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The map label for this gene is fliM

Identifier: 218699494

GI number: 218699494

Start: 1185646

End: 1186650

Strand: Reverse

Name: fliM

Synonym: ECIAI39_1111

Alternate gene names: 218699494

Gene position: 1186650-1185646 (Counterclockwise)

Preceding gene: 218699495

Following gene: 218699493

Centisome position: 23.12

GC content: 53.13

Gene sequence:

>1005_bases
ATGGGCGATAGTATTCTTTCTCAAGCTGAAATTGATGCGCTGTTGAATGGCGACAGCGAAGTCAAAGACGAACCGACAGC
CAGTGTTAGCGGCGAAAGTGACATTCGTCCGTACGATCCGAATACCCAACGACGGGTTGTGCGCGAACGTTTGCAGGCGC
TGGAAATCATTAATGAGCGCTTTGCTCGCCATTTTCGTATGGGGCTGTTCAACCTGCTGCGCCGTAGCCCGGATATTACC
GTCGGGGCCATCCGCATTCAGCCGTACCATGAATTTGCCCGCAACCTGCCGGTGCCGACCAACCTGAACCTTATTCATCT
GAAACCGCTGCGCGGCACAGGGCTGGTGGTGTTCTCGCCGAGTCTGGTGTTTATCGCCGTGGATAACCTGTTTGGCGGCG
ATGGACGCTTCCCGACCAAAGTGGAAGGCCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTG
GCGCTTGAAGGCTATAGCGACGCATGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAA
ATTTACCAATATCACCACCTCGCCAAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCG
AATTTAATATCTGCCTGCCATTCAGCATGATCGAGCCGCTGCGGGAATTGTTGGTTAACCCGCCGCTGGAAAACTCGCGT
AATGAAGATCAGAACTGGCGCGATAACCTGGTGCGCCAGGTGCAGCATTCACAGCTGGAACTGGTGGCCAACTTTGCCGA
TATCTCGCTACGCCTGTCGCAGATTTTAAAACTGAAACCCGGCGACGTCCTGCCGATAGAAAAACCCGATCGCATCATCG
CCCATGTTGACGGCGTCCCGGTTCTGACCAGTCAGTACGGCACTCTCAACGGTCAGTATGCGTTACGGATAGAACATTTG
ATTAACCCGATTTTAAATTCTCTGAACGAGGAACAGCCCAAATGA

Upstream 100 bases:

>100_bases
GCCGAGATTAAAACCACACTTTCCACCCCGCTTGTTGCCGGGCAACCGAAACAGGATGTCACCGACGTGCTGTATACCGC
TTTTATTCTGCGATAACGAC

Downstream 100 bases:

>100_bases
GTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAGCAAAAATCA
ACCAGCGGAAAAAGCGCTGC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINERFARHFRMGLFNLLRRSPDIT
VGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
NEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHL
INPILNSLNEEQPK

Sequences:

>Translated_334_residues
MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINERFARHFRMGLFNLLRRSPDIT
VGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
NEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHL
INPILNSLNEEQPK
>Mature_333_residues
GDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINERFARHFRMGLFNLLRRSPDITV
GAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLA
LEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSRN
EDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLI
NPILNSLNEEQPK

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=334, Percent_Identity=99.7005988023952, Blast_Score=675, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37864; Mature: 37733

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINER
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSP
HHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEEEEECCCCCEEEEECC
SLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYV
CEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
RSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HCCCEEEEEEEECCCCCEEEECCEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCC
NEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
VLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
EEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQRRVVRERLQALEIINER
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSP
HHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEEEEECCCCCEEEEECC
SLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYV
CEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
RSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HCCCEEEEEEEECCCCCEEEECCEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCC
NEDQNWRDNLVRQVQHSQLELVANFADISLRLSQILKLKPGDVLPIEKPDRIIAHVDGVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
VLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
EEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]