Click here to switch to the map view.

The map label for this gene is dcm

Identifier: 218699478

GI number: 218699478

Start: 1174420

End: 1175838

Strand: Direct

Name: dcm

Synonym: ECIAI39_1095

Alternate gene names: 218699478

Gene position: 1174420-1175838 (Clockwise)

Preceding gene: 218699477

Following gene: 218699479

Centisome position: 22.88

GC content: 52.29

Gene sequence:

>1419_bases
ATGCAGGAAAATATATCAGTAACCGATTCATACAGCACCGGGAATGCCGCACAGGCAATGCTGGAGAAACTGCTGCAAAT
TTATGATGTTAAAACGCTGGTGGCGCAGCTTAATGGTGTGGGTGAGAATCACTGGAGCGCGGCAATTTTAAAACGTGCGC
TGGCGAATGACTCGGCATGGCACCGTTTAAGTGAGAAAGAGTTTGCCCATCTGCAAACGTTGTTACCCAAACCACCGGAA
CATCATCCGCATTATGCGTTTCGCTTTATCGATCTATTTGCCGGAATTGGCGGCATCCGTCGTGGTTTTGAATCGATTGG
TGGACAGTGCGTGTTTACCAGAGAATGGAACAAACATGCGGTACGCACTTATAAAGCCAACCATTATTGCGACCCGGCGA
CGCATCATTTTAATGAGGATACCCGCGACATCACCCTCAGCCATCAAGAAGGCGTGAGTGATGAGGCGGCGGCGGAACAT
ATTCGTCAACACATTCCTGAACACGATGTTTTACTGGCCGGTTTCCCTTGTCAGCCATTTTCGCTGGCTGGCGTATCGAA
AAAGAACTCGCTCGGGCGGGCGCACGGTTTTGCCTGCGATACCCAGGGCACGCTGTTCTTTGATGTGGTGCGCATTATCG
ACGCGCGTCGTCCGGCGATGTTTGTGCTCGAAAACGTCAAAAACCTGAAGAGTCACGATAAGGGAAAAACGTTCCGCATC
ATCATGCAGACGCTGGACGAACTGGGCTATGACGTTGCCGACGCAGCAGATAACGGGCCGGACGATCCGAAAATCATCGA
CGGTAAACATTTCCTGCCGCAGCACCGTGAACGCATCGTGCTGGTGGGTTTTCGTCGCGATCTGAATCTGAAAGCCGATT
TTACTCTGCGTGATATCAGCGAATGTTTCCCTGCACAGCGAGTGACGCTGGCGCAGCTGCTGGACCCGATGGTTGAGGCG
AAATATATCCTGACGCCGGTGCTGTGGAAGTACCTCTATCGATATGCGAAAAAACATCAGGCGCGCGGTAACGGCTTCGG
TTATGGAATGGTTTATCCGAACAATCCGCAAAGCGTCACCCGTACGCTGTCTGCTCGTTATTACAAAGATGGCGCGGAAA
TTTTAATCGACCGCGGCTGGGATATGGCCACGGGTGAGAAAGACTTTGACGATCCGCTGAATCAGCAACATCGTCCACGT
CGGTTAACACCTCGTGAATGCGCGCGCTTAATGGGTTTTGAAGCGCCGGGAGAAGCGAAATTCCGCATTCCGGTTTCGGA
CACTCAGGCCTATCGCCAGTTCGGTAACTCGGTGGTCGTGCCGGTCTTTGCCGCGGTGGCAAAACTGCTTGAGCCAAAAA
TCAAACAGGCGGTGGCGTTGCGTCAGCAAGAGGCACAACATGGTCGACGTTCACGATAA

Upstream 100 bases:

>100_bases
CAAGGTGATAGATGCGTTTTCACCTGCTGGCTGAGCGCGTGGCCCTAAACGGCAGTAATTATGTTAACCTGTCGGCCATC
TCAGATGGCCGGTTAAATCT

Downstream 100 bases:

>100_bases
GGCCACCCGCAGCAAAAATATGCGCGCGATTGCCACGCGTGATACGGCGATTGAGAAGCGCCTCGCCAGTTTGTTAACCG
GGCAGGGCCTTGCGTTTCGC

Product: DNA cytosine methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENHWSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPE
HHPHYAFRFIDLFAGIGGIRRGFESIGGQCVFTREWNKHAVRTYKANHYCDPATHHFNEDTRDITLSHQEGVSDEAAAEH
IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRPAMFVLENVKNLKSHDKGKTFRI
IMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHRERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEA
KYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDPLNQQHRPR
RLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIKQAVALRQQEAQHGRRSR

Sequences:

>Translated_472_residues
MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENHWSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPE
HHPHYAFRFIDLFAGIGGIRRGFESIGGQCVFTREWNKHAVRTYKANHYCDPATHHFNEDTRDITLSHQEGVSDEAAAEH
IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRPAMFVLENVKNLKSHDKGKTFRI
IMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHRERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEA
KYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDPLNