFPPIRIWGT
IGFIVAMWAVSLAGFELSHMQLYIGAAASLGLSLFTLTLPTVPVAKSNQAQGMIDVLGLRAFALFKNSKMAIFFIFAMLL
GAELQITGMFGNTFLHSFESVPEFANSVVVTHASILLSISQISEVCFILAIPFFLKKYGIKNVMLMSMVAWTLRFGLFAY
GDPSFGGTILLILSMIVYGCAFDFFNISGSVFVEQEVDPSIRNSAQGVFLMMANGFGCILGGFISGKVVDYLTTDGNPQW
STIWLVFAGYSLVLALAFMVLFKYKHNSNPTTSGHFA

Sequences:

>Translated_437_residues
MVNVMNFRLFKGGSKSKEKMVNLKLQLKVLSFLQFFLWGSWLNTFGSYMFITLRFKGEDIGAVYSTLGIAALITSTLFGI
VADKWISAKWIYAACHLFGAITLFVAASVTTPSAMFIVILLNSLVYMPTLGLSNTISYYRIQTAGMDIVTEFPPIRIWGT
IGFIVAMWAVSLAGFELSHMQLYIGAAASLGLSLFTLTLPTVPVAKSNQAQGMIDVLGLRAFALFKNSKMAIFFIFAMLL
GAELQITGMFGNTFLHSFESVPEFANSVVVTHASILLSISQISEVCFILAIPFFLKKYGIKNVMLMSMVAWTLRFGLFAY
GDPSFGGTILLILSMIVYGCAFDFFNISGSVFVEQEVDPSIRNSAQGVFLMMANGFGCILGGFISGKVVDYLTTDGNPQW
STIWLVFAGYSLVLALAFMVLFKYKHNSNPTTSGHFA
>Mature_437_residues
MVNVMNFRLFKGGSKSKEKMVNLKLQLKVLSFLQFFLWGSWLNTFGSYMFITLRFKGEDIGAVYSTLGIAALITSTLFGI
VADKWISAKWIYAACHLFGAITLFVAASVTTPSAMFIVILLNSLVYMPTLGLSNTISYYRIQTAGMDIVTEFPPIRIWGT
IGFIVAMWAVSLAGFELSHMQLYIGAAASLGLSLFTLTLPTVPVAKSNQAQGMIDVLGLRAFALFKNSKMAIFFIFAMLL
GAELQITGMFGNTFLHSFESVPEFANSVVVTHASILLSISQISEVCFILAIPFFLKKYGIKNVMLMSMVAWTLRFGLFAY
GDPSFGGTILLILSMIVYGCAFDFFNISGSVFVEQEVDPSIRNSAQGVFLMMANGFGCILGGFISGKVVDYLTTDGNPQW
STIWLVFAGYSLVLALAFMVLFKYKHNSNPTTSGHFA

Specific function: Transports nucleosides with a high affinity. Driven by a proton motive force [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli xapB and yegT [H]

Homologues:

Organism=Escherichia coli, GI87082192, Length=411, Percent_Identity=73.9659367396594, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI1788745, Length=417, Percent_Identity=61.6306954436451, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1788415, Length=417, Percent_Identity=29.0167865707434, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR004740 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 48006; Mature: 48006

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNVMNFRLFKGGSKSKEKMVNLKLQLKVLSFLQFFLWGSWLNTFGSYMFITLRFKGEDI
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCH
GAVYSTLGIAALITSTLFGIVADKWISAKWIYAACHLFGAITLFVAASVTTPSAMFIVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LNSLVYMPTLGLSNTISYYRIQTAGMDIVTEFPPIRIWGTIGFIVAMWAVSLAGFELSHM
HHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCHHHE
QLYIGAAASLGLSLFTLTLPTVPVAKSNQAQGMIDVLGLRAFALFKNSKMAIFFIFAMLL
EEEEEHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GAELQITGMFGNTFLHSFESVPEFANSVVVTHASILLSISQISEVCFILAIPFFLKKYGI
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KNVMLMSMVAWTLRFGLFAYGDPSFGGTILLILSMIVYGCAFDFFNISGSVFVEQEVDPS
HHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
IRNSAQGVFLMMANGFGCILGGFISGKVVDYLTTDGNPQWSTIWLVFAGYSLVLALAFMV
HHCCCCEEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHH
LFKYKHNSNPTTSGHFA
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MVNVMNFRLFKGGSKSKEKMVNLKLQLKVLSFLQFFLWGSWLNTFGSYMFITLRFKGEDI
CCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCH
GAVYSTLGIAALITSTLFGIVADKWISAKWIYAACHLFGAITLFVAASVTTPSAMFIVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LNSLVYMPTLGLSNTISYYRIQTAGMDIVTEFPPIRIWGTIGFIVAMWAVSLAGFELSHM
HHHHHHHCCCCCCCCCEEEEEEECCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCCHHHE
QLYIGAAASLGLSLFTLTLPTVPVAKSNQAQGMIDVLGLRAFALFKNSKMAIFFIFAMLL
EEEEEHHHHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GAELQITGMFGNTFLHSFESVPEFANSVVVTHASILLSISQISEVCFILAIPFFLKKYGI
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KNVMLMSMVAWTLRFGLFAYGDPSFGGTILLILSMIVYGCAFDFFNISGSVFVEQEVDPS
HHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCH
IRNSAQGVFLMMANGFGCILGGFISGKVVDYLTTDGNPQWSTIWLVFAGYSLVLALAFMV
HHCCCCEEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHH
LFKYKHNSNPTTSGHFA
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3311747; 9278503 [H]