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The map label for this gene is dadX

Identifier: 218700229

GI number: 218700229

Start: 1960855

End: 1961925

Strand: Reverse

Name: dadX

Synonym: ECIAI39_1880

Alternate gene names: 218700229

Gene position: 1961925-1960855 (Counterclockwise)

Preceding gene: 218700230

Following gene: 218700224

Centisome position: 38.23

GC content: 57.7

Gene sequence:

>1071_bases
ATGACCCGTCCGATACAGGCCAGCCTCGATCTGCAGGCATTAAAACAGAATCTGTCCATTGTCCGGCAGGCCGCGCCGCA
CGCGCGCGTTTGGTCGGTGGTAAAAGCGAATGCTTACGGGCACGGTATTGAGCGTATCTGGAGCGCGCTCGGGGCCACCG
ATGGCTTTGCATTACTTAATCTGGAAGAGGCAATAACGTTACGTGAGCGCGGCTGGAAAGGACCGATCCTGATGCTGGAG
GGATTTTTCCATGCTCAGGATCTGGAGATTTATGACCAGCACCGCCTGACCACCTGCGTACACAGCAACTGGCAACTCAA
AGCACTGCAAAATGCACGGCTAAAAGCGCCGTTGGATATTTATCTTAAAGTGAACAGTGGGATGAATCGGTTGGGCTTCC
AGCCCGATCGCGTGCTTACCGTCTGGCAGCAGTTGCGGGCGATGGCGAATGTTGGCGAAATGACCCTGATGTCGCATTTT
GCCGAGGCGGAACATCCTGATGGAATTTCCAGCGCGATGGCGCGTATTGAGCAGGCGGCGGAAGGGCTGGAGTGTCGGCG
TTCGTTGTCCAATTCGGCGGCGACTCTGTGGCACCCGGAAGCGCATTTTGACTGGGTTCGGCCTGGCATTATTTTGTATG
GCGCTTCGCCGTCCGGTCAGTGGCGTGATATCGCCAATACCGGATTACGTCCGGTGATGACGCTAAGCAGTGAGATTATT
GGTGTCCAGACGCTAAAAGCGGGCGAGCGTGTGGGCTACGGCGGTCGCTATACTGCGCGCGATGAGCAGCGAATCGGCAT
TGTCGCCGCAGGATACGCCGACGGTTATCCGCGCCATGCGCCTACCGGTACTCCTGTTTTAGTGGACGGCGTGCGCACCA
TGACGGTGGGGACCGTCTCGATGGATATGCTGGCGGTTGATTTAACGCCTTGCCCGCAGGCGGGGATTGGTACGCCGGTT
GAGCTGTGGGGCAAGGAGATCAAAATTGATGATGTCGCCGCCGCTGCCGGAACGGTGGGCTATGAGTTGATGTGCGCGCT
GGCGTTACGCGTCCCGGTTGTGACGGTGTAA

Upstream 100 bases:

>100_bases
AGCGATCCCGTATGAAGATCTAAGCGTAGCGCGCTACAGCCGTGGATTTACGCCATCACGCCCGGGCCATTTACATGGCG
CACACAGCTAAGGAAACGAG

Downstream 100 bases:

>100_bases
CTTGTTGTAAGCCGGATCGGAGGCAACGTCTTCTGGTTGCAAAAAAAATCATCCATCCGGCTGGTCAGCAACTGTAGTTG
TTAATGTGACAGAGCCATTG

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Sequences:

>Translated_356_residues
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLE
GFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPV
ELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
>Mature_355_residues
TRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLNLEEAITLRERGWKGPILMLEG
FFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFA
EAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEIIG
VQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVE
LWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1787439, Length=356, Percent_Identity=99.1573033707865, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1790487, Length=346, Percent_Identity=42.7745664739884, Blast_Score=282, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 38871; Mature: 38740

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLN
CCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISSAMARIEQAA
EEEECCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
HHHHHHHHHCCCCEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure 
TRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIERIWSALGATDGFALLN
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEE
LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI
HHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEE
YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISSAMARIEQAA
EEEECCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII
HHHHHHHHHCCCCEEEECCCCCCCEECCCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS
EEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCEEEEEEEEE
MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV
EEEEEEECCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]