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The map label for this gene is rspA

Identifier: 218699853

GI number: 218699853

Start: 1553363

End: 1554577

Strand: Direct

Name: rspA

Synonym: ECIAI39_1477

Alternate gene names: 218699853

Gene position: 1553363-1554577 (Clockwise)

Preceding gene: 218699852

Following gene: 218699854

Centisome position: 30.27

GC content: 51.03

Gene sequence:

>1215_bases
ATGAAGATCGTAAAGGCTGAAGTTTTTGTTACCTGTCCGGGGCGTAATTTCGTCACATTAAAAATCACCACTGAGGACGG
TATTACGGGCCTTGGGGATGCCACCCTCAATGGACGTGAGCTTTCCGTGGCCTCTTATTTGCAGGATCACCTTTGTCCGC
AGCTTATTGGTCGCGATGCGCACCGTATCGAAGATATCTGGCAGTTTTTCTATAAAGGTGCTTACTGGCGTCGCGGTCCG
GTTACGATGTCGGCCATTTCAGCGGTTGATATGGCGCTGTGGGATATTAAAGCCAAAGCTGCCAACATGCCGCTTTACCA
GTTACTCGGCGGCGCGTCTCGTGAAGGGGTGATGGTTTATTGCCATACCACCGGTCACAGTATTGATGAAGCTCTGGATG
ATTATGCCCGTCATCAGGAGCTGGGATTCAAAGCCATCCGCGTGCAGTGCGGAATCCCTGGTATGAAAACCACCTATGGC
ATGTCGAAAGGTAAAGGTCTGGCTTATGAACCCGCAACCAAAGGACAGTGGCCGGAAGAGCAGCTGTGGTCGACGGAGAA
ATACCTCGATTTCATGCCGAAATTGTTTGACGCGGTACGTAACAAGTTTGGTTTTAATGAACATTTGCTGCATGACATGC
ACCATCGCTTAACGCCTATTGAAGCGGCGCACTTTGGTAAAAGCATTGAAGATTATCGCATGTTCTGGATGGAAGACCCG
ACGCCTGCGGAAAACCAGGAGTGTTTCCGCCTGATTCGCCAACACACCGTCACGCCAATTGCAGTGGGTGAAGTCTTCAA
CAGCATCTGGGACTGCAAACAGCTGATTGAAGAACAACTTATCGACTATATCCGCACCACGCTGACTCACGCGGGGGGGA
TTACCGGTATGCGCCGGATTGCCGATTTTGCTTCGTTGTATCAGGTACGTACTGGCTCACACGGTCCTTCCGATTTGTCA
CCAGTCTGCATGGCTGCGGCGCTGCACTTTGATCTGTGGGTCCCCAATTTCGGTGTCCAGGAGTACATGGGTTATTCCGA
ACAAATGCTTGAAGTCTTCCCGCACAACTGGACTTTCGATAACGGCTATATGCATCCGGGAGACAAACCGGGTCTTGGCA
TCGAATTCGATGAAAAGCTGGCGGCGAAATATCCCTATGAACCTGCTTATCTGCCAGTCGCACGTCTGGAAGATGGCACG
CTGTGGAACTGGTAA

Upstream 100 bases:

>100_bases
TTTGTGATCACTCCGGCTTTTTTTGATCTTCATGCTTGTATGGTAGTAGCTCAGTTGCGTAGATTTCATGCATCACGACA
AGCGATGCAAGGAATCGAAC

Downstream 100 bases:

>100_bases
GGAGTAAGATAATGAAAAGCATTTTAATTGAAAAACCGAATCAACTGGCAATTATCGAACGCGAAATACCCACCCCGTCA
GCGGGTGAAGTACGAGTAAA

Product: putative enolase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGP
VTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAAHFGKSIEDYRMFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGT
LWNW

Sequences:

>Translated_404_residues
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGP
VTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAAHFGKSIEDYRMFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGT
LWNW
>Mature_404_residues
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGP
VTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAAHFGKSIEDYRMFWMEDP
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLS
PVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGT
LWNW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=99.7524752475248, Blast_Score=847, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=414, Percent_Identity=30.4347826086957, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45950; Mature: 45950

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPQLIGRDA
CCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCH
HRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVY
HHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
CHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEE
EECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCCCCCEECCCCCCCCCHH
QLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAAHFGKSIEDYRMFWMEDP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCHHHHEEEEECCC
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHCCCCCCCC
NGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNW
CCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVASYLQDHLCPQLIGRDA
CCEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCH
HRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAANMPLYQLLGGASREGVMVY
HHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEE
CHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYGMSKGKGLAYEPATKGQWPEE
EECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHCCCCCCCCCEECCCCCCCCCHH
QLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLTPIEAAHFGKSIEDYRMFWMEDP
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCHHHHEEEEECCC
TPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTLTHAGGITGMRRI
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHNWTFD
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCHHHHHHHHCCCCCCCC
NGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARLEDGTLWNW
CCEECCCCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]