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The map label for this gene is pntA

Identifier: 218699831

GI number: 218699831

Start: 1530602

End: 1532134

Strand: Direct

Name: pntA

Synonym: ECIAI39_1455

Alternate gene names: 218699831

Gene position: 1530602-1532134 (Clockwise)

Preceding gene: 218699827

Following gene: 218699832

Centisome position: 29.82

GC content: 52.71

Gene sequence:

>1533_bases
ATGCGAATTGGCATACCAAGAGAACGGTTAACCAATGAAACCCGTGTTGCAGCAACGCCAAAAACAGTGGAACAGCTGCT
GAAACTGGGTTTTACCGTCGCGGTAGAGAGCGGCGCGGGTCAACTGGCAAGTTTTGACGATAAAGCGTTTGTGCAAGCGG
GCGCTGAAATTGTAGAAGGGAATAGCGTCTGGCAGTCAGAGATCATTCTGAAGGTCAATGCGCCGTTAGATGATGAAATT
GCGTTACTGAATCCTGGGACAACGCTGGTGAGTTTTATCTGGCCTGCGCAGAATCCGGAATTAATGCAAAAACTTGCGGA
ACGTAACGTGACCGTGATGGCGATGGACTCTGTGCCGCGTATCTCACGCGCACAATCGCTGGACGCACTAAGCTCGATGG
CGAACATCGCCGGTTATCGCGCCATTGTTGAAGCGGCACATGAATTTGGGCGTTTCTTTACCGGGCAAATTACTGCGGCC
GGGAAAGTGCCACCGGCAAAAGTGATGGTGATTGGTGCGGGTGTTGCAGGTCTGGCCGCCATTGGCGCAGCAAACAGTCT
CGGCGCGATTGTGCGTGCATTCGACACCCGCCCGGAAGTGAAAGAACAAGTTCAAAGTATGGGCGCGGAATTCCTCGAGC
TGGATTTTAAAGAGGAAGCGGGCAGCGGCGATGGCTATGCCAAAGTGATGTCGGACGCGTTCATCAAAGCGGAAATGGAA
CTCTTTGCCGCCCAGGCAAAAGAGGTCGATATCATTGTCACCACCGCGCTTATTCCAGGCAAACCAGCGCCGAGGCTAAT
TACCCGTGAAATGGTTGACTCCATGAAGGCGGGCAGTGTGATTGTCGACCTGGCAGCCCAAAACGGCGGCAACTGTGAAT
ACACCGTGCCGGGTGAAATCTTCACTACGGAAAATGGTGTCAAAGTGATTGGTTATACCGATCTTCCGGGCCGTCTGCCG
ACGCAATCCTCACAGCTTTACGGCACTAACCTCGTTAATCTGCTGAAACTGTTGTGCAAAGAGAAAGACGGCAACATCAC
TGTGGATTTTGATGATGTGGTGATTCGCGGCGTGACCGTGATCCGTGCGGGCGAAATTACCTGGCCGGCACCGCCGATTC
AGGTATCAGCTCAGCCGCAGGCGGCACAAAAAGCGGCACCGGAAGTGAAAACTGAGGAAAAATGTGCCTGCTCACCATGG
CGTAAATACGCGTTGATGGCGTTGGCAATCATCCTTTTCGGCTGGATGGCAAGCGTTGCGCCAAAAGAGTTCCTTGGGCA
CTTCACTGTGTTCGCGCTGGCCTGCGTTGTCGGTTATTACGTGGTGTGGAATGTATCGCACGCGCTGCATACACCGTTGA
TGTCGGTCACCAACGCGATTTCAGGGATTATTGTTGTCGGAGCACTGTTGCAGATTGGCCAGGGCGGCTGGGTTAGCTTC
CTTAGTTTTATCGCGGTGCTTATAGCCAGCATTAATATTTTCGGTGGCTTCACCGTGACTCAGCGCATGCTGAAAATGTT
CCGCAAAAATTAA

Upstream 100 bases:

>100_bases
ATTTATTTTAACGGAGTAACATTTAGCTCGTACATGAGCGGCTTGTGTGGCTCCTGACACAGGCAAACCATCATCAATAA
AACCGATGGAAGGGAATATC

Downstream 100 bases:

>100_bases
GGGGTAACATATGTCTGGAGGATTAGTTACAGCTGCATACATTGTTGCCGCGATCCTGTTTATCTTCAGTCTGGCCGGTC
TTTCGAAACATGAAACGTCT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEI
ALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
LFAAQAKEVDIIVTTALIPGKPAPRLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCACSPW
RKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_510_residues
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEI
ALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
LFAAQAKEVDIIVTTALIPGKPAPRLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCACSPW
RKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_510_residues
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEI
ALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
LFAAQAKEVDIIVTTALIPGKPAPRLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLP
TQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCACSPW
RKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
LSFIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=532, Percent_Identity=50.3759398496241, Blast_Score=498, Evalue=1e-141,
Organism=Homo sapiens, GI122939153, Length=532, Percent_Identity=50.3759398496241, Blast_Score=498, Evalue=1e-141,
Organism=Escherichia coli, GI1787887, Length=510, Percent_Identity=99.6078431372549, Blast_Score=1035, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=520, Percent_Identity=50.5769230769231, Blast_Score=483, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54622; Mature: 54622

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEG
CCCCCCHHHHCCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHCHHHCCC
NSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPR
CCEEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCCHHHHHHHHHHHHHHH
LFAAQAKEVDIIVTTALIPGKPAPRLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEI
HHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCEE
FTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTV
EECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHCEEE
IRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCACSPWRKYALMALAIILFGWMASVA
EEECCEECCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEG
CCCCCCHHHHCCCCEEEECHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHCHHHCCC
NSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPR
CCEEEEEEEEEECCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCC
ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME
HCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEECHHCCCCCCCHHHHHHHHHHHHHHH
LFAAQAKEVDIIVTTALIPGKPAPRLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEI
HHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCEE
FTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTV
EECCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHCEEE
IRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCACSPWRKYALMALAIILFGWMASVA
EEECCEECCCCCCEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LSFIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]