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The map label for this gene is rspB

Identifier: 218699854

GI number: 218699854

Start: 1554589

End: 1555608

Strand: Direct

Name: rspB

Synonym: ECIAI39_1478

Alternate gene names: 218699854

Gene position: 1554589-1555608 (Clockwise)

Preceding gene: 218699853

Following gene: 218699856

Centisome position: 30.29

GC content: 45.49

Gene sequence:

>1020_bases
ATGAAAAGCATTTTAATTGAAAAACCGAATCAACTGGCAATTATCGAACGCGAAATACCCACCCCGTCAGCGGGTGAAGT
ACGAGTAAAAGTGAAACTTGCCGGAATTTGTGGTTCAGATAGCCATATTTATCGTGGGCATAATCCTTTTGCGAAATATC
CGCGCGTCATTGGACATGAATTCTTTGGCGTCATTGATGCAGTGGGTGAAGGCGTGGAAAGCGACAGAGTCGGTGAACGC
GTTGCTGTCGATCCGGTGGTCAGCTGTGGGCATTGCTATCCGTGCTCTATAGGTAAGCCGAACGTTTGTACGACACTGGC
TGTATTAGGTGTGCACGCTGACGGTGGTTTCAGTGAATATGCCGTGGTGCCGGCAAAAAATGCGTGGAAAATTCCTGAAG
CAGTGGCCGATCAATATGCGGTGATGATTGAACCTTTTACCATTGCGGCTAACGTTACCGGTCATGGTCAACCGACTGAA
AATGATACCGTTCTGGTTTACGGTGCAGGTCCAATCGGCCTGACGATCGTTCAGGTATTAAAAGGCGTCTATAACGTTAA
GAATGTGATTGTTGCCGATCGCATTGATGAACGACTGGAAAAAGCGAAAGAGAGCGGGGCAGACTGGGCGATTAATAACA
GCCAGACACCGCTTGGCGAGAGTTTCGCTGAAAAAGGCATCAAGCCGACTTTAATTATCGATGCCGCTTGTCATCCTTCT
ATCTTGAAAGAGGCCGTAACGCTGGCTTCTCCAGCGGCACGTATTGTATTGATGGGCTTCTCCAGTGAACCGTCTGAAGT
GATTCAGCAAGGAATTACCGGAAAAGAACTCTCTATTTTCTCTTCACGCTTAAATGCAAATAAATTCCCGGTCGTTATCG
ACTGGTTAAGTAAAGGGTTAATTAAACCAGAAAAACTAATTACCCATACGTTTGATTTCCAGCATGTTGCTGATGCCATT
AGTTTATTTGAACAGGATCAAAAGCATTGCTGCAAAGTCTTACTCACTTTTTCTGAATAA

Upstream 100 bases:

>100_bases
TCGATGAAAAGCTGGCGGCGAAATATCCCTATGAACCTGCTTATCTGCCAGTCGCACGTCTGGAAGATGGCACGCTGTGG
AACTGGTAAGGAGTAAGATA

Downstream 100 bases:

>100_bases
TACCAATAACGGCGAGTAAGTAGTACGCATCTTACCTCTTTTTTAGAGATAACCATTATGACAATAGAAAAACACGAAAG
AAGCACTAAGGATTTGGTGA

Product: putative dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHEFFGVIDAVGEGVESDRVGER
VAVDPVVSCGHCYPCSIGKPNVCTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGESFAEKGIKPTLIIDAACHPS
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAI
SLFEQDQKHCCKVLLTFSE

Sequences:

>Translated_339_residues
MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHEFFGVIDAVGEGVESDRVGER
VAVDPVVSCGHCYPCSIGKPNVCTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGESFAEKGIKPTLIIDAACHPS
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAI
SLFEQDQKHCCKVLLTFSE
>Mature_339_residues
MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHEFFGVIDAVGEGVESDRVGER
VAVDPVVSCGHCYPCSIGKPNVCTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGESFAEKGIKPTLIIDAACHPS
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAI
SLFEQDQKHCCKVLLTFSE

