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The map label for this gene is hyaF

Identifier: 218700503

GI number: 218700503

Start: 2216901

End: 2217758

Strand: Reverse

Name: hyaF

Synonym: ECIAI39_2169

Alternate gene names: 218700503

Gene position: 2217758-2216901 (Counterclockwise)

Preceding gene: 218700504

Following gene: 218700502

Centisome position: 43.21

GC content: 55.24

Gene sequence:

>858_bases
ATGAGCGAAACTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGAT
CACCTGTCAGGTGAATGATGAACCGAGTATGGCGGCCCTGGAGCAATGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAA
ACGAGTTACAACATCAACTAAGCGAACGCCAGCCGCCGTTGGGCGAGGTGCTGGCAGTCGATCTGTTAAATCTCAACGCC
GACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATACAGCAGGCTGACGACAGTGAAAG
TGAAATACAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCGCGGCGACAAGTTGCTGGAGGACAAACTGG
AGGCTGGCTGCGCGCCGCTGGCATTGTGGCAGGCGGCAACGCAAAACGTCTTGCCGACAGATTCGCTGTTACCGCCGCCC
ATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCGCATGTACGTAACCCCGACGCGCAGCCGCACAG
CATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGA
TTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACC
TTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGA
CTCTGCGCAGCGGCTTAGCGAGGTATGTCAGTGGCTGGCGGAAGGTGCACCGACGTAA

Upstream 100 bases:

>100_bases
CCGGCGGAAACTATCGCGGCGTGCTGAATGGTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGTGGGCTTGTCGAA
CCACAACAGGAGCGTGCCTC

Downstream 100 bases:

>100_bases
AAAGACGGTAAGTATCGCTTTCAGTCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGA
GGGAGATCTACCATTATCGG

Product: hydrogenase-1 nickel incorportation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNA
DDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT

Sequences:

>Translated_285_residues
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNA
DDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT
>Mature_284_residues
SETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNAD
DRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPPI
DGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTL
KGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT

Specific function: Not known. Could enhance the incorporation of nickel to the hydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family [H]

Homologues:

Organism=Escherichia coli, GI1787211, Length=285, Percent_Identity=98.9473684210526, Blast_Score=575, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006894 [H]

Pfam domain/function: PF04809 HupH_C [H]

EC number: NA

Molecular weight: Translated: 31389; Mature: 31258

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL
CCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
HHHHCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHEEEEEECHH
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT
HCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCAHSPQVIALLNELQHQL
CCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQADDSESEIQEAIFCGLWRV
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
HHHHCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
CCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHEEEEEECHH
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT
HCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]