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The map label for this gene is gam

Identifier: 218700280

GI number: 218700280

Start: 2005184

End: 2005714

Strand: Reverse

Name: gam

Synonym: ECIAI39_1935

Alternate gene names: NA

Gene position: 2005714-2005184 (Counterclockwise)

Preceding gene: 218700281

Following gene: 218700279

Centisome position: 39.08

GC content: 49.34

Gene sequence:

>531_bases
ATGGCAAAAAGTACAAAAGGTGCAAAACGTATCAAGGCCGCAGCAGCACTCTGGGTGCCGGGGACACGTGAAGAGGTCAT
TGAGGGAATCAGACTACTCGGTGACGCACAACGTGAACTGGTCAGGGCTGAAACAGAAATGAATGACGCCATTGGCGATA
TCACCGCACGTTATGCCCCGCTCACCGAGAGCCTGAAAAAACGCATGGCCGAACTGCAGTCCGGTATTCAGACATGGTGT
GAGGCACACCGTGATGAACTGACCGGCAACGGGAAGGTGAAGTTCGCTAACCTCACCACCGGCGAGGTGCAGTGGCGAAA
CCGTCCGCCATCAGTCAGTATCCGTGGGGCGGATAATGTTATTGAATTACTGAGACGTCTGGGGCTTGAGCGCTTTATTC
GTGTAAAAGAGGAAATAAATAAAGACGCTATCCTGAATGAAAAAGAGGCCGTGAAAAATATTCCCGGTATTTCCATTAAA
AGCGACATTGAGGATTTTTCAATAATTCCTTTTGAGCAGGATGTGCAGTAA

Upstream 100 bases:

>100_bases
CAGGGGATATTTCCGTCAGGGCGTTTTCGAACTGCACGGCTGTCGCGTCGTGTGGCCGGAATCTTTGTGGTAATCAGCAC
AACTGGAGAAATCATAAAAA

Downstream 100 bases:

>100_bases
ACACACCACGTTAATTATTTAATAAAAACATTTTCTTTTTTATTCCGGCGTCAGCGCCGCGGGCTTCTGCACGCCGGAAA
CAGAGGAGAATTAAATCATG

Product: Host-nuclease inhibitor protein from bacteriophage

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAETEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWC
EAHRDELTGNGKVKFANLTTGEVQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIK
SDIEDFSIIPFEQDVQ

Sequences:

>Translated_176_residues
MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAETEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWC
EAHRDELTGNGKVKFANLTTGEVQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIK
SDIEDFSIIPFEQDVQ
>Mature_175_residues
AKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAETEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWCE
AHRDELTGNGKVKFANLTTGEVQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIKS
DIEDFSIIPFEQDVQ

Specific function: Protects linear double stranded DNA of Mu genome from exonuclease degradation [H]

COG id: COG4396

COG function: function code R; Mu-like prophage host-nuclease inhibitor protein Gam

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To phage Mu protein gam [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009951 [H]

Pfam domain/function: PF07352 Phage_Mu_Gam [H]

EC number: NA

Molecular weight: Translated: 19724; Mature: 19593

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAETEMNDAIGDITARYAP
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTESLKKRMAELQSGIQTWCEAHRDELTGNGKVKFANLTTGEVQWRNRPPSVSIRGADNV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCEEECCHHHH
IELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIKSDIEDFSIIPFEQDVQ
HHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHCCCCCCCCCCCCCCEECCCCCCCC
>Mature Secondary Structure 
AKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAETEMNDAIGDITARYAP
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTESLKKRMAELQSGIQTWCEAHRDELTGNGKVKFANLTTGEVQWRNRPPSVSIRGADNV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCEEECCHHHH
IELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIKSDIEDFSIIPFEQDVQ
HHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHCCCCCCCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]