Click here to switch to the map view.

The map label for this gene is dadA

Identifier: 218700230

GI number: 218700230

Start: 1961935

End: 1963233

Strand: Reverse

Name: dadA

Synonym: ECIAI39_1881

Alternate gene names: 218700230

Gene position: 1963233-1961935 (Counterclockwise)

Preceding gene: 218700232

Following gene: 218700229

Centisome position: 38.25

GC content: 56.51

Gene sequence:

>1299_bases
ATGCGAGTTGTCATACTGGGAAGTGGTGTGGTAGGCGTTGCCAGCGCCTGGTACTTAAATCAGGCAGGACATGAGGTCAC
CGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCAGCAC
CGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGAC
GGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCG
GATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTC
AGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCC
GGCGTACCGTACCAGCTGCTGGAATCCAGCCGTCTGGCGGAAGTGGAGCCCGCGCTGGCAGAAGTGGCGCACAAACTGAC
GGGGGGGCTCCAGCTGCCCAATGATGAAACCGGAGACTGCCAGCTGTTTACCCAGAATCTGGCGCGGATGGCGGAGCAGG
CGGGGGTTAAATTCCGCTTTAATACGCCCGTTGACCAACTGCTTTGCGACGGCGAGCAAATCTACGGCGTGAAGTGTGGC
GATGAAGTGATTAAGGCCGATGCATATGTGATGGCATTTGGTTCTTACTCGACGGCGATGCTCAAAGGCATTGTTGATAT
TCCGGTGTATCCGCTGAAAGGCTACTCGCTGACTATTCCCATTGCGCAGGAAGATGGTGCGCCGGTATCCACCATTCTTG
ATGAAACCTACAAAATCGCCATTACCCGTTTCGATAACCGCATTCGTGTTGGTGGAATGGCGGAAATTGTTGGCTTTAAT
ACCGAGCTGTTGCAACCGCGTCGTGAAACGCTGGAGATGGTGGTTCGCGATCTCTATCCACGCGGCGGTCATGTCGAGCA
GGCGACTTTCTGGACGGGTCTGCGCCCGATGACGCCAGACGGCACGCCGGTTGTCGGGCGTACACGCTTTAAAAATCTGT
GGCTGAATACCGGTCACGGCACGCTCGGCTGGACGATGGCTTGCGGTTCCGGTCAGTTGTTAAGCGATCTGCTCTCTGGT
CGCACGCCAGCGATCCCGTATGAAGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGCCCGGGCCATTT
ACATGGCGCACACAGCTAA

Upstream 100 bases:

>100_bases
ACCATAAAAAAGCCGTATGTTGAATAATATTTTCAACTGAGTTATCAAGATGTAATTAGATTATTATTCTTTTACTGTAT
CTACCGTTATCGGAGTGGCT

Downstream 100 bases:

>100_bases
GGAAACGAGATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGGCAGGC
CGCGCCGCACGCGCGCGTTT

Product: D-amino acid dehydrogenase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLD
GTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCG
DEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFN
TELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSG
RTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS

Sequences:

>Translated_432_residues
MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLD
GTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCG
DEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFN
TELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSG
RTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS
>Mature_432_residues
MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLD
GTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCG
DEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFN
TELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSG
RTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=432, Percent_Identity=100, Blast_Score=896, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 47608; Mature: 47608

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGV
CEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCC
PLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLK
CHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHH
ALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPALA
HHHHHCCEEECCCCCCEEEEEECHHHHHCHHCCEEEEECCCCCHHHHCCCCCHHCCHHHH
EVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCEEEEEECC
DEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIA
HHHHHCCEEEEEECCHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCCHHHHHCCCEEEE
ITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPD
EEEECCEEEECCEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCC
GTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYSRGFT
CCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
PSRPGHLHGAHS
CCCCCCCCCCCC
>Mature Secondary Structure
MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGV
CEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCC
PLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLK
CHHHHHHHHHCCCCEEEEECCCEEHHHHHHHHHHCCCHHHHHHCCCCEEEEHHHHHHHHH
ALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPALA
HHHHHCCEEECCCCCCEEEEEECHHHHHCHHCCEEEEECCCCCHHHHCCCCCHHCCHHHH
EVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCEEEEEECC
DEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIA
HHHHHCCEEEEEECCHHHHHHHHHHHCCCEEECCCEEEEEEECCCCCCHHHHHCCCEEEE
ITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPD
EEEECCEEEECCEEEEECCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHEECCCCCCCCC
GTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYSRGFT
CCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCHHHHHHCCCC
PSRPGHLHGAHS
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA