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The map label for this gene is yneF

Identifier: 218700143

GI number: 218700143

Start: 1877216

End: 1878634

Strand: Reverse

Name: yneF

Synonym: ECIAI39_1786

Alternate gene names: 218700143

Gene position: 1878634-1877216 (Counterclockwise)

Preceding gene: 218700144

Following gene: 218700142

Centisome position: 36.61

GC content: 48.91

Gene sequence:

>1419_bases
ATGCATGTACAGCCCATTTCAACTTTCCGTTTGTTCCAGGAAGGTCATCTGCTACGTAATAGCATCGCTATTTTTGCGCT
AACCACGCTGTTCTATTTCATTGGTGCAGAGTTACGGCTGGTTCACGAACTTTCTCTTTTCTGGCCGCTGAATGGCGTAA
TGGCGGGGGTGTTTGCCCGCTATGTCTGGCTTAATCGACTGCATTACTATGCGATCAGTTATGTGGCGATGCTGGTTTAT
GATGCCATAACCACCGAATGGGGGCTGGTTTCACTGGCTATCAATTTCTCCAATATGATGTTTATTGTTACCGTCGCCTT
ACTGGTCGCGCGGGATAAGCGTCTGGGGAAGAATAAGTATGAGCCAGTAAGTGCCTTACGGCTATTTAATTACTGTCTGA
TTGCCGCATTATTATGCGCTATTGTCGGGGCGATTGGTTCGGTCAGTATTGATAGTCTGGATTTCTGGCCTTTGCTTGCC
GACTGGTTCAGTGAGCAATTCTCAACGGGCGTGTTGATCGTGCCTTGTATGCTGACGTTGGCAATTCCTGGAGTGCTGCC
GCGCTTTAAAGCAGAACAGATGATGCCCGCTATCGCGCTTATTGTGTCGGTTATTGCCTCGGTAGTCATTGGCGGAGCGG
GGAGTCTGGCGTTCCCGCTGCCTGCATTAATCTGGTGTGCAGTGCGCTATACGCCGCAGGTAACATGTCTGTTGACCTTT
GTCACCGGTGCGGTGGAAATCGTAATGGTGGCAAATTCGGTGATTGATATCTCGGTCGGTTCGCCGTTCTCCATTCCGCA
AATGTTCTCCGCACGTCTCGGTATTGCCACGATGGCGATATGCCCAATTATGGTTTCTTTTAGCGTGGCAGCGATCAATT
CGCTAATGAAGCAAGTTGCGCTGCGAGCCGACTTTGATTTTCTGACTCAGGTTTACTCACGGTCCGGTCTTTATGAGGCG
CTGAAAAGTCCATCGCTGAAACAGACACAACATCTGACTGTCATGCTGCTTGATATCGACTATTTCAAAAGCATTAACGA
TAACTATGGACATGAATGTGGCGATAAAGTGTTAAGCGTGTTTGCTCAGCATATTCAGAAGATTGTCGGTGATAAGGGGC
TGGTGGCGCGAATGGGCGGCGAGGAATTTGCTGTTGCAGTGCCATCGGTGAATCCTGTCGATGGTCTGCTAATGGCGGAA
AAAATCCGTAAAGGCGTTGAACTGCAACCGTTCACCTGGCAACAAAAAACGCTTTATCTCACGGTAAGTATTGGCGTCGG
TAGTGGTTGCGCATCGTACCGAACTCTGACCGATGACTTTAATAAATTGATGGTCGAAGCCGATACATGTCTGTATCGCT
CGAAGAAAGATGGGCGCAACCGTACCAGCACTATGCGTTACGGTGAGGAAGTTGTCTGA

Upstream 100 bases:

>100_bases
CTTTATTTGCCGCCGCTGTCAGCGAACATGCTATCCTTTATAAGAATTTACTTATTACGTTGAGATAATTGCCACCCGCA
ATTGTATGGACAGATAATTA

Downstream 100 bases:

>100_bases
CATCTTTATTCATTCACAGCGATTTAAATGGAATGTGTTCTACCACTTTATTGAGCGAATAAAAGGCGTAGTTCCTCGAT
TAAACCATGATCCGCGCCAC

Product: putative membrane-associated diguanylate cyclase (GGDEF domain)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MHVQPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVY
DAITTEWGLVSLAINFSNMMFIVTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTF
VTGAVEIVMVANSVIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEA
LKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAE
KIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV

Sequences:

>Translated_472_residues
MHVQPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVY
DAITTEWGLVSLAINFSNMMFIVTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTF
VTGAVEIVMVANSVIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEA
LKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAE
KIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV
>Mature_472_residues
MHVQPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHELSLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVY
DAITTEWGLVSLAINFSNMMFIVTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTF
VTGAVEIVMVANSVIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEA
LKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAE
KIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=315, Percent_Identity=98.4126984126984, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=37.4269005847953, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787262, Length=212, Percent_Identity=30.188679245283, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=184, Percent_Identity=33.695652173913, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788381, Length=193, Percent_Identity=28.4974093264249, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=201, Percent_Identity=27.8606965174129, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=30.939226519337, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787816, Length=180, Percent_Identity=30.5555555555556, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693134, Length=133, Percent_Identity=33.0827067669173, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=178, Percent_Identity=33.1460674157303, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 52081; Mature: 52081

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MHVQPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHELSLFWPLNGVMAGVFAR
CCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHH
YVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFIVTVALLVARDKRLGKNKY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCC
EPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLADWFSEQFSTGVLIVPCMLTL
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHHH
AIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAFPLPALIWCAVRYTPQVTCLLTF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
VTGAVEIVMVANSVIDISVGSPFSIPQMFSARLGIATMAICPIMVSFSVAAINSLMKQVA
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTVMLLDIDYFKSINDNYGHECGDKVLSV
HHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHH
FAQHIQKIVGDKGLVARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYL
HHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEECCEEEEE
TVSIGVGSGCASYRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]