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The map label for this gene is pntB

Identifier: 218699832

GI number: 218699832

Start: 1532145

End: 1533533

Strand: Direct

Name: pntB

Synonym: ECIAI39_1456

Alternate gene names: 218699832

Gene position: 1532145-1533533 (Clockwise)

Preceding gene: 218699831

Following gene: 218699834

Centisome position: 29.85

GC content: 52.34

Gene sequence:

>1389_bases
ATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCGATCCTGTTTATCTTCAGTCTGGCCGGTCTTTCGAAACA
TGAAACGTCTCGCCAGGGTAACAACTTCGGTATCGCCGGGATGGCGATTGCGTTAATCGCAACCATTTTTGGACCGGATA
CGGGTAATGTTGGCTGGATCTTGCTGGCTATGGTCATTGGTGGGGCAATTGGTATCCGGCTGGCGAAAAAAGTTGAAATG
ACCGAAATGCCAGAACTGGTGGCGATCCTGCATAGCTTCGTGGGTCTGGCGGCAGTGCTGGTTGGCTTTAACAGCTATCT
GCATCATGACGCGGGAATGGCACCGATTCTGGTCAATATTCACCTGACGGAAGTGTTCCTCGGTATCTTCATCGGGGCGG
TAACGTTCACGGGTTCGGTGGTGGCGTTCGGCAAACTGTGTGGCAAGATTTCGTCTAAACCGTTGATGCTGCCAAACCGT
CACAAAATGAACCTGGCGGCTCTGGTCGTTTCCTTCCTGCTGCTGATTGTATTTGTTCGCACGGACAGCGTCGGCCTGCA
AGTGCTGGCATTGCTGATAATGACCGCCATTGCGCTGGTATTTGGCTGGCATTTGGTCGCCTCTATTGGTGGTGCAGATA
TGCCAGTGGTGGTATCAATGCTGAACTCGTACTCCGGCTGGGCGGCTGCGGCTGCGGGCTTTATGCTCAGCAACGACCTG
CTGATTGTGACCGGTGCGCTGGTCGGTTCTTCGGGGGCTATCCTTTCTTACATTATGTGTAAGGCGATGAACCGTTCTTT
TATCAGCGTTATTGCGGGTGGTTTCGGCACCGACGGCTCTTCTACTGGCGATGATCAGGAAGTGGGCGAGCACCGCGAAA
TCACTGCAGAAGAGACAGCGGAACTGCTGAAAAACTCCCATTCAGTGATCATTACTCCGGGGTACGGCATGGCAGTCGCG
CAGGCGCAATATCCTGTCGCTGAAATTACTGAGAAATTGCGCGCTCGTGGTATTAATGTGCGTTTCGGTATCCACCCGGT
TGCGGGGCGTTTGCCTGGACATATGAACGTATTGCTGGCTGAAGCAAAAGTACCGTATGACATCGTGCTGGAAATGGACG
AGATCAACGATGACTTTGCTGATACCGATACCGTACTGGTGATTGGTGCTAACGATACGGTTAACCCGGCGGCGCAGGAT
GATCCGAAGAGTCCGATTGCTGGTATGCCTGTGCTGGAAGTGTGGAAAGCGCAGAACGTGATTGTCTTTAAACGTTCGAT
GAACACTGGCTATGCTGGTGTGCAAAACCCGCTGTTCTTCAAGGAAAACACCCACATGCTGTTTGGTGACGCCAAAGCCA
GCGTGGATGCAATCCTGAAAGCTCTGTAA

Upstream 100 bases:

>100_bases
AGTTTTATCGCGGTGCTTATAGCCAGCATTAATATTTTCGGTGGCTTCACCGTGACTCAGCGCATGCTGAAAATGTTCCG
CAAAAATTAAGGGGTAACAT

Downstream 100 bases:

>100_bases
CCCTGACGGCCTCTGCGGAGGCCGTCACTCTTTATTGAGATCGCTTAACAGAACGGCGATGCTTTGACCTCCCGCTGTTT
GTTCAAGTGCAATTTTGACA

Product: pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Pyridine nucleotide transhydrogenase subunit beta

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIALIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEM
TEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVA
QAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQD
DPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL

Sequences:

>Translated_462_residues
MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIALIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEM
TEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVA
QAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQD
DPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL
>Mature_461_residues
SGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIALIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMT
EMPELVAILHSFVGLAAVLVGFNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNRH
KMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLL
IVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQ
AQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQDD
PKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family

Homologues:

Organism=Homo sapiens, GI122939155, Length=456, Percent_Identity=52.1929824561403, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI122939153, Length=456, Percent_Identity=52.1929824561403, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI1787886, Length=462, Percent_Identity=100, Blast_Score=927, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=457, Percent_Identity=49.45295404814, Blast_Score=427, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNTB_ECO57 (P0AB69)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85766
- PIR:   D90917
- RefSeq:   NP_288037.1
- RefSeq:   NP_310335.1
- ProteinModelPortal:   P0AB69
- SMR:   P0AB69
- EnsemblBacteria:   EBESCT00000026505
- EnsemblBacteria:   EBESCT00000055414
- GeneID:   913582
- GeneID:   961550
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2597
- KEGG:   ecs:ECs2308
- GeneTree:   EBGT00050000011496
- HOGENOM:   HBG648813
- OMA:   VEIYVGI
- ProtClustDB:   PRK09444
- BioCyc:   ECOL83334:ECS2308-MONOMER
- InterPro:   IPR012136
- InterPro:   IPR004003
- PIRSF:   PIRSF000204

Pfam domain/function: PF02233 PNTB

EC number: =1.6.1.2

Molecular weight: Translated: 48724; Mature: 48593

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x21a9ca68)-; HASH(0x21fbdb38)-; HASH(0x21806fe4)-; HASH(0x21c62a4c)-; HASH(0x21ba80b4)-; HASH(0x20dffa04)-; HASH(0x200ccd3c)-; HASH(0x21c62968)-; HASH(0x21f81b04)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIALIATIFGPDTGNVGWI
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
LLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMAPILVNI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNRHKMNLAALVVSFLLLIVFVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCE
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGSSTGDDQEVGEHREITAEETA
EEEEEEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHH
ELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLA
HHHHCCCCEEEECCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEEE
EAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQDDPKSPIAGMPVLEVWKAQNV
CCCCCEEEEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCE
IVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL
EEEEECCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHCH
>Mature Secondary Structure 
SGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIALIATIFGPDTGNVGWI
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
LLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVGFNSYLHHDAGMAPILVNI
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNRHKMNLAALVVSFLLLIVFVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCE
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGSSTGDDQEVGEHREITAEETA
EEEEEEHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHH
ELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLRARGINVRFGIHPVAGRLPGHMNVLLA
HHHHCCCCEEEECCCCCCHHHCCCCHHHHHHHHHHCCCEEEECCCHHCCCCCCCCEEEEE
EAKVPYDIVLEMDEINDDFADTDTVLVIGANDTVNPAAQDDPKSPIAGMPVLEVWKAQNV
CCCCCEEEEEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCE
IVFKRSMNTGYAGVQNPLFFKENTHMLFGDAKASVDAILKAL
EEEEECCCCCCCCCCCCEEEECCCEEEEECCHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796