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The map label for this gene is fliN

Identifier: 218699493

GI number: 218699493

Start: 1185236

End: 1185649

Strand: Reverse

Name: fliN

Synonym: ECIAI39_1110

Alternate gene names: 218699493

Gene position: 1185649-1185236 (Counterclockwise)

Preceding gene: 218699494

Following gene: 218699492

Centisome position: 23.1

GC content: 56.28

Gene sequence:

>414_bases
ATGAGTGACATGAATAATCCGGCCGATGACAACAACGGCGCAATGGACGATCTGTGGGCTGAAGCGTTGAGCGAGCAAAA
ATCAACCAGCGGAAAAAGCGCTGCCGACGCGGTGTTCCAGCAATTTGGCGGTGGTGATGTCAGCGGAACGTTGCAGGATA
TCGACCTGATTATGGATATTCCGGTCAAGCTGACCGTCGAGCTGGGCCGTACGCGGATGACCATCAAAGAGCTGTTGCGT
CTGACGCAAGGGTCCGTCGTGGCGCTGGACGGTCTGGCGGGCGAACCGCTGGATATTCTGATCAACGGTTATTTAATCGC
CCAGGGCGAAGTGGTGGTTGTTGCCGATAAATATGGCGTGCGGATCACCGATATCATTACCCCGTCCGAGCGGATGCGCC
GCCTGAGCCGTTAG

Upstream 100 bases:

>100_bases
TTCTGACCAGTCAGTACGGCACTCTCAACGGTCAGTATGCGTTACGGATAGAACATTTGATTAACCCGATTTTAAATTCT
CTGAACGAGGAACAGCCCAA

Downstream 100 bases:

>100_bases
TGATGAATAACCATGCTACTGTGCAATCTTCCGCGCCGGTTTCTGCTGAGCCACTGCTGCAGGTGAGCGGCGCACTCATC
GCCATTATTGCCCTGATCCT

Product: flagellar motor switch protein FliN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGGGDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLR
LTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR

Sequences:

>Translated_137_residues
MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGGGDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLR
LTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR
>Mature_136_residues
SDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGGGDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRL
TQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=137, Percent_Identity=97.8102189781022, Blast_Score=269, Evalue=4e-74,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 14781; Mature: 14650

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGGGDVSGTLQDIDLIMDI
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECC
PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV
CEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEEEECCCEEEEECCCCE
RITDIITPSERMRRLSR
EEEEEECCHHHHHHHCC
>Mature Secondary Structure 
SDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGGGDVSGTLQDIDLIMDI
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECC
PVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVVVVADKYGV
CEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEEEEECCCEEEEECCCCE
RITDIITPSERMRRLSR
EEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]