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The map label for this gene is xapA

Identifier: 218700871

GI number: 218700871

Start: 2647327

End: 2648160

Strand: Reverse

Name: xapA

Synonym: ECIAI39_2550

Alternate gene names: 218700871

Gene position: 2648160-2647327 (Counterclockwise)

Preceding gene: 218700872

Following gene: 218700870

Centisome position: 51.6

GC content: 49.28

Gene sequence:

>834_bases
ATGACCCAACCTCATTTTTCACAAAACCCGTGGTATTGCGCAGATATCATTCGTCGACATAAACCAGATTTTACCCCACG
CGTGGTGTTTATTTTGGGATCGGGGCTGGGAGCGTTAGCTGAACAAATAGAAGATGCTGTTGCCATTTCTTATAGCGATT
TGCCGGGGTTTCCGGTGAGCACCGTACATGGCCATGCAGGAGAGTTGGTCCTCGGAGTGCTCTCTGGTGTGCCTGTAGCC
TGCATGAAAGGTCGCGGTCATTTTTATGAGGGCCGGGGAATGACAGTCATGACTGATGCTATTCGCACGTTCAAGTTGTT
GGGCTGTGAATTGTTGTTCTGCACCAACGCTGCAGGTTCGCTTCGTCCAGAAGTCGGTGCGGGTAGTCTGGTTGCGCTGA
AAGATCACATTAATACGATGCCAGGCACACCGATGACAGGCCTTAATGATGAGCGTTTTGGCGAACGCTTTTTTTCATTA
GCGAATGCTTACGATGCTGATTACCGCGCCGTTTTACAAACCGTCGCGCAGGAAGAAGGTTTTCCGCTGACAGAAGGGGT
TTTTGTTTCTTATCCGGGCCCGAACTTCGAAACGGCGGCAGAAATTCGCATGATGCAGCTGATTGGTGGCGATGTTGTGG
GTATGTCTGTAGTGCCTGAAGTGATTAGCGCACGTCATTGCGAAATGAAAGTCGTTGCCGTGTCCGCAATAACCAATATG
GCAGAAGGCTTAAGTGATGTGAAATTGTCGCATGCCCAAACCCTGGCTGCAGCAGAGTTATCACGGCAAAACTTTATTAA
TCTGATTTGCGGATTCTTACGCAAAATCGCCTGA

Upstream 100 bases:

>100_bases
TGAATATCACAGGAAAATGTATTGGTCACTCGCGACTTTTTCTCGTTATAAAAAGATGCGACTCATATCTTACGAATAAT
GCATATTATAAGGACTTTCC

Downstream 100 bases:

>100_bases
AAACAATAAAAATGGCCTCGCGGATGGGGCCATCTCACTGCGATAAGGAAATAAAAAATGAATATCGCAACGCGTTTAAA
GGTAATGTCTTTCTTACAAT

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTQPHFSQNPWYCADIIRRHKPDFTPRVVFILGSGLGALAEQIEDAVAISYSDLPGFPVSTVHGHAGELVLGVLSGVPVA
CMKGRGHFYEGRGMTVMTDAIRTFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMTGLNDERFGERFFSL
ANAYDADYRAVLQTVAQEEGFPLTEGVFVSYPGPNFETAAEIRMMQLIGGDVVGMSVVPEVISARHCEMKVVAVSAITNM
AEGLSDVKLSHAQTLAAAELSRQNFINLICGFLRKIA

Sequences:

>Translated_277_residues
MTQPHFSQNPWYCADIIRRHKPDFTPRVVFILGSGLGALAEQIEDAVAISYSDLPGFPVSTVHGHAGELVLGVLSGVPVA
CMKGRGHFYEGRGMTVMTDAIRTFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMTGLNDERFGERFFSL
ANAYDADYRAVLQTVAQEEGFPLTEGVFVSYPGPNFETAAEIRMMQLIGGDVVGMSVVPEVISARHCEMKVVAVSAITNM
AEGLSDVKLSHAQTLAAAELSRQNFINLICGFLRKIA
>Mature_276_residues
TQPHFSQNPWYCADIIRRHKPDFTPRVVFILGSGLGALAEQIEDAVAISYSDLPGFPVSTVHGHAGELVLGVLSGVPVAC
MKGRGHFYEGRGMTVMTDAIRTFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMTGLNDERFGERFFSLA
NAYDADYRAVLQTVAQEEGFPLTEGVFVSYPGPNFETAAEIRMMQLIGGDVVGMSVVPEVISARHCEMKVVAVSAITNMA
EGLSDVKLSHAQTLAAAELSRQNFINLICGFLRKIA

Specific function: The nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the nucleoside molecule, with the formation of the corresponding free bases and pentose-1-phosphate. This protein can degrade all purine nucleosides except ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=259, Percent_Identity=43.6293436293436, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1788746, Length=277, Percent_Identity=89.5306859205776, Blast_Score=519, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17541190, Length=254, Percent_Identity=35.8267716535433, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323238, Length=290, Percent_Identity=37.5862068965517, Blast_Score=172, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24656090, Length=285, Percent_Identity=40.7017543859649, Blast_Score=201, Evalue=3e-52,
Organism=Drosophila melanogaster, GI45552887, Length=260, Percent_Identity=42.3076923076923, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI24656093, Length=260, Percent_Identity=42.3076923076923, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI45552885, Length=260, Percent_Identity=42.3076923076923, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24762376, Length=221, Percent_Identity=33.0316742081448, Blast_Score=111, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR010943
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.-

Molecular weight: Translated: 29839; Mature: 29708

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPHFSQNPWYCADIIRRHKPDFTPRVVFILGSGLGALAEQIEDAVAISYSDLPGFPVS
CCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCH
TVHGHAGELVLGVLSGVPVACMKGRGHFYEGRGMTVMTDAIRTFKLLGCELLFCTNAAGS
HCCCCHHHHHHHHHCCCCEEEECCCCCEECCCCCEEHHHHHHHHHHHHHEEEEEECCCCC
LRPEVGAGSLVALKDHINTMPGTPMTGLNDERFGERFFSLANAYDADYRAVLQTVAQEEG
CCCCCCCCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
FPLTEGVFVSYPGPNFETAAEIRMMQLIGGDVVGMSVVPEVISARHCEMKVVAVSAITNM
CCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
AEGLSDVKLSHAQTLAAAELSRQNFINLICGFLRKIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQPHFSQNPWYCADIIRRHKPDFTPRVVFILGSGLGALAEQIEDAVAISYSDLPGFPVS
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCH
TVHGHAGELVLGVLSGVPVACMKGRGHFYEGRGMTVMTDAIRTFKLLGCELLFCTNAAGS
HCCCCHHHHHHHHHCCCCEEEECCCCCEECCCCCEEHHHHHHHHHHHHHEEEEEECCCCC
LRPEVGAGSLVALKDHINTMPGTPMTGLNDERFGERFFSLANAYDADYRAVLQTVAQEEG
CCCCCCCCCEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
FPLTEGVFVSYPGPNFETAAEIRMMQLIGGDVVGMSVVPEVISARHCEMKVVAVSAITNM
CCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
AEGLSDVKLSHAQTLAAAELSRQNFINLICGFLRKIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559336; 9205837; 9278503 [H]