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The map label for this gene is flgF

Identifier: 218700420

GI number: 218700420

Start: 2132921

End: 2133676

Strand: Reverse

Name: flgF

Synonym: ECIAI39_2086

Alternate gene names: 218700420

Gene position: 2133676-2132921 (Counterclockwise)

Preceding gene: 218700421

Following gene: 218700419

Centisome position: 41.58

GC content: 59.66

Gene sequence:

>756_bases
ATGGATCACGCAATTTATACCGCGATGGGAGCAGCCAGCCAGACGCTGAATCAACAGGCGGTAACCGCCAGTAATCTGGC
CAATGCCTCTACGCCCGGTTTTCGCGCGCAGTTAAACGCCTTGCGCGCAGTGCCGGTGGAAGGGCTTTCACTGCCCACGC
GCACGCTGGTAACGGCGTCAACGCCGGGTGCAGACATGACGCCCGGCAAGATGGATTACACCTCGCGCCCGCTGGACGTT
GCGTTGCAGCAGGATGGCTGGCTGGCCGTGCAGACCGCTGACAGCAGCGAAGGGTATACGCGTAATGGCAGCATTCAGGT
TGATCCCACCGGGCAACTGACAATTCAGGGACATCCGGTGATAGGCGAGGCGGGGCCAATTGCTGTGCCGGAAGGGGCGG
AAATCACTATTGCTGCCGATGGCACAATCTCGGCGCTGAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTGGGGCGT
CTGAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCGGAAAGCCAGGC
CACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGAGTTCTGGAAGGCAGTAACGTCAATGCCG
TTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAAC
GCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAA

Upstream 100 bases:

>100_bases
GCCCAGCGTAACTATCAGTCTAACGCCCAGACCATCAAAACCCAGGACCAGATCCTCAATACGCTGGTTAACTTACGCTA
ATCGCTGACGGGATAGCTCA

Downstream 100 bases:

>100_bases
TTAAACGATTGATGACATTGCAATAAAAGCGCGCGGACTATTCTCTCGCCCATTTTGGGAGAGGTTAGGGTGAGGGGAAA
TTCGCACCGTCGCGTGCTTC

Product: flagellar basal body rod protein FlgF

Products: NA

Alternate protein names: Putative proximal rod protein [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAVPVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDV
ALQQDGWLAVQTADSSEGYTRNGSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMSDMIASARRFEMQMKVISSVDDN
AGRANQLLSMS

Sequences:

>Translated_251_residues
MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAVPVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDV
ALQQDGWLAVQTADSSEGYTRNGSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMSDMIASARRFEMQMKVISSVDDN
AGRANQLLSMS
>Mature_251_residues
MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAVPVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDV
ALQQDGWLAVQTADSSEGYTRNGSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMSDMIASARRFEMQMKVISSVDDN
AGRANQLLSMS

Specific function: Unknown

COG id: COG4787

COG function: function code N; Flagellar basal body rod protein

Gene ontology:

Cell location: Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flagella basal body rod proteins family [H]

Homologues:

Organism=Escherichia coli, GI1787317, Length=251, Percent_Identity=99.203187250996, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1787318, Length=250, Percent_Identity=31.2, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010930
- InterPro:   IPR001444
- InterPro:   IPR019776
- InterPro:   IPR020013
- InterPro:   IPR012836 [H]

Pfam domain/function: PF06429 DUF1078; PF00460 Flg_bb_rod [H]

EC number: NA

Molecular weight: Translated: 25928; Mature: 25928

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00588 FLAGELLA_BB_ROD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAVPVEGLSLPTRTLVTAS
CCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEC
TPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADSSEGYTRNGSIQVDPTGQLTIQGHPV
CCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCEEECCCEEECCCCEEEECCCCE
IGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGRLKLVKATGSEVQRGDDGIFR
ECCCCCEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCCHHHCCCCCEEE
LSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMSDMIASARRFEMQMKVISSVDDN
EECCCCCCCCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGRANQLLSMS
CCCCCCEEECC
>Mature Secondary Structure
MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAVPVEGLSLPTRTLVTAS
CCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEC
TPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADSSEGYTRNGSIQVDPTGQLTIQGHPV
CCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCEEECCCEEECCCCEEEECCCCE
IGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGRLKLVKATGSEVQRGDDGIFR
ECCCCCEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCCHHHCCCCCEEE
LSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMSDMIASARRFEMQMKVISSVDDN
EECCCCCCCCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGRANQLLSMS
CCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]