Specific function: Not known; probable catabolic enzyme [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=333, Percent_Identity=28.8288288288288, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI262073058, Length=375, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI71743840, Length=375, Percent_Identity=26.6666666666667, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI156523966, Length=372, Percent_Identity=24.7311827956989, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI4501939, Length=364, Percent_Identity=24.4505494505494, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4501929, Length=367, Percent_Identity=25.8855585831063, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4501933, Length=377, Percent_Identity=26.525198938992, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI71565154, Length=358, Percent_Identity=25.6983240223464, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI34577061, Length=377, Percent_Identity=25.7294429708223, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1787863, Length=339, Percent_Identity=98.5250737463127, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI226510992, Length=347, Percent_Identity=31.700288184438, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1790045, Length=318, Percent_Identity=30.188679245283, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788407, Length=333, Percent_Identity=31.8318318318318, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788073, Length=345, Percent_Identity=27.2463768115942, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790718, Length=348, Percent_Identity=27.5862068965517, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87082125, Length=366, Percent_Identity=28.1420765027322, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788075, Length=340, Percent_Identity=27.9411764705882, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786825, Length=394, Percent_Identity=23.8578680203046, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081918, Length=269, Percent_Identity=29.368029739777, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786552, Length=352, Percent_Identity=27.8409090909091, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17562876, Length=331, Percent_Identity=29.3051359516616, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17562878, Length=208, Percent_Identity=32.2115384615385, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25146526, Length=360, Percent_Identity=26.1111111111111, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71997431, Length=362, Percent_Identity=25.9668508287293, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17565904, Length=217, Percent_Identity=29.4930875576037, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=339, Percent_Identity=25.0737463126844, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562584, Length=344, Percent_Identity=23.2558139534884, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322619, Length=349, Percent_Identity=27.2206303724928, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6319955, Length=349, Percent_Identity=27.2206303724928, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323099, Length=341, Percent_Identity=26.6862170087977, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319258, Length=222, Percent_Identity=31.0810810810811, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=347, Percent_Identity=25.6484149855908, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=212, Percent_Identity=28.7735849056604, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=134, Percent_Identity=33.5820895522388, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=317, Percent_Identity=25.8675078864353, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=133, Percent_Identity=32.3308270676692, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=134, Percent_Identity=32.089552238806, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137530, Length=323, Percent_Identity=27.2445820433437, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17737897, Length=333, Percent_Identity=26.4264264264264, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45551930, Length=323, Percent_Identity=22.2910216718266, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550770, Length=323, Percent_Identity=22.2910216718266, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221457811, Length=323, Percent_Identity=22.2910216718266, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17737895, Length=201, Percent_Identity=27.363184079602, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 36552; Mature: 36552

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHE
CCCCEEECCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCCEECCCCCHHHHHHHHHHH
FFGVIDAVGEGVESDRVGERVAVDPVVSCGHCYPCSIGKPNVCTTLAVLGVHADGGFSEY
HHHHHHHHCCCCCCHHCCCEEEECHHHHCCCEEECCCCCCCHHHEEEEEEEECCCCCCCE
AVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTENDTVLVYGAGPIGLTIVQVL
EEEECCCCCCCCHHHHCCEEEEEECEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHH
KGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGESFAEKGIKPTLIIDAACHPS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHCCCCCEEEEECCCCHH
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFSSRLNANKFPVVIDWLSKGL
HHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEHHHCCC
IKPEKLITHTFDFQHVADAISLFEQDQKHCCKVLLTFSE
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIYRGHNPFAKYPRVIGHE
CCCCEEECCCCEEEEEECCCCCCCCCEEEEEEEEEEECCCCCEECCCCCHHHHHHHHHHH
FFGVIDAVGEGVESDRVGERVAVDPVVSCGHCYPCSIGKPNVCTTLAVLGVHADGGFSEY
HHHHHHHHCCCCCCHHCCCEEEECHHHHCCCEEECCCCCCCHHHEEEEEEEECCCCCCCE
AVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTENDTVLVYGAGPIGLTIVQVL
EEEECCCCCCCCHHHHCCEEEEEECEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHH
KGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPLGESFAEKGIKPTLIIDAACHPS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHCCCCCEEEEECCCCHH
ILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKELSIFSSRLNANKFPVVIDWLSKGL
HHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEHHHCCC
IKPEKLITHTFDFQHVADAISLFEQDQKHCCKVLLTFSE
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]