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The map label for this gene is manX

Identifier: 218699614

GI number: 218699614

Start: 1300722

End: 1301693

Strand: Reverse

Name: manX

Synonym: ECIAI39_1235

Alternate gene names: 218699614

Gene position: 1301693-1300722 (Counterclockwise)

Preceding gene: 218699616

Following gene: 218699613

Centisome position: 25.36

GC content: 50.1

Gene sequence:

>972_bases
GTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTGCTTAAAACGGCAGAAATGCTGTTAGGCGA
GCAGGAAAACGTCGGCTGGATCGATTTCGTTCCAGGTGAAAATGCCGAAACGCTGATTGAAAAGTACAACGCTCAGTTGG
CAAAACTCGACACCACTAAAGGCGTGCTGTTTCTCGTTGATACATGGGGAGGCAGCCCGTTCAATGCTGCCAGCCGCATT
GTCGTCGACAAAGAGCATTATGAAGTCATTGCAGGCGTTAACATTCCAATGCTCGTGGAAACGTTAATGGCCCGTGATGA
TGACCCAAGCTTTGATGAACTGGTTGCGCTGGCAGTAGAAACAGGCCGTGAAGGCGTGAAAGCTCTGAAAGCCAAACCGG
TTGAAAAAGCCGCGCCAGCACCCGCTGCCGTAGCACCAAAAGCGGCTCCAACTCCGGCAAAACCAATGGGACCAAACGAC
TACATGGTTATTGGCCTTGCGCGTATCGACGACCGTCTGATTCACGGTCAGGTCGCCACCCGCTGGACCAAAGAAACCAA
TGTCTCCCGTATTATTGTTGTTAGTGATGAAGTGGCTGCGGATACCGTTCGTAAGACACTGCTCACCCAGGTTGCACCTC
CGGGCGTAACAGCACACGTAGTTGATGTTGCCAAAATGATTCGCGTCTACAACAACCCGAAATATGCTGGCGAACGCGTA
ATGCTGTTATTTACCAACCCAACAGATGTAGAGCGTCTCGTTGAAGGCGGCGTGAAAATCACCTCTGTTAACGTCGGTGG
TATGGCATTCCGTCAGGGTAAAACCCAGGTGAATAACGCGGTTTCGGTTGATGAAAAAGATATCGAGGCGTTCAAGAAAC
TGAATGCGCGCGGTATTGAGCTGGAAGTCCGTAAGGTTTCCACCGATCCGAAACTGAAAATGATGGATCTGATCAGCAAA
ATCGATAAGTAA

Upstream 100 bases:

>100_bases
TTTTGGTTGTAGCCCTTATCTGAATCGATTCGATTGTGGACGACGATTCAAAAATACATCTGGCACGTTGAGGTGTTAAC
GATAATAAAGGAGGTAGCAA

Downstream 100 bases:

>100_bases
CGTATTGTGTTGATTATCACTCAGTTTTCACACTTAAGTCTTACGTAAACAGGAGAAGTACAATGGAGATTACCACTCTT
CAAATTGTGCTGGTATTTAT

Product: PTS system manniose-specific transporter subunit IIAB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRI
VVDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAVAPKAAPTPAKPMGPND
YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERV
MLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISK
IDK

Sequences:

>Translated_323_residues
MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRI
VVDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAVAPKAAPTPAKPMGPND
YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERV
MLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISK
IDK
>Mature_322_residues
TIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIV
VDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAVAPKAAPTPAKPMGPNDY
MVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVM
LLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKI
DK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=323, Percent_Identity=99.6904024767802, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=37.5796178343949, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789527, Length=152, Percent_Identity=33.5526315789474, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35076; Mature: 34945

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTK
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCHHEEEECCC
GVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVALAVE
CEEEEEECCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHH
TGREGVKALKAKPVEKAAPAPAAVAPKAAPTPAKPMGPNDYMVIGLARIDDRLIHGQVAT
HCHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCHHHCCHHHH
RWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERV
EEECCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
MLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNARGIE
EEEEECCHHHHHHHHCCEEEEEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCEE
LEVRKVSTDPKLKMMDLISKIDK
EEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTTK
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCHHEEEECCC
GVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETLMARDDDPSFDELVALAVE
CEEEEEECCCCCCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHH
TGREGVKALKAKPVEKAAPAPAAVAPKAAPTPAKPMGPNDYMVIGLARIDDRLIHGQVAT
HCHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCHHHCCHHHH
RWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERV
EEECCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
MLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNARGIE
EEEEECCHHHHHHHHCCEEEEEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHCCCCCEE
LEVRKVSTDPKLKMMDLISKIDK
EEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]