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The map label for this gene is puuE

Identifier: 218700024

GI number: 218700024

Start: 1727154

End: 1728419

Strand: Direct

Name: puuE

Synonym: ECIAI39_1653

Alternate gene names: 218700024

Gene position: 1727154-1728419 (Clockwise)

Preceding gene: 218700023

Following gene: 218700026

Centisome position: 33.65

GC content: 57.27

Gene sequence:

>1266_bases
ATGAGCAACAATGAATTCCATCAGCGTCGTCTTTCTGCTACCCCACGCGGGGTTGGCGTGATGTGTAACTTCTTCGCCCA
GTCGGCAGAAAACGCCACGCTGAAGGATGTAGAGGGCAACGAATACATCGATTTCGCCGCAGGCATTGCGGTACTGAATA
CCGGGCATCGCCACCCTGATCTGGTCGCGGCGGTGGAGCAGCAATTGCAACAGTTTACCCACACGGCGTATCAGATTGTG
CCGTACGAAAGCTACGTCACCCTGGCGGAGAAAATCAACGCCCTTGCCCCGGTGAGCGGGCAGGCTAAAACTGCGTTCTT
CACCACCGGTGCGGAAGCGGTGGAGAACGCGGTGAAAATCGCCCGCGCCCATACCGGACGCCCTGGCGTGATTGCGTTTA
GCGGCGGCTTCCACGGTCGTACATATATGACCATGGCGCTGACCGGAAAGGTCGCGCCGTACAAAATCGGCTTCGGCCCG
TTCCCCGGTTCGGTATATCACGTACCTTATCCGTCAGATTTACATGACGTTTCAACGCAGGACTCCCTCGACGCCATTGA
ACGCTTGTTTAAATCGGACATTGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAAGGCGGTTTCA
ACGTTGCACCAAAAGAGCTGGTTGCCGCCATTCGCCGCCTGTGCGACGAGCACGGTATTGTGATGATTGCTGATGAAGTG
CAAAGCGGCTTTGCGCGTACCGGTAAACTGTTTGCCATGGATCACTACGCCGATAAGCCGGACTTAATGACGATGGCGAA
AAGCCTCGCGGGCGGCATGCCGCTTTCGGGCGTGGTCGGTAACGCGAATATTATGGACGCGCCCGCGCCGGGCGGGTTGG
GTGGTACTTACGCCGGGAACCCGCTGGCGGTGGCTGCCGCGCACGCTGTGCTCAACATTATCGACAAAGAATCACTCTGT
GAACGCGCGAATCAACTGGGCCAGCGCCTGACAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATCGCGGCGGT
ACGCGGTCTGGGTTCTATGATTGCGGCAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCACAGAAAA
TCCAGCAACGCGCGCTGGCGCAGGGGCTGCTTCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGTTTCCTGTATCCG
CTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAATTTTGCAGGATGCGCTGAAAGATTAA

Upstream 100 bases:

>100_bases
CGCGTTCCGTTTACCGCGATGGGCGCGGCTTATTACAGCCTGCGCGATCGTCTGGGCGTTTAATTTCCGATTAACAGTGA
AGAGTCAAAAGGTGTGAAAC

Downstream 100 bases:

>100_bases
TACGCAGAAGCAGGGTCAACGCAATAATGTCTGATACGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATG
CCGGATAAGGCGTTCACGCC

Product: 4-aminobutyrate transaminase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIV
PYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
FPGSVYHVPYPSDLHDVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLC
ERANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYP
LTIPDAQFDAAMKILQDALKD

Sequences:

>Translated_421_residues
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIV
PYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
FPGSVYHVPYPSDLHDVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLC
ERANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYP
LTIPDAQFDAAMKILQDALKD
>Mature_420_residues
SNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVP
YESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPF
PGSVYHVPYPSDLHDVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEVQ
SGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCE
RANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPL
TIPDAQFDAAMKILQDALKD

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=400, Percent_Identity=32.5, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI284507298, Length=322, Percent_Identity=32.6086956521739, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI13994255, Length=416, Percent_Identity=31.0096153846154, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI37574042, Length=450, Percent_Identity=28.2222222222222, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI188536080, Length=445, Percent_Identity=28.314606741573, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI38679950, Length=445, Percent_Identity=28.314606741573, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI38679946, Length=445, Percent_Identity=28.314606741573, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI226442709, Length=391, Percent_Identity=29.156010230179, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI24119277, Length=392, Percent_Identity=27.5510204081633, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI226442705, Length=450, Percent_Identity=27.7777777777778, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI1787560, Length=421, Percent_Identity=98.812351543943, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=421, Percent_Identity=54.1567695961995, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1789759, Length=403, Percent_Identity=33.7468982630273, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1788044, Length=390, Percent_Identity=34.8717948717949, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI145693181, Length=406, Percent_Identity=34.9753694581281, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1786349, Length=348, Percent_Identity=31.6091954022989, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786991, Length=411, Percent_Identity=28.4671532846715, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25144271, Length=407, Percent_Identity=32.9238329238329, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI32564660, Length=422, Percent_Identity=32.4644549763033, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71992977, Length=410, Percent_Identity=30.4878048780488, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25144274, Length=295, Percent_Identity=32.5423728813559, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17541228, Length=427, Percent_Identity=25.7611241217799, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323470, Length=406, Percent_Identity=32.512315270936, Blast_Score=189, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6324432, Length=406, Percent_Identity=31.7733990147783, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6324386, Length=316, Percent_Identity=24.0506329113924, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321456, Length=372, Percent_Identity=26.6129032258064, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21357415, Length=405, Percent_Identity=32.0987654320988, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI161085790, Length=418, Percent_Identity=29.6650717703349, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI28574759, Length=408, Percent_Identity=30.3921568627451, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI21356575, Length=429, Percent_Identity=28.2051282051282, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281366494, Length=364, Percent_Identity=26.9230769230769, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24667139, Length=364, Percent_Identity=26.9230769230769, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24667143, Length=364, Percent_Identity=26.9230769230769, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 44760; Mature: 44628

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPD
CCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCCEEHHHHHHHHCCCCCCCHH
LVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
ARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHDVSTQ
HHHCCCCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCEEECCCCCCCCCCCCC
DSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
HHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHH
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGN
HCCHHHCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCEECCCCCCCCCCCCCCC
PLAVAAAHAVLNIIDKESLCERANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
PQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDALK
CCCCCCHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
D
C
>Mature Secondary Structure 
SNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPD
CCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCCEEHHHHHHHHCCCCCCCHH
LVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHH
ARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHDVSTQ
HHHCCCCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCEEECCCCCCCCCCCCC
DSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEV
HHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEHHH
QSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGN
HCCHHHCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEECCCCEECCCCCCCCCCCCCCC
PLAVAAAHAVLNIIDKESLCERANQLGQRLTNTLIDAKESVPAIAAVRGLGSMIAAEFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
PQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDALK
CCCCCCHHHHHHHHHHHHHHCCHHEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]