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The map label for this gene is ypdF

Identifier: 218700853

GI number: 218700853

Start: 2627271

End: 2628356

Strand: Reverse

Name: ypdF

Synonym: ECIAI39_2530

Alternate gene names: 218700853

Gene position: 2628356-2627271 (Counterclockwise)

Preceding gene: 218700854

Following gene: 218700852

Centisome position: 51.21

GC content: 57.73

Gene sequence:

>1086_bases
ATGACATTACTCGCTTCGCTGCGCGACTGGCTTAAGGCGCAACAACTGGATGCGGTGCTTCTCTCCTCACGGCAGAACAA
ACAGCCGCATCTGGGGATCTCCACCGGATCAGGCTATGTGGTGATTAGCCGTGAAAGTGCGCACATTCTGGTGGACTCGC
GCTATTATGCGGATGTAGAAGCCCGCACGCAAGGTTACCAGCTGCATTTGCTTGACGCGACGCACACGCTTGCAACCATC
GCCAGGCAAATCATTGCCGATGAGCAGTTAAAAACGCTCGGTTTTGAAGGCCAGCAGGTGAGTTGGGAAACCGCGCACCG
CTGGCAGTCTGAACTCAATGCGAAACTGGTAAGCGCCACGCCGGATGTGCTGCGGCAAATCAAAACGCCAGAGGAGGTGG
AGAAAATCCGCCTCGCCTGTGGGATTGCCGATCGCGGTGCTGAGCATATTCGCCGCTTTATTCAGGCGGGGATGAGCGAG
CGCGAGATAGCCGCTGAACTGGAGTGGTTTATGCGCCAGCAGGGCGCAGAAAAAGCCTCTTTCGATACCATTGTCGCCAG
CGGCTGGCGTGGGGCGCTGCCGCACGGCAAAGCCAGCGACAAGATTGTTACAGCGGGCGAGTTTGTCACTCTCGATTTCG
GTGCGCTCTATCAGGGCTACTGCTCTGATATGACACGCACCTTGCTGGTGAATGGCGAAGGGGTGAGCGCCGAATCTCAC
CCGCTGTTTAACGTCTATCAGATTGTTTTGCAGGCACAGCTGGCGGCAATCTCCGCGATTCGCCCCGGCGTGCGCTGCCA
GCAGGTTGACGACGCCGCGCGCCGGGTGATTACCGAGGCTGGATTTGGCGACTATTTCGGTCATACCACCGGTCATGCTA
TCGGCATTGAAGTCCATGAAGATCCGCGTTTTTCACCGCGGGACACCACGACGCTACAGCCAGGCATGTTACTGACCGTG
GAGCCGGGGATTTATTTGCCAGGGCAAGGGGGCGTGCGCATCGAAGATGTTGTGCTGGTCACCCCGCAAGGCGCAGAAGT
GCTCTACGCCATGCCGAAAACAGTGTTGCTCACGGGAGAGGCATAA

Upstream 100 bases:

>100_bases
TGGGGGCAGTCATTACTGCGCTGATGGTGGTGTTCCTGCGTCTGATGATGTTCCGTAAAGGCAAATTGTTAATCGATAGC
CTGTAAGAAGGAAAATCCAG

Downstream 100 bases:

>100_bases
TGGATTTATCGCTATTAAAAGCGTTGAGCGAGGCAGATGCGATCGCCTCCTCGGAACAGGAAGTACGGCAGATCCTGCTG
GAAGAAGCGGATCGCCTGCA

Product: aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYADVEARTQGYQLHLLDATHTLATI
ARQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
PLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHTTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTV
EPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA

Sequences:

>Translated_361_residues
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYADVEARTQGYQLHLLDATHTLATI
ARQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
PLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHTTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTV
EPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA
>Mature_360_residues
TLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIA
RQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSER
EIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESHP
LFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHTTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVE
PGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA

Specific function: Hydrolyzes the N-terminal methionine when the next amino acid is alanine, proline or serine. The substrate preference for methionyl aminopeptidase activity is Pro > Ala > Ser. Also able to hydrolyze the Xaa-Pro peptide bond when the first amino acid is al

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24 family [H]

Homologues:

Organism=Homo sapiens, GI11559925, Length=254, Percent_Identity=30.3149606299213, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI149589008, Length=307, Percent_Identity=27.6872964169381, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI260593665, Length=307, Percent_Identity=27.6872964169381, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI260593663, Length=242, Percent_Identity=29.3388429752066, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI264681563, Length=198, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI93141226, Length=174, Percent_Identity=34.4827586206897, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI264681565, Length=195, Percent_Identity=31.7948717948718, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788728, Length=361, Percent_Identity=96.6759002770083, Blast_Score=717, Evalue=0.0,
Organism=Escherichia coli, GI1789275, Length=254, Percent_Identity=29.9212598425197, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786364, Length=207, Percent_Identity=25.6038647342995, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790282, Length=300, Percent_Identity=26, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17508215, Length=312, Percent_Identity=28.2051282051282, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71989583, Length=250, Percent_Identity=25.2, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6321118, Length=285, Percent_Identity=27.3684210526316, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320922, Length=240, Percent_Identity=24.1666666666667, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323273, Length=253, Percent_Identity=24.901185770751, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19920384, Length=260, Percent_Identity=27.6923076923077, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357079, Length=294, Percent_Identity=27.891156462585, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137632, Length=172, Percent_Identity=33.7209302325581, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 39604; Mature: 39473

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYADVE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEECCEEECCH
ARTQGYQLHLLDATHTLATIARQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSAT
HCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCC
PDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSEREIAAELEWFMRQQGAEKAS
HHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHH
FDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
HHHHHCCCCCCCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHCEEEEEECCCCCCCCCC
PLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHTTGHAIGIEVHE
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEECC
DPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGE
CCCCCCCCCCEECCCEEEEECCCEEECCCCCCEEEEEEEECCCCCEEEEECCCEEEEECC
A
C
>Mature Secondary Structure 
TLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISRESAHILVDSRYYADVE
CHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCEEEEECCEEECCH
ARTQGYQLHLLDATHTLATIARQIIADEQLKTLGFEGQQVSWETAHRWQSELNAKLVSAT
HCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEECCC
PDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSEREIAAELEWFMRQQGAEKAS
HHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHH
FDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYQGYCSDMTRTLLVNGEGVSAESH
HHHHHCCCCCCCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHCEEEEEECCCCCCCCCC
PLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRVITEAGFGDYFGHTTGHAIGIEVHE
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEECC
DPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGE
CCCCCCCCCCEECCCEEEEECCCEEECCCCCCEEEEEEEECCCCCEEEEECCCEEEEECC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]