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The map label for this gene is fliF

Identifier: 218699502

GI number: 218699502

Start: 1193163

End: 1194821

Strand: Reverse

Name: fliF

Synonym: ECIAI39_1121

Alternate gene names: 218699502

Gene position: 1194821-1193163 (Counterclockwise)

Preceding gene: 218699504

Following gene: 218699499

Centisome position: 23.28

GC content: 54.91

Gene sequence:

>1659_bases
ATGAATGCGACTGCAGCCCAGACAAAATCTCTTGAGTGGCTTAATCGCCTGCGTGCGAATCCGAAAATTCCATTGATTGT
TGCCGGTTCCGCAGCAGTGGCGGTCATGGTCGCACTGATCCTGTGGGCGAAAGCCCCCGACTACCGCACATTATTCAGCA
ATCTTTCCGATCAGGATGGTGGCGCAATTGTCAGCCAACTGACGCAAATGAATATTCCTTACCGCTTCAGCGAAGCCAGC
GGCGCTATTGAGGTTCCGGCAGATAAAGTTCACGAACTGCGCCTGCGCCTGGCGCAACAAGGTTTGCCCAAAGGCGGCGC
GGTCGGTTTCGAACTGCTCGATCAGGAAAAGTTTGGTATCAGCCAGTTCAGCGAACAGGTGAATTATCAGCGGGCGCTGG
AAGGCGAGCTTTCTCGTACCATCGAAACTATCGGCCCGGTAAAAGGGGCGCGCGTACATCTGGCAATGCCGAAACCGTCT
TTATTCGTCCGTGAACAAAAATCCCCTTCTGCATCGGTGACGGTAAATCTGTTACCCGGCCGCGCACTCGATGAAGGGCA
AATTAGCGCTATTGTACATCTGGTTTCCAGCGCGGTTGCTGGTCTGCCGCCTGGAAACGTCACGCTGGTGGATCAGGGCG
GGCATCTGTTAACCCAGTCCAATACCAGCGGGCGCGATCTTAATGACGCTCAGTTGAAATATGCCAGCGATGTCGAAGGC
CGTATTCAGCGGCGTATTGAAGCGATCCTGTCGCCTATTGTTGGTAACGGTAATATTCACGCCCAGGTCACGGCGCAACT
GGACTTCGCCAGTAAAGAACAAACGGAAGAACAGTATCGCCCTAACGGTGATGAATCTCATGCGGCGCTTCGTTCACGCC
AGCTTAATGAGAGCGAGCAAAGCGGTTCCGGTTATCCGGGCGGCGTACCGGGGGCGTTGTCGAATCAACCGGCACCTGCG
AATAACGCGCCAATCAGCACGCCTCCGGCAAATCAAAATAACCGCCAGCAGCAGGCGAGCACCACCAGCAATAGTGGGCC
GCGTAGCACACAGCGGAATGAAACCAGTAACTACGAAGTCGATCGCACCATTCGTCATACCAAAATGAACGTGGGCGATG
TGCAACGTCTGTCAGTCGCGGTAGTGGTGAATTACAAAACCTTGCCAGACGGTAAACCATTGCCTCTCAGCAACGAACAG
ATGAAGCAAATTGAAGCTCTGACCCGCGAGGCGATGGGCTTTTCTGAAAAACGCGGTGATTCGCTCAATGTTGTTAACTC
GCCGTTCAATAGCAGTGACGAAAGCGGCGGAGCACTGCCGTTCTGGCAACAGCAAGTGTTTATCGATGAGTTGCTGGCTG
CCGGTCGCTGGTTGCTGGTACTGCTGGTGGCGTGGTTGCTGTGGCGGAAAGCGGTACGCCCGCAGCTAACACGTCGCGCC
GAGGCGGTGAAGACTGTGCAGCAACAGGCGCAGGCCCGCGAGGAAGTGGAAGATGCGGTGGAAGTCCGTCTGAGCAAAGA
CGAACAACTCCAACAACGGCGCGCTAACCAACGACTGGGGGCAGAAGTCATGAGCCAGCGTATCCGTGAAATGTCTGATA
ACGATCCGCGCGTGGTGGCGCTGGTCATTCGCCAGTGGATAAATAACGATCATGAGTAA

Upstream 100 bases:

>100_bases
CAAATAATGGGAGCGTCAATTTTTCGAGTTTGCTGACCCGGGAGTGAGTCTTGTTCCACTTTGCCAATAACGCCGTCCAT
AATCAGCCACGAGGTGCGCG

Downstream 100 bases:

>100_bases
CCTGACAGGCACCGATAAAAGCGTCATCCTGCTGATGACCATTGGCGAAGACCGGGCGGCAGAGGTGTTCAAGCACCTCT
CCCAGCGCGAAGTGCAAACC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEAS
GAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSGRDLNDAQLKYASDVEG
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPA
NNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQ
MKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDELLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
EAVKTVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE

Sequences:

>Translated_552_residues
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEAS
GAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSGRDLNDAQLKYASDVEG
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPA
NNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQ
MKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDELLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
EAVKTVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE
>Mature_552_residues
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEAS
GAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSGRDLNDAQLKYASDVEG
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPA
NNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQ
MKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDELLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
EAVKTVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=552, Percent_Identity=98.9130434782609, Blast_Score=1108, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 60515; Mature: 60515

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDG
CCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
GAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGI
HHHHHHHHHCCCCEEECCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHCCH
SQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPSLFVREQKSPSASVTVNLLPG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEECCC
RALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSGRDLNDAQLKYASDVEG
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHCCHHH
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESHAALRSRQLNESEQ
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
SGSGYPGGVPGALSNQPAPANNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCH
DRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQMKQIEALTREAMGFSEKRGD
HHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCC
SLNVVNSPFNSSDESGGALPFWQQQVFIDELLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
CCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EAVKTVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LVIRQWINNDHE
HHHHHHHCCCCC
>Mature Secondary Structure
MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAKAPDYRTLFSNLSDQDG
CCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
GAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGI
HHHHHHHHHCCCCEEECCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHCCH
SQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPSLFVREQKSPSASVTVNLLPG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEECCC
RALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLTQSNTSGRDLNDAQLKYASDVEG
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHCCHHH
RIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTEEQYRPNGDESHAALRSRQLNESEQ
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
SGSGYPGGVPGALSNQPAPANNAPISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCH
DRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGKPLPLSNEQMKQIEALTREAMGFSEKRGD
HHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCHHHCCC
SLNVVNSPFNSSDESGGALPFWQQQVFIDELLAAGRWLLVLLVAWLLWRKAVRPQLTRRA
CCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EAVKTVQQQAQAREEVEDAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LVIRQWINNDHE
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1551848; 8224881 [H]