Click here to switch to the map view.

The map label for this gene is pphA

Identifier: 218699591

GI number: 218699591

Start: 1280772

End: 1281428

Strand: Direct

Name: pphA

Synonym: ECIAI39_1212

Alternate gene names: 218699591

Gene position: 1280772-1281428 (Clockwise)

Preceding gene: 218699590

Following gene: 218699597

Centisome position: 24.96

GC content: 47.95

Gene sequence:

>657_bases
ATGAAACAGACTGCGCCCGTTTATCAGAGAATTGCGGGTCATCAATGGCGACATATCTGGCTTTCAGGCGATATACACGG
TTGTCTTGAGCAGTTGCGCCGCAAATTATGGCATTGTCGTTTTGATCCGTGGCGAGATTTACTTATCTCAGTGGGAGACG
TTATCGATCGTGGGCAGCAAAGTTTACGTTGTCTGCAGTTACTGGAACAACATTGGGTTCGTGCGGTAAGAGGCAATCAT
GAACAGATGGCGATGGATGCGCTGGCATCCCGGCAGATGTCGTTGTGGTTGATGAATGGCGGCGACTGGTTTATTGCGCT
GGCAGATTATCAACAGAAACAAGCGAAAACGGCGCTGGAAAAATGTCAGTATTTGCCCTTTATTCTTGAAGTACACAGTC
GCACCGGCAAACATGTTATTGCCCATGCCGATTATCCGGATGATGTTTATGAATGGCAAAAGGACGTTGATTTGCATCAG
GTCTTGTGGAGCCGCTCGCGATTAGGTGAACGCCAAAAAGGGCAGGGAATTACAGGTGCTGATCATTTCTGGTTTGGTCA
TACACCGTTGCGCCATCGCGTGGATATTGGCAACCTGCATTATATTGATACAGGTGCTGTCTTTGGGGGCGAACTGACTG
TTGTGCAATTGCAATAA

Upstream 100 bases:

>100_bases
CTTTATAGTCAACCATAACACACACTCTACTGTCTGAGTCCAGCGTTTTTTAACATTCTTGTTAAGATTATGTGATCTTT
AGCGCGGGAGGAAAATATTG

Downstream 100 bases:

>100_bases
TTAAAAATCACCGTACTCCTGTGCAGGTCGCCAGAAACCATCTATAAAATCCTCAATCGGAAAACAACCGCCATGGCGGA
TCCGTTGATCGCTCATAGAA

Product: serine/threonine protein phosphatase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKQTAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGQQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASRQMSLWLMNGGDWFIALADYQQKQAKTALEKCQYLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTVVQLQ

Sequences:

>Translated_218_residues
MKQTAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGQQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASRQMSLWLMNGGDWFIALADYQQKQAKTALEKCQYLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTVVQLQ
>Mature_218_residues
MKQTAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGQQSLRCLQLLEQHWVRAVRGNH
EQMAMDALASRQMSLWLMNGGDWFIALADYQQKQAKTALEKCQYLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTVVQLQ

Specific function: Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr an

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=218, Percent_Identity=96.7889908256881, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1789090, Length=213, Percent_Identity=43.1924882629108, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 25451; Mature: 25451

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGQQ
CCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGDWFIALADYQQKQAKTALE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEECCCEEEEECCHHHHHHHHHHH
KCQYLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGITGA
HHHHCHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCCCC
DHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTVVQLQ
CCCEECCCCCHHEECCCCEEEEECCCEECCEEEEEEEC
>Mature Secondary Structure
MKQTAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGQQ
CCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLMNGGDWFIALADYQQKQAKTALE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEECCCEEEEECCHHHHHHHHHHH
KCQYLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGITGA
HHHHCHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCCCHHCCCCCCCC
DHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTVVQLQ
CCCEECCCCCHHEECCCCEEEEECCCEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9097040; 9278503 [H]