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The map label for this gene is ycaJ

Identifier: 218700590

GI number: 218700590

Start: 2316287

End: 2317630

Strand: Reverse

Name: ycaJ

Synonym: ECIAI39_2256

Alternate gene names: 218700590

Gene position: 2317630-2316287 (Counterclockwise)

Preceding gene: 218700591

Following gene: 218700589

Centisome position: 45.16

GC content: 53.12

Gene sequence:

>1344_bases
GTGAGCAATCTGTCGCTCGATTTTTCGGATAATACTTTTCAACCTCTGGCCGCGCGTATGCGGCCAGAAAATTTAGCACA
GTATATCGGCCAGCAACATTTGCTGGCTGCGGGGAAGCCGTTGCCGCGCGCTATCGAAGCCGGGCATTTGCATTCTATGA
TCCTCTGGGGGCCACCGGGTACCGGCAAAACAACCCTCGCTGAAGTGATTGCCCGCTATGCGAACGCTGATGTGGAACGT
ATTTCTGCTGTCACCTCTGGCGTGAAAGAGATTCGCGAGGCGATTGAGCGCGCTCGGCAAAACCGCAATGCAGGTCGCCG
CACTATTCTTTTTGTTGACGAAGTTCACCGTTTCAACAAAAGCCAGCAGGATGCATTTCTGCCACATATTGAAGACGGCA
CCATCACTTTTATTGGCGCAACCACTGAAAACCCGTCATTTGAGCTTAATTCGGCACTGCTTTCCCGTGCCCGTGTCTAT
CTGCTGAAATCCCTCAGTACAAAGGATATTGAGCAAGTACTAACTCAGGCGATGGAAGACAAGACCCGTGGCTATGGTGG
TCAGGATATTGTTCTGCCAGATGAAACACGACGCGCCATTGCTGAACTGGTGAATGGCGACGCGCGCCGGGCGTTAAATA
CGCTGGAAATGATGGCGGATATGGCCGAAGTCGATGATAGCGGTAAGCGGGTCCTGAAACCTGAATTACTGACCGAAATC
GCCGGTGAACGTAGCGCCCGCTTTGATAACAAAGGCGATCGCTTTTACGATCTCATTTCCGCACTGCATAAGTCGGTACG
TGGTAGCGCACCCGATGCGGCGCTGTACTGGTATGCGCGAATTATTACTGCCGGAGGCGATCCGTTATATGTCGCGCGTC
GCTGTCTGGCGATAGCGTCGGAAGATGTGGGTAATGCTGATCCACGAGCGATGCAGGTGGCAATTGCGGCCTGGGATTGC
TTTACTCGCGTTGGCCCGGCGGAAGGTGAACGCGCCATTGCTCAGGCGATTGTTTACCTCGCCTGCGCGCCAAAAAGCAA
CGCTGTCTACACCGCGTTTAAAGCCGCGCTGGCCGATGCTCGCGAACGTCCGGATTATGACGTGCCGGTTCATTTGCGTA
ATGCGCCGACGAAATTAATGAAGGAAATGGGCTACGGGCAGGAATATCGTTACGCTCATGATGAAGCAAACGCTTATGCT
GCCGGTGAGGTTTATTTCCCGCCGGAAATAGCACAAACACGCTATTATTTCCCGACAAACAGGGGCCTTGAAGGCAAGAT
TGGCGAAAAGCTCGCCTGGCTGGCTGAACAGGATCAAAATAGCCCCATAAAACGCTACCGTTAA

Upstream 100 bases:

>100_bases
CTGAAATCCCAGCAAAATGGGGCTGTGGATGCAGCGAAATTTACCTTCACCCCGCCGCAAGGCGTCACGGTAGATGATCA
ACGTAAGTAGAGGCACCTGA

Downstream 100 bases:

>100_bases
TGTTATCGTTGCGGTAATGTTGTTACTGTATCCCTGTGGTCGCAGGCTGTGGCCACATCTCCCATTTAATTCGATAAGCA
CAGGATAAGCATGCTCGATC

Product: recombination factor protein RarA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVER
ISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
LLKSLSTKDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSGKRVLKPELLTEI
AGERSARFDNKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDC
FTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYAHDEANAYA
AGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR

Sequences:

>Translated_447_residues
MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVER
ISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
LLKSLSTKDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSGKRVLKPELLTEI
AGERSARFDNKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDC
FTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYAHDEANAYA
AGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR
>Mature_446_residues
SNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERI
SAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVYL
LKSLSTKDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSGKRVLKPELLTEIA
GERSARFDNKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCF
TRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYAHDEANAYAA
GEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR

Specific function: Unknown

COG id: COG2256

COG function: function code L; ATPase related to the helicase subunit of the Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rarA family [H]

Homologues:

Organism=Homo sapiens, GI18426902, Length=438, Percent_Identity=40.8675799086758, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI18426904, Length=438, Percent_Identity=36.3013698630137, Blast_Score=241, Evalue=9e-64,
Organism=Escherichia coli, GI1787119, Length=447, Percent_Identity=99.7762863534676, Blast_Score=920, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324111, Length=448, Percent_Identity=40.625, Blast_Score=305, Evalue=9e-84,
Organism=Saccharomyces cerevisiae, GI6324039, Length=237, Percent_Identity=26.1603375527426, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR021886 [H]

Pfam domain/function: PF00004 AAA; PF12002 MgsA_C [H]

EC number: NA

Molecular weight: Translated: 49626; Mature: 49494

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPG
CCCCEECCCCCCHHHHHHHCCHHHHHHHHCHHHHHHCCCCCCHHHHCCCCCEEEEECCCC
TGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFNK
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCC
SQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTKDIEQVLTQAMED
CCCCCCCCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSGKRVLKPELLTEI
HHCCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHH
AGERSARFDNKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIAS
HCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADA
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
RERPDYDVPVHLRNAPTKLMKEMGYGQEYRYAHDEANAYAAGEVYFPPEIAQTRYYFPTN
HHCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCCCCCCEECCEEECCCHHHCCEEECCCC
RGLEGKIGEKLAWLAEQDQNSPIKRYR
CCCCCCHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
SNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPG
CCCEECCCCCCHHHHHHHCCHHHHHHHHCHHHHHHCCCCCCHHHHCCCCCEEEEECCCC
TGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFNK
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCC
SQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTKDIEQVLTQAMED
CCCCCCCCEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSGKRVLKPELLTEI
HHCCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHH
AGERSARFDNKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIAS
HCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
EDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADA
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHH
RERPDYDVPVHLRNAPTKLMKEMGYGQEYRYAHDEANAYAAGEVYFPPEIAQTRYYFPTN
HHCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCCCCCCEECCEEECCCHHHCCEEECCCC
RGLEGKIGEKLAWLAEQDQNSPIKRYR
CCCCCCHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]