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The map label for this gene is 218700352

Identifier: 218700352

GI number: 218700352

Start: 2063279

End: 2063719

Strand: Reverse

Name: 218700352

Synonym: ECIAI39_2012

Alternate gene names: NA

Gene position: 2063719-2063279 (Counterclockwise)

Preceding gene: 218700353

Following gene: 218700351

Centisome position: 40.21

GC content: 55.1

Gene sequence:

>441_bases
ATGCCGTGGCAACCATTAAGACGATGCACAGAACCAGGCTGTAACAGGCGCGTGAAGTCCGGCAAGTGTGAGGAGCACAG
GCGGGCGGCATGGCGTGCAGAGGATGCCAGACGAGGACACCGCCGCGCGCGTGGGTACTCCAGACAGTGGGACAAATACC
GCGCCATGTATCTGAGTAAAAACCCGTTATGCGTGCGATGCCTTGAGAAGGGGATATATACGCCCGCCGTGGTGGTGGAT
CACATTATCCCCATCGATGGCGGTGATGATGTTCTCTTCTGGCCCGAATGGAACCATCAACCATTGTGTCAGGCGTGCCA
TAACCAGAAAACGAAATGGCTTGATCCGGCAACAAAAAGCAAGCGTGCCGCAGGTGGATTTCGTGAAGAGGAAGAACGGG
CCGCTAACCGCAATAACTGGATGTATGACGCTGATGAATGA

Upstream 100 bases:

>100_bases
TGTACGAATACAGGGCCACGGTCGGGGATGGAGACACAAAAGAAATCCCGCTGGCTGTAGGGATGCTCTGGTCGGTTTAT
CGTGATGTGGGGGTGTACTG

Downstream 100 bases:

>100_bases
GCGGGAACAAAACCGCCTTATCCGTGGACTTATAAGGCAGCGTGACGCATGGAAGACACAGGAAACAGGGCATAAAGATA
AAGCGTCAGGACGCGCAGAA

Product: putative endonuclease

Products: NA

Alternate protein names: HNH Endonuclease Family Protein; Restriction Endonuclease; Phage Associated Protein; Holin Protein; Prophage; Phage Endonuclease; Phage Holin

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRARGYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVD
HIIPIDGGDDVLFWPEWNHQPLCQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE

Sequences:

>Translated_146_residues
MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRARGYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVD
HIIPIDGGDDVLFWPEWNHQPLCQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE
>Mature_145_residues
PWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRARGYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDH
IIPIDGGDDVLFWPEWNHQPLCQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE

Specific function: Unknown

COG id: COG1403

COG function: function code V; Restriction endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 17225; Mature: 17093

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRARGYSRQWDKYRAMYLSK
CCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCC
NPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPLCQACHNQKTKWLDPATKS
CCHHHHHHHCCCCCHHHHHHHEECCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCHH
KRAAGGFREEEERAANRNNWMYDADE
HHHCCCCCHHHHHHHCCCCCCEECCC
>Mature Secondary Structure 
PWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRARGYSRQWDKYRAMYLSK
CCHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCC
NPLCVRCLEKGIYTPAVVVDHIIPIDGGDDVLFWPEWNHQPLCQACHNQKTKWLDPATKS
CCHHHHHHHCCCCCHHHHHHHEECCCCCCCEEECCCCCCCHHHHHHHCCCCCCCCCCCHH
KRAAGGFREEEERAANRNNWMYDADE
HHHCCCCCHHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA