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The map label for this gene is malI

Identifier: 218699813

GI number: 218699813

Start: 1508066

End: 1509094

Strand: Direct

Name: malI

Synonym: ECIAI39_1437

Alternate gene names: 218699813

Gene position: 1508066-1509094 (Clockwise)

Preceding gene: 218699809

Following gene: 218699814

Centisome position: 29.39

GC content: 54.23

Gene sequence:

>1029_bases
ATGGCTACCGCCAAAAAAATAACCATTCATGATGTTGCGCTGGCTGCGGGCGTGTCGGTAAGCACCGTTTCGCTGGTGCT
AAGTGGCAAAGGGCGAATCTCTACCGCCACAGGAGAACGCGTTAACGCCGCCATTGAAGAGCTGGGATTTGTACGCAATC
GTCAGGCGTCGGCGCTGCGCGGCGGGCAAAGCGGCGTCATTGGTTTGATCGTCCGTGATTTATCTGCGCCGTTTTACGCC
GAATTAACGGCCGGATTGACGGAAGCTCTGGAAGCGCAGGGACGGATGGTTTTTTTGCTTCACGGCGGTAAAGACGGCGA
GCAGCTGGCACAACGGTTTTCACTGTTACTGAATCAGGGGGTCGATGGTGTGGTCATTGCCGGGGCTGCAGGAAGCAGCG
ATGACCTGCGACGGATGGCAGAAGAAAAAGCTATCCCGGTGATTTTCGCTTCCCGTGCCAGTTATCTTGATGATGTTGAT
ACGGTTCGCCCGGACAACATGCAGGCAGCACAGTTGTTAACGGAGCATCTCATTCGCAATGGGCATCAGCGTATCGCCTG
GCTGGGAGGGCAAAGTTCCTCATTAACCCGGGCAGAGCGGGTGGGGGGCTATTGTGCAACTCTACTAAAATTTGGCCTGC
CGTTTCACAGCGATTGGGTGTTGGAGTGCACTTCCAGCCAGAAGCAAGCCGCGGAAGCTATCACGGCGCTTTTACGTCAT
AACCCGACCATCAGTGCTGTGGTTTGCTATAACGAAACTATTGCGATGGGGGCATGGTTTGGTTTGCTGAAAGCAGGGCG
GCAAAGCGGGGAAAGCGGAGTCGATCGTTACTTTGAACAACAGGTTTCGTTGGCGGCATTTACCGATGCAACGCCAACCA
CACTTGATGATATACCTGTTACCTGGGCCAGCACGCCTGCGCGGGAACTTGGTACCACACTTGCGGATCGCATGATGCAA
AAAATCACCCATGAAGAGACGCATTCACGCAATCTTATTATTCCCGCCCGGCTCATTGCGGCGAAATAA

Upstream 100 bases:

>100_bases
GTGAGGCATAAATCACATTACGCAACGATAATAGCGGGTATAAGATAAATAAAAGGTAAAACGTTTTATCTGTCACATAA
TCAGGGAGTAGGTCATCTGC

Downstream 100 bases:

>100_bases
TCCTCTCTTTATCTGCTATACCTGGTAGTGTCCTTTCCTCAAGGTTAATGATAAAAAATGTGGCACACAGGCGGTTAATG
CCGATCAGTTAAGGATCAGT

Product: DNA-binding transcriptional repressor MalI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYA
ELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQ
KITHEETHSRNLIIPARLIAAK

Sequences:

>Translated_342_residues
MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYA
ELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQ
KITHEETHSRNLIIPARLIAAK
>Mature_341_residues
ATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAE
LTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDT
VRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRHN
PTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQK
ITHEETHSRNLIIPARLIAAK

Specific function: Repressor for the malX and malY genes. Also regulates its own expression. Binds maltose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787906, Length=342, Percent_Identity=99.7076023391813, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI1790194, Length=325, Percent_Identity=25.8461538461538, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI48994940, Length=330, Percent_Identity=27.8787878787879, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1789068, Length=317, Percent_Identity=29.0220820189274, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1790369, Length=305, Percent_Identity=26.2295081967213, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788474, Length=261, Percent_Identity=29.8850574712644, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=28.3737024221453, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786540, Length=343, Percent_Identity=26.2390670553936, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787580, Length=325, Percent_Identity=25.5384615384615, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=23.2835820895522, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790715, Length=334, Percent_Identity=24.8502994011976, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790689, Length=258, Percent_Identity=27.5193798449612, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786268, Length=187, Percent_Identity=23.5294117647059, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36613; Mature: 36482

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALR
CCCCCEEEHHHHHHHCCCCHHEEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCHHHHC
GGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCC
VDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDTVRPDNMQAAQLLTEHLIRN
CCEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHC
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPV
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
TWASTPARELGTTLADRMMQKITHEETHSRNLIIPARLIAAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCC
>Mature Secondary Structure 
ATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAAIEELGFVRNRQASALR
CCCCEEEHHHHHHHCCCCHHEEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCHHHHC
GGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHGGKDGEQLAQRFSLLLNQG
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCC
VDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVDTVRPDNMQAAQLLTEHLIRN
CCEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHC
GHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSDWVLECTSSQKQAAEAITALLRH
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHC
NPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDRYFEQQVSLAAFTDATPTTLDDIPV
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
TWASTPARELGTTLADRMMQKITHEETHSRNLIIPARLIAAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670898; 1856179; 9097039; 9278503 [H]