Click here to switch to the map view.

The map label for this gene is thiK

Identifier: 218700392

GI number: 218700392

Start: 2102612

End: 2103436

Strand: Reverse

Name: thiK

Synonym: ECIAI39_2054

Alternate gene names: 218700392

Gene position: 2103436-2102612 (Counterclockwise)

Preceding gene: 218700393

Following gene: 218700391

Centisome position: 40.99

GC content: 53.09

Gene sequence:

>825_bases
GTGCCGTTTCGCAGCAATAATCCCCTCACGCGCGACGAATTGCTGTCGTGCTTTTTCCCGCAATTTCATCCCGTCACGAC
GTTTAATAGTGGGCTTAGTGGCGGGAGTTTTCTCATTGAACATCAGGGCCAGCGTTTTGTTGTGCGTCAGCCGCACGATC
CTGATGCGCCGCGGTTCGCGTTCTTGCGCCAGTATCGGGCTTTATCACAACTACCCGCATGCATTGCACCGAAGCCGCAT
TTATATCTCCGTGACTGGATGGTGGTCGACTATCTGCCCGGCGAGGTAAAAACGTATTTGCCGGATACCAACGAACTGGC
AGGCTTGCTGTATTATCTACATCAACAACCGCGTTTTGGCTGGCGAATAACGCTGTTGCCGTTACTGGAACTGTACTGGC
AGCAAAGCGATCCGGTGAGGCGGACAGTGGGTTGGCTGCGAATGTTAAAACGTCTGCGCAAAGCGCGGGAACCACGGCCT
TTACGCTTAAGTCCATTGCATATGGATGTCCACGCCGGAAATTTAGTGCATAGCGCGTCAGGGTTAAAACTCATCGACTG
GGAGTATGCCGGAGATGGTGATATCGCGCTGGAACTGGCGGCGGTGTGGGTGGAAAATACTGACCAGCATCGGCAATTGG
TCAATGACTATGCCACTCGCGCGAAGATTTATCCGGCGCAATTATGGCGTCAGGTCAGGCGATGGTTTCCCTGGCTGCTG
ATGCTCAAAGCAGGGTGGTTTGAGTACCGCTGGCGACAAACCGGCGATCAACAATTTATCAGGCTGGCCGATGACACCTG
GCGGCAGCTATTAATAAAACAATAA

Upstream 100 bases:

>100_bases
TACGTGCTGTACTCCAGCGCCTCTGGCAACGTTAACGCTCCGACCCTACAAATGCAGCTGATGCTGGTGCAGACGGGCGA
AATTATCTGGTCAGGTAAAG

Downstream 100 bases:

>100_bases
GGAGAGCAGTGTGGGTCCAGTAATGTTGGATGTCGAAGGTTACGAACTGGACGCGGAAGAGCGTGAAATACTGGCGCATC
CGCTGGTGGGAGGGCTGATT

Product: thiamine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPFRSNNPLTRDELLSCFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPH
LYLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPVRRTVGWLRMLKRLRKAREPRP
LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ

Sequences:

>Translated_274_residues
MPFRSNNPLTRDELLSCFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPH
LYLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPVRRTVGWLRMLKRLRKAREPRP
LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
>Mature_273_residues
PFRSNNPLTRDELLSCFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHL
YLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPVRRTVGWLRMLKRLRKAREPRPL
RLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLLM
LKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate [H]

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787349, Length=274, Percent_Identity=97.0802919708029, Blast_Score=545, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.89 [H]

Molecular weight: Translated: 32489; Mature: 32358

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFRSNNPLTRDELLSCFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPRFA
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHH
FLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFG
HHHHHHHHHHCCHHCCCCCCEEEECEEEEEECCCCCEECCCCCHHHHHHHHHHHCCCCCC
WRITLLPLLELYWQQSDPVRRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSAS
EEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEEECCC
GLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
CCEEEEEEECCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
HHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PFRSNNPLTRDELLSCFFPQFHPVTTFNSGLSGGSFLIEHQGQRFVVRQPHDPDAPRFA
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHH
FLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPDTNELAGLLYYLHQQPRFG
HHHHHHHHHHCCHHCCCCCCEEEECEEEEEECCCCCEECCCCCHHHHHHHHHHHCCCCCC
WRITLLPLLELYWQQSDPVRRTVGWLRMLKRLRKAREPRPLRLSPLHMDVHAGNLVHSAS
EEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCEEECCC
GLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYATRAKIYPAQLWRQVRRWFPWLL
CCEEEEEEECCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ
HHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA