Click here to switch to the map view.

The map label for this gene is menD

Identifier: 218700739

GI number: 218700739

Start: 2496603

End: 2498273

Strand: Reverse

Name: menD

Synonym: ECIAI39_2412

Alternate gene names: 218700739

Gene position: 2498273-2496603 (Counterclockwise)

Preceding gene: 218700740

Following gene: 218700738

Centisome position: 48.68

GC content: 57.93

Gene sequence:

>1671_bases
ATGTCAGTAAGCGCATTTAACCGACGCTGGGCGGCGGTCATTCTGGAAGCATTAACGCGTCACGGCGTCAGACACATCTG
TATCGCCCCTGGCTCGCGTTCTACACCGTTAACGTTAGCGGCGGCGGAGAATTCCGCATTCATTCACCACACCCATTTCG
ATGAGCGTGGACTGGGGCATCTGGCGCTGGGGCTGGCGAAAGTCAGCAAGCAGCCGGTGGCTGTGATTGTGACCTCCGGC
ACGGCGGTAGCAAATCTCTATCCGGCACTGATTGAAGCCGGGTTAACCGGAGAAAAACTGATCCTGTTAACCGCCGATCG
CCCGCCGGAGCTCATTGACTGCGGCGCGAATCAGGCGATTCGTCAGCCGGGAATGTTCGCCTCTCACCCCACGCACAGTA
TTTCATTGCCGCGCCCGACCCAGGATATCCCCGCACGTTGGCTGGTTTCTACCATCGACCACGCTCTCGGTACGCTTCAT
GCTGGTGGAGTCCATATCAACTGTCCGTTTGCCGAACCGCTGTATGGCGAAATGGACGACACCGGGCTTAGCTGGCAACA
GCGTCTGGGCGACTGGTGGCAGGACGATAAGCCGTGGCTGCGTGAAGCGCCTCGTCTGGAAAGTGAAAAACAGCGCGACT
GGTTCTTCTGGCGACAAAAGCGCGGCGTGGTGGTTGCCGGGCGCATGAGTGCGGAAGAGGGCAAAAAAGTCGCACTGTGG
GCGCAAACTCTTGGCTGGCCGCTGATTGGCGACGTGCTGTCGCAAACCGGACAGCCGCTGCCGTGTGCCGATCTCTGGTT
AGGCAATGCCAAAGCGACCAGCGAACTGCAACAAACGCAAATTGTGGTGCAACTGGGAAGCAGCCTGACGGGCAAACGGC
TCCTGCAATGGCAGGCAAGCTGTGAACCGGAAGAGTACTGGATTGTTGATGACATCGAAGGGCGACTTGATCCGGCACAC
CATCGCGGACGTCGCTTAATTGCCAATATTGCCGACTGGCTGGAGCTGCATCCGGCAGAAAAACGCCAGCCCTGGTGCGT
TGAAATCCCGCGCCTGGCGGAACAGGCAATGCAGGCGGTTATTGCCCGTCGCGATGCGTTTGGCGAAGCCCAACTGGCGC
ATCGCATCAGCGACTATCTGCCTGAACAGGGGCAATTGTTTGTCGGTAACAGCCTGGTGGTACGTCTGATTGATGCGCTT
TCGCAACTTCCGGCAGGTTACCCGGTGTACAGCAACCGTGGTGCCAGCGGTATCGACGGGCTGCTCTCAACTGCCGCTGG
CGTTCAGCGGGCCAGTGGCAAACCGACGCTGGCGATTGTGGGCGATCTCTCAGCACTTTACGATCTCAACGCGCTGGCGT
TATTACGTCAGGTTTCCGCGCCGCTGGTATTAATTGTGGTGAACAACAACGGCGGGCAAATTTTCTCGCTGTTGCCAACG
CCGAAAAACGAGCGCGAGCGTTTCTATCTGATGCCGCAAAACGTCCATTTTGAGCACGCCGCCGCGATGTTCGAGCTGAA
ATATCATCGTCCGCAAAACTGGCAGGAACTTGAAACGGCACTTGTCGACGCCTGGCGCACGCCGACCACCACGGTAATTG
AAATGGTGGTTAACGACACCGATGGTGCGCAAACCCTCCAGCAACTGCTGGCGCAGGTAAGCCATTTATGA

Upstream 100 bases:

>100_bases
CAAATGGAATAGTAATGAGTCGCATCATTACCGATTCATATCAATAATCTATTTTTGTAGCTCCTTATACTTAGCCGCAA
TATTGATACCGGACAAACTC

Downstream 100 bases:

>100_bases
TCCTGCACGCGCAGGCAAAACACGGTAAACCAGGTTTACCCTGGCTGGTGTTTTTGCACGGTTTTTCCGGCGATTGCCAT
GAATGGCAAGAAGTGGGCGA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSG
TAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW
AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQTQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAH
HRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDAL
SQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT
PKNERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETALVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL

Sequences:

>Translated_556_residues
MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSG
TAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW
AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQTQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAH
HRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDAL
SQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT
PKNERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETALVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL
>Mature_555_residues
SVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGT
AVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLHA
GGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALWA
QTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQTQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHH
RGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDALS
QLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPTP
KNERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETALVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=556, Percent_Identity=98.9208633093525, Blast_Score=1124, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 61403; Mature: 61272

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGH
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCHH
LALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAI
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH
RQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLHAGGVHINCPFAEPLYGEMDD
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEECCCEEEECCCCCCCCCCCCC
TGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW
CCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCHHHEECCCCEEEEECCCCCCCCEEEEE
AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQTQIVVQLGSSLTGKRLLQWQAS
HHHCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
CEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAV
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH
IARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDALSQLPAGYPVYSNRGASGIDG
HHHHHCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCC
PKNERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETALVDAWRTPTTTVIEMVVNDT
CCCCCCEEEECCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHEEEEEECCC
DGAQTLQQLLAQVSHL
CHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLGH
CCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCCCCCHH
LALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAI
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEECCCCHHH
RQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLHAGGVHINCPFAEPLYGEMDD
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEECCCEEEECCCCCCCCCCCCC
TGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQKRGVVVAGRMSAEEGKKVALW
CCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCHHHEECCCCEEEEECCCCCCCCEEEEE
AQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQTQIVVQLGSSLTGKRLLQWQAS
HHHCCCHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
CEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAV
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH
IARRDAFGEAQLAHRISDYLPEQGQLFVGNSLVVRLIDALSQLPAGYPVYSNRGASGIDG
HHHHHCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
LLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVSAPLVLIVVNNNGGQIFSLLPT
HHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCC
PKNERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETALVDAWRTPTTTVIEMVVNDT
CCCCCCEEEECCCCCCHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHEEEEEECCC
DGAQTLQQLLAQVSHL
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA