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The map label for this gene is gadC

Identifier: 218700121

GI number: 218700121

Start: 1846107

End: 1847642

Strand: Reverse

Name: gadC

Synonym: ECIAI39_1757

Alternate gene names: 218700121

Gene position: 1847642-1846107 (Counterclockwise)

Preceding gene: 218700122

Following gene: 218700120

Centisome position: 36.0

GC content: 48.44

Gene sequence:

>1536_bases
ATGGCTACATCAGTACAGACAGGTAAAGCTAAGCAGCTCACATTACTCGGATTCTTTGCCATAACGGCATCGATGGTAAT
GGCTGTTTATGAATACCCTACCTTCGCAACATCGGGCTTTTCATTAGTCTTCTTCCTGCTATTAGGCGGGATTTTATGGT
TTATTCCCGTGGGACTTTGTGCTGCGGAAATGGCTACCGTCGACGGCTGGGAAGAAGGTGGCGTCTTCGCCTGGGTATCA
AATACTCTGGGGCCGAGATGGGGATTTGCAGCGATCTCATTTGGCTATCTGCAAATCGCCATTGGTTTTATTCCGATGCT
CTATTTCGTGTTAGGGGCACTCTCCTACATCCTGAAATGGCCAGCGCTGAATGAAGACCCCATTACCAAAACTATTGCAG
CACTCATCATTCTTTGGGCGCTGGCATTAACGCAGTTTGGTGGCACGAAATACACGGCGCGAATTGCTAAAGTTGGCTTC
TTCGCCGGTATCCTGTTACCTGCATTTATTTTGATCGCATTAGCGGCTATTTATCTGCACTCCGGTGCCCCCGTTGCTAT
CGAAATGGATGCGAAGACCTTCTTCCCTGACTTCTCTAAAGTGGGCACTCTGGTTGTATTTGTTGCCTTCATTTTGAGTT
ATATGGGCGTTGAAGCTTCCGCAACCCACGTCAATGAAATGAGCAACCCCGGGCGCGACTATCCACTGGCTATGTTACTG
CTGATGGTGGCGGCAATCTGCTTAAGCTCTGTTGGCGGTTTGTCTATTGCGATGGTCATTCCGGGTAATGAAATCAACCT
CTCCGCAGGGGTAATGCAAACCTTTACCGTTCTGATGTCACATGTGGCACCGGAAATTGAGTGGACGGTTCGCGTGATCT
CCGCACTGCTGTTGCTGGGTGTTCTGGCGGAAATCGCTTCCTGGATTGTTGGTCCTTCTCGCGGGATGTATGTCACAGCG
CAGAAAAACCTGCTGCCAGCGGCATTCGCTAAAATGAACAAAAATGGCGTACCGGTAACGCTGGTCATTTCGCAGTTGGT
GATTACTTCTATCGCGTTGATCATCCTCACCAATACCGGTGGCGGTAACAACATGTCCTTCCTGATCGCACTGGCGCTGA
CGGTGGTGATTTATCTGTGTGCTTATTTCATGCTGTTTATTGGCTACATTGTGTTGGTTCTTAAACATCCTGACTTAAAA
CGCACATTTAATATCCCTGGTGGTAAAGGGGTGAAACTGGTCGTGGCTATTGTCGGTCTGCTGACTTCAATTATGGCGTT
TATTGTTTCCTTCCTGCCGCCGGATAACATCCAGGGTGACTCTACTGATATGTATGTTGAATTACTGGTCGTGAGTTTCC
TGGTGGTACTTGCCCTGCCCTTTATTCTCTACGCTGTTCATGATCGTAAAGGCAAAGGGAATACCGGCGTCACTCTGGAG
CCAATCAACAGTCAGAACGCACCAAAAGGTCACATCTTCCTGCACCCGCGTGCACGTTCACCACACTATATTGTGATGAA
TGACAAGAAACACTAA

Upstream 100 bases:

>100_bases
AGTCTGTTCACTGGCATTAGCAACGGAAAATATTGTTCTGAATACGCTTCAGAACAAAACAGGTGCGGTTCCGACAGGAA
TACCGTTTTAGGGGGATAAT

Downstream 100 bases:

>100_bases
CCATGTCTGAATGGTTATAAGATAAGGGAGCGATAATTCATCGCTCCCTTTTTCGTGCTTGCTGTCGTATTGACTTAACC
GGGATAAGTACGAGAATGAG

Product: glutamate:gamma-aminobutyric acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: Extreme acid sensitivity protein [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVS
NTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTA
QKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLK
RTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKGNTGVTLE
PINSQNAPKGHIFLHPRARSPHYIVMNDKKH

Sequences:

>Translated_511_residues
MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVS
NTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTA
QKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLK
RTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKGNTGVTLE
PINSQNAPKGHIFLHPRARSPHYIVMNDKKH
>Mature_510_residues
ATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSN
TLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGFF
AGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLLL
MVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQ
KNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKR
TFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKGNTGVTLEP
INSQNAPKGHIFLHPRARSPHYIVMNDKKH

Specific function: Involved in glutamate-dependent acid resistance. Imports glutamate inside the cell while simultaneously exporting to the periplasm the GABA produced by gadA and gadB. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Glutamate:GABA antiporter (GGA) (TC 2.A.3.7) family [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=511, Percent_Identity=99.412915851272, Blast_Score=1016, Evalue=0.0,
Organism=Escherichia coli, GI87082380, Length=436, Percent_Identity=24.3119266055046, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081798, Length=402, Percent_Identity=24.1293532338308, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004759 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 55013; Mature: 54882

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLC
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKW
HHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGFFAGILLPAFILIALAAIYLH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
CCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHH
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLG
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTG
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCC
GGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKRTFNIPGGKGVKLVVAIVGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHH
LTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKGNTGVTLE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
PINSQNAPKGHIFLHPRARSPHYIVMNDKKH
ECCCCCCCCCEEEEECCCCCCCEEEECCCCC
>Mature Secondary Structure 
ATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKW
HHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGFFAGILLPAFILIALAAIYLH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
CCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHH
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLG
HHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTG
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEECCC
GGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKRTFNIPGGKGVKLVVAIVGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHH
LTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKGNTGVTLE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
PINSQNAPKGHIFLHPRARSPHYIVMNDKKH
ECCCCCCCCCEEEEECCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]