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The map label for this gene is dhaH

Identifier: 218699905

GI number: 218699905

Start: 1602582

End: 1604000

Strand: Reverse

Name: dhaH

Synonym: ECIAI39_1534

Alternate gene names: 218699905

Gene position: 1604000-1602582 (Counterclockwise)

Preceding gene: 218699906

Following gene: 218699904

Centisome position: 31.25

GC content: 50.04

Gene sequence:

>1419_bases
ATGGTAAACCTGGTCATAGTTTCACATAGCAGCCGACTGGGAGAAGGTGTCGGTGAATTAGCCCGTCAGATGTTAATGAG
TGATAGTTGTAAAATCGCTATTGCCGCGGGAATTGACGATCCACAAAATCCCATTGGTACCGATGCCGTCAAAGTGATGG
AGGCCATCGAATCTGTTGCCGATGCCGACCATGTGCTGGTCATGATGGATATGGGAAGCGCATTATTGAGTGCTGAAACT
GCGCTGGAATTGCTGGCTCCCGAGATCGCCGCAAAAGTACGTTTGTGTGCTGCGCCGTTGGTCGAAGGTACACTGGCAGC
AACGGTCAGCGCGGCCTCGGGGGCGGATATCGACAAAGTCATCTTTGACGCCATGCATGCGCTGGAAGCCAAACGTGAAC
AACTGGGTTTACCGTCCTCCGACACTGAAATCTCTGACACATGTCCTCCGTACGATGAAGAAGCCCGTTCTCTGTCGGTG
GTCATAAAAAACCGTAACGGCCTGCATGTACGTCCGGCCTCCCGACTGGTTTATACCTTATCGACATTTAATGCCGATAT
GTTGCTGGAGAAAAACGGCAAATGCGTTACACCGGAGAGTATTAATCAGATTGCGTTACTACAAGTTCGCTATAACGATA
CGCTGCGCCTGATTGCGAAAGGACCAGAAGCTGAAGAGGCACTGATCGCTTTCCGTCAGCTGGCTGAAGATAACTTTGGT
GAAACGGAGGAAGTTGCTCCACCTACTCTGCGTCCCGTTCCGCCTGTTTCGGGTAAAGCCTTTTATTATCAACCTGTTTT
ATGTACGGTACAGGCAAAATCAATCCTGACCGTGGAAGAAGAACAAGAACGATTACGCCAGGCCATTGACTTCACGTTAT
TAGATCTGATGACGTTAACAGCGAAAGCAGAAACCAGCGGGCTTGACGATATTGCCGCAATCTTTTCTGGTCACCATACA
CTGTTAGATGATCCGGAACTGCAGGCGGCGGCAAGCGAACTCCTTCAGCATGAACATTGCACGGCGGAATATGCCTGGCA
GCACGTTCTTAAAGAACTTAGCCAGCAATACCAACAGCTGGATGATGAATATCTACAGGCTCGCTATATCGATGTGGACG
ATCTTCTGCATCGCACCCAGGTCCACCTGACCCAAACGAAAGAAGAACTCCCGCAGTTTAACTCGCCAACTATTCTACTG
GCGGAGAACATTTATCCCTCCACAGTACTGCAACTGGATCCAGCGGTTGTAAAAGGTATCTGCCTTAGCGCCGGAAGTCC
GCTATCCCACAGTGCCCTAATCGCCCGTGAACTGGGAATTGGCTGGATTTGCCAGCAGGGTGAGAAACTGTATGCGATAC
AACCAGAAGAAACGCTAACGCTGGACGTTAAAACGCAACGTTTCAACCGTCAGGGTTAA

Upstream 100 bases:

>100_bases
CTCGGTGAACGCAGTATTGGTCACCAGGATCCCGGCGCGACCTCGGTGATGTTTATGATGCAAATGTTGGCGTTAGCCGC
AAAAGAGTAAGGAATTGGTG

Downstream 100 bases:

>100_bases
ACCACCGTTCGGATGGCATCATTCTGATGTCATCCGGCAATTTACTCTGCACTGATGTTTTTTAACTACCTGTTATATTT
TTCACCAAATTATGATGATT

Product: dihydroxyacetone kinase subunit M

Products: NA

Alternate protein names: Phosphocarrier protein HPr; Protein H; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSILTVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHT
LLDDPELQAAASELLQHEHCTAEYAWQHVLKELSQQYQQLDDEYLQARYIDVDDLLHRTQVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG

Sequences:

>Translated_472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSILTVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHT
LLDDPELQAAASELLQHEHCTAEYAWQHVLKELSQQYQQLDDEYLQARYIDVDDLLHRTQVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG
>Mature_472_residues
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVADADHVLVMMDMGSALLSAET
ALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSILTVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHT
LLDDPELQAAASELLQHEHCTAEYAWQHVLKELSQQYQQLDDEYLQARYIDVDDLLHRTQVHLTQTKEELPQFNSPTILL
AENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG

Specific function: Phosphotransferase subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaM serves as the phosphoryl donor. It is phosphorylated by HPr, then it donates its phosphoryl group to dhaL-ADP, which eventually tran

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI226510935, Length=472, Percent_Identity=98.0932203389831, Blast_Score=946, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=208, Percent_Identity=30.2884615384615, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI48994992, Length=311, Percent_Identity=25.4019292604502, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012844
- InterPro:   IPR008279
- InterPro:   IPR004701
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731 [H]

Pfam domain/function: PF03610 EIIA-man; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 51600; Mature: 51600

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00369 PTS_HPR_HIS ; PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCHHHH
IFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSVVIKNRNGLHVRPASRLVYTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCEEECCHHHEEEEE
STFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ECCCCEEEEECCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCC
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSILTVEEEQERLRQAIDFTLLDLMTLT
CCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AKAETSGLDDIAAIFSGHHTLLDDPELQAAASELLQHEHCTAEYAWQHVLKELSQQYQQL
HHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDEYLQARYIDVDDLLHRTQVHLTQTKEELPQFNSPTILLAENIYPSTVLQLDPAVVKGI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHEEECHHHHHHH
CLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG
HHCCCCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEEEEHHHCCCCC
>Mature Secondary Structure
MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNPIGTDAVKVMEAIESVA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
DADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVEGTLAATVSAASGADIDKV
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCHHHH
IFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSVVIKNRNGLHVRPASRLVYTL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCEEECCHHHEEEEE
STFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLIAKGPEAEEALIAFRQLAEDNFG
ECCCCEEEEECCCCEECCCCCCCEEEEEEEECCEEEEEECCCCHHHHHHHHHHHHCCCCC
ETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSILTVEEEQERLRQAIDFTLLDLMTLT
CCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHH
AKAETSGLDDIAAIFSGHHTLLDDPELQAAASELLQHEHCTAEYAWQHVLKELSQQYQQL
HHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDEYLQARYIDVDDLLHRTQVHLTQTKEELPQFNSPTILLAENIYPSTVLQLDPAVVKGI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHEEECHHHHHHH
CLSAGSPLSHSALIARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG
HHCCCCCCCHHHHHHHHCCCCEEECCCCEEEEECCCCEEEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2671658; 7984428 [H]