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The map label for this gene is ycjW

Identifier: 218700043

GI number: 218700043

Start: 1744520

End: 1745518

Strand: Reverse

Name: ycjW

Synonym: ECIAI39_1672

Alternate gene names: 218700043

Gene position: 1745518-1744520 (Counterclockwise)

Preceding gene: 218700047

Following gene: 218700032

Centisome position: 34.01

GC content: 45.75

Gene sequence:

>999_bases
ATGTCCCCTACTATTTATGATATTGCCAGGGTTGCAGGCGTATCAAAATCCACCGTATCACGCGTGCTGAATAAGCAAAC
CAATATCTCCCCGGAAGCGCGCGAAAAAGTGTTACGGGCCATTGAAGAATTACAGTATCAACCAAACAAGCTGGCACGCG
CGCTGACCTCTTCGGGTTTTGATGCCATTATGGTGATTTCTACCCGTTCGACCAAAACTACGGCGGGTAATCCGTTTTTC
TCGGAAGTTTTACATGCCATCACCGCCAAAGCTGAAGAAGAAGGTTTCGACGTGATATTGCAGACGTCGCACAACCTGGC
AGAAGACTTACAAAAATGCGAAAGCAAAATTAAGCAGAAAATGATTAAAGGCATTATTATGCTAAGTTCGCCAGCGGATG
AGTCATTTTTTGCCCAACTCGATAAATATGATATTCCGGTGGTGGTAATTGGCAAAGTTGAAGGTCAATATGCCCATGTT
TATTCTGTCGATACCGATAATTTTGGCGACAGCATTGCGTTGACCGATGCGTTAATTGAAAGTGGGCATCAAAATATTGC
CTGCCTGCATGCTCCGCTTGATGTCCATGTTTCGGTTGACCGCGTTAATGGCTATAAGCAAAGTCTGGCTGCGCATAATA
TTGCAGTGCGTGATGAATGGATTGTTGACGGCGGTTATACCCATGAAACAGCCTTGCAAGCCGCACGGCAATTATTAAGC
CAGTCGCCGTTGCCAGAAGCCGTATTTGCCACTGACAGCCTGAAATTAATGAGCATTTATCGTGCGGCAGCAGAGAAAAA
TATTGCTATTCCGCAGCAGTTAGCGGTGGTGGGTTATAGCAATGAAACGCTGTCATTTATTTTAACGCCTGCACCGAGCG
GCATCGATGTTCCGACGCAGGAGTTAGGGCAACAAAGCTGCGAGTTATTATTCCGCTTAATTGCCGGAAAACCGTCACCA
CAAAATATTACCGTTGCCACGCATATGTCGTTGAAATAA

Upstream 100 bases:

>100_bases
TGTGGGGGCCAGGCCATTGATTATCAACTACCCGTGTTGTCATCGACGGGCGAACTCCATAAGATGTGCAGCATTCACGT
TCGGACGGAGTTCCTGACCT

Downstream 100 bases:

>100_bases
ATGTATGCCGGATTTGGCGCTCGCGCCGCATCCGGCAATTTAACCCATTATCAGAACGAGTAATTTACGCCGACACCTGC
ATAATGGAATTTATCGCTGT

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPSGIDVPTQELGQQSCELLFRLIAGKPSP
QNITVATHMSLK

Sequences:

>Translated_332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPSGIDVPTQELGQQSCELLFRLIAGKPSP
QNITVATHMSLK
>Mature_331_residues
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFS
EVLHAITAKAEEEGFDVILQTSHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVY
SVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLSQ
SPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPSGIDVPTQELGQQSCELLFRLIAGKPSPQ
NITVATHMSLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=98.4939759036145, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=324, Percent_Identity=30.8641975308642, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1790194, Length=312, Percent_Identity=30.4487179487179, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=29.4117647058824, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=27.2151898734177, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=29.0625, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789202, Length=320, Percent_Identity=26.875, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=25.2365930599369, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786540, Length=323, Percent_Identity=25.6965944272446, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787906, Length=313, Percent_Identity=26.1980830670927, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786268, Length=289, Percent_Identity=23.8754325259516, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790689, Length=335, Percent_Identity=27.4626865671642, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790715, Length=322, Percent_Identity=22.6708074534161, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36151; Mature: 36019

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNLAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPSGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLIAGKPSPQNITVATHMSLK
HHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNLAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHVYSVDTDNFGDSIALTDALIE
HHHCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
SGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPSGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFRLIAGKPSPQNITVATHMSLK
HHHHHHHHHHHHHHHCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]