Click here to switch to the map view.

The map label for this gene is flgK

Identifier: 218700416

GI number: 218700416

Start: 2127492

End: 2129135

Strand: Reverse

Name: flgK

Synonym: ECIAI39_2080

Alternate gene names: 218700416

Gene position: 2129135-2127492 (Counterclockwise)

Preceding gene: 218700417

Following gene: 218700415

Centisome position: 41.49

GC content: 52.01

Gene sequence:

>1644_bases
ATGTCCAGCTTGATCAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAACAATATCTC
CAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTGGG
TTGGCAATGGCGTCTACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCTGCGCAGACG
CAAAGTAGCGGCCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGACAATATGCTCTCCACCAGTACCTCTTCGCTGGC
AACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGTGAGTAACGCGGAAGACCCGGCAGCGCGCCAGGCGCTGA
TTGGGAAATCAGAAGGGTTGGTGAATCAGTTTAAAACCACCGATCAATATCTGCGCGACCAGGACAAACAGGTCAATATC
GCGATAGGTGCCAGCGTTGATCAGATCAACAATTACGCTAAACAAATCGCCAGCCTGAACGATCAAATCTCGCGTCTGAC
AGGCGTGGGGGCAGGGGCGTCACCTAACAATCTGCTGGATCAACGCGATCAACTGGTGAGTGAATTAAACCAGATTGTTG
GCGTAGAAGTTAGCGTTCAGGATGGTGGCACTTATAACATCACGATGGCCAATGGCTACTCACTGGTTCAGGGAAGTACG
GCGCGGCAGCTGGCGGCAGTTCCTTCCAGCGCCGACCCTTCTCGTACGACTGTCGCTTATATTGATGGGACGGCTGGCAA
TATTGAGATCCCGGAGAAATTACTGAATACCGGGTCGCTGGGCGGCATTCTGACATTCCGTTCTCAGGATCTGGACCAGA
CGCGTAATACGCTCGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAAC
GGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAGGTGACGTTGCGAT
CGGTGCAACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCA
CCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGATGCCAACGGTAAAGTGGCATTTGATGGTCTGGAGTTGACG
TTTACAGGAACGCCTGCCGTTAACGACAGCTTCACGCTGAAACCGGTAAGTGACGCCATCGTCAACATGGATGTATTAAT
CACCGACGAAGCGAAAATCGCCATGGCGAGCGCAGAAGATGCGGGTGATAGCGACAACCGCAACGGTCAGGCCCTGCTCG
ATCTGCAAAGCAACAGTAAAACGGTGGGCGGTGCGAAATCCTTTAACGACGCTTATGCCTCGTTAGTGAGTGATATCGGT
AATAAAACTGCGACGTTGAAAACCAGTAGCACCACGCAAGGTAATGTGGTGACGCAGCTTTCCAATCAGCAGCAGTCGAT
TTCCGGTGTCAATCTCGATGAGGAGTACGGAAATCTGCAACGTTTTCAGCAATATTATCTGGCGAATGCGCAGGTTCTGC
AGACGGCAAACGCGATTTTTGATGCGCTGATTAACATTCGCTAA

Upstream 100 bases:

>100_bases
AAACCTACAGCATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTG
AAGGCTTATAAGGAACCTCC

Downstream 100 bases:

>100_bases
GGGGAGATAAAATGCGTTTCAGTACCCAGATGATGTACCAGCAAAACATGCGCGGCATCACCAATTCTCAGGCAGAATGG
ATGAAGTACGGTGAACAGAT

Product: flagellar hook-associated protein FlgK

Products: NA

Alternate protein names: HAP1 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQT
QSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGST
ARQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDAN
GDAGEDFFAIGKPAVLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELT
FTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIG
NKTATLKTSSTTQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR

Sequences:

>Translated_547_residues
MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQT
QSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGST
ARQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDAN
GDAGEDFFAIGKPAVLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELT
FTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIG
NKTATLKTSSTTQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR
>Mature_546_residues
SSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQ
SSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIA
IGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGSTA
RQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANG
DAGEDFFAIGKPAVLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTF
TGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIGN
KTATLKTSSTTQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR

Specific function: Unknown

COG id: COG1256

COG function: function code N; Flagellar hook-associated protein

Gene ontology:

Cell location: Secreted. Bacterial flagellum (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flagella basal body rod proteins family [H]

Homologues:

Organism=Escherichia coli, GI1787323, Length=547, Percent_Identity=99.4515539305302, Blast_Score=1086, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010930
- InterPro:   IPR001444
- InterPro:   IPR002371 [H]

Pfam domain/function: PF06429 DUF1078; PF00460 Flg_bb_rod [H]

EC number: NA

Molecular weight: Translated: 57916; Mature: 57785

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYV
CHHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCCCCEEE
SGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTL
ECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLN
HCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHH
DQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGST
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEEECCEEEECCCC
ARQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQ
HHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCEEEECCCCHHHHHHHHHH
LALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPAVLQNTKNKGDVAIGATVTDASAVLAT
HHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCCCEEEEEEECCCCEEEEE
DYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTPAVNDSFTLKPVSDAI
EEEEEECCCEEEEEEEECCCEEEECCCCCCEEEECCEEEEEECCCCCCCCEEECCHHHHH
VNMDVLITDEAKIAMASAEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIG
EEEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
NKTATLKTSSTTQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIF
CCEEEEEECCCCCCCEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DALINIR
HHHHCCC
>Mature Secondary Structure 
SSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQANSTLGAGGWVGNGVYV
HHHHHHHHHCCHHHHHHHHHHCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCCCCEEE
SGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTSTSSLATQMQDFFTSLQTL
ECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNIAIGASVDQINNYAKQIASLN
HCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHH
DQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMANGYSLVQGST
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEEECCEEEECCCC
ARQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLNTGSLGGILTFRSQDLDQTRNTLGQ
HHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCEEEECCCCHHHHHHHHHH
LALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPAVLQNTKNKGDVAIGATVTDASAVLAT
HHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCCCEEEEEEECCCCEEEEE
DYKISFDNNQWQVTRLASNTTFTVTPDANGKVAFDGLELTFTGTPAVNDSFTLKPVSDAI
EEEEEECCCEEEEEEEECCCEEEECCCCCCEEEECCEEEEEECCCCCCCCEEECCHHHHH
VNMDVLITDEAKIAMASAEDAGDSDNRNGQALLDLQSNSKTVGGAKSFNDAYASLVSDIG
EEEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHC
NKTATLKTSSTTQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIF
CCEEEEEECCCCCCCEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
DALINIR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 8158647 [H]