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The map label for this gene is ynfM

Identifier: 218699838

GI number: 218699838

Start: 1537553

End: 1538806

Strand: Reverse

Name: ynfM

Synonym: ECIAI39_1462

Alternate gene names: 218699838

Gene position: 1538806-1537553 (Counterclockwise)

Preceding gene: 218699842

Following gene: 218699837

Centisome position: 29.98

GC content: 53.43

Gene sequence:

>1254_bases
GTGAGCCGTACTACAACTGTTGATGGCGCTCCGGCAAGCGACACTGACAAGCAAAGCATTTCTCAGCCAAATCAATTTAT
TAAACGCGGTACGCCGCAATTTATGCGCGTCACCCTGGCGCTGTTCTCTGCCGGACTGGCAACCTTCGCACTTCTCTATT
GTGTGCAACCTATCCTTCCGGTGCTTTCGCAGGAGTTTGGCTTAACGCCCGCGAACAGTAGTATTTCACTGTCCATTTCC
ACGGCGATGTTGGCTATCGGTTTGCTGTTTACTGGCCCGCTATCCGATGCCATTGGTCGCAAACCAGTGATGGTCACGGC
GCTACTGTTGGCCTCCATTTGTACGTTACTTTCGACAATGATGACCAGCTGGCACGGCATTTTGATTATGCGCGCCTTGA
TTGGGCTTTCGTTAAGTGGCGTGGCAGCCGTTGGCATGACTTATCTTAGCGAGGAAATCCACCCCAGTTTCGTGGCCTTT
TCGATGGGGTTGTATATCAGCGGCAACTCAATTGGTGGCATGAGCGGACGCTTAATTAGCGGTGTCTTCACGGACTTTTT
CAACTGGCGAATTGCGCTGGCGGCAATCGGTTGTTTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCGGAAT
CACGCCATTTTCGCCCGACTTCGCTGCGACCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGACCGGGGATTA
CCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCT
CTCCCCCTGGCATGTCAGTCAGGCTGTGGTTGGCTTATTATCGCTGGCCTATTTGACCGGTACATGGAGCTCACCCAAAG
CCGGAACCATGACCACCCGCTATGGGCGCGGTCCGGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATG
ACCTTATTTAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCCGGATTCTTCGCAGCCCACTCAGTAGC
CAGCAGCTGGATCGGCCCCCGAGCAAAACGCGCTAAAGGCCAGGCCTCCTCGCTGTATCTGTTCAGTTACTATCTGGGGT
CGAGTATTGCCGGGACGCTGGGTGGTGTTTTCTGGCATAACTATGGCTGGAACGGCGTCGGCGCATTTATTGCTCTGATG
CTGGTCATTGCTCTGCTGGTCGGGACGCGTTTGCATCGTCGTCTGCACGCCTGA

Upstream 100 bases:

>100_bases
AACATCTTATTTGAGATTATTAATATATTAGACAGAACAATTCGATTTTCCTAATCTATCCATAAGCCTGATCCACAGGC
ATATTTAGCAAGGATTTCAA

Downstream 100 bases:

>100_bases
AAAATAAGTCCGGACTGCGGTAAATACCCATCCGGACTTATTGCCTGCTCAAACCAACGTTAATAGCCATCCTAAAATAG
ACGAAGCGCCAGCCAATTCC

Product: putative MFS superfamily transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSAGLATFALLYCVQPILPVLSQEFGLTPANSSISLSIS
TAMLAIGLLFTGPLSDAIGRKPVMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFRPTSLRPKTLFINFRLHWRDRGL
PLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAVVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLM
TLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNGVGAFIALM
LVIALLVGTRLHRRLHA

Sequences:

>Translated_417_residues
MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSAGLATFALLYCVQPILPVLSQEFGLTPANSSISLSIS
TAMLAIGLLFTGPLSDAIGRKPVMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFRPTSLRPKTLFINFRLHWRDRGL
PLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAVVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLM
TLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNGVGAFIALM
LVIALLVGTRLHRRLHA
>Mature_416_residues
SRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSAGLATFALLYCVQPILPVLSQEFGLTPANSSISLSIST
AMLAIGLLFTGPLSDAIGRKPVMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAFS
MGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFRPTSLRPKTLFINFRLHWRDRGLP
LLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAVVGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMT
LFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNGVGAFIALML
VIALLVGTRLHRRLHA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily

Homologues:

Organism=Escherichia coli, GI1787880, Length=417, Percent_Identity=100, Blast_Score=826, Evalue=0.0,
Organism=Escherichia coli, GI87082404, Length=176, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YNFM_ECOLI (P43531)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   AF405541
- PIR:   F64915
- RefSeq:   AP_002217.1
- RefSeq:   NP_416113.1
- ProteinModelPortal:   P43531
- STRING:   P43531
- EnsemblBacteria:   EBESCT00000003203
- EnsemblBacteria:   EBESCT00000015541
- GeneID:   946138
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1588
- KEGG:   eco:b1596
- EchoBASE:   EB2785
- EcoGene:   EG12951
- eggNOG:   COG0477
- GeneTree:   EBGT00050000008823
- HOGENOM:   HBG699254
- OMA:   IYIMANS
- ProtClustDB:   CLSK880142
- BioCyc:   EcoCyc:B1596-MONOMER
- Genevestigator:   P43531
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 45258; Mature: 45127

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2193073c)-; HASH(0x2187895c)-; HASH(0x20cce294)-; HASH(0x21a13038)-; HASH(0x21fa8164)-; HASH(0x21b9c658)-; HASH(0x213a3530)-; HASH(0x21cefa6c)-; HASH(0x2162d388)-; HASH(0x21f8cec0)-; HASH(0x21a169a0)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSAGLATFALLYCVQPILP
CCCCCCCCCCCCCCCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKPVMVTALLLASICTLLSTM
HHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
MTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAFSMGLYISGNSIGGMSGRLIS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHCEEEECCCCCCCCHHHHH
GVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFRPTSLRPKTLFINFRLHWRDRGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCC
PLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAVVGLLSLAYLTGTWSSPKAGTMTTR
CEEHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
QASSLYLFSYYLGSSIAGTLGGVFWHNYGWNGVGAFIALMLVIALLVGTRLHRRLHA
CCCCEEHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSAGLATFALLYCVQPILP
CCCCCCCCCCCCCCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKPVMVTALLLASICTLLSTM
HHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHH
MTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAFSMGLYISGNSIGGMSGRLIS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHCEEEECCCCCCCCHHHHH
GVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFRPTSLRPKTLFINFRLHWRDRGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCC
PLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAVVGLLSLAYLTGTWSSPKAGTMTTR
CEEHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
YGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGMLLFSAGFFAAHSVASSWIGPRAKRAKG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCC
QASSLYLFSYYLGSSIAGTLGGVFWHNYGWNGVGAFIALMLVIALLVGTRLHRRLHA
CCCCEEHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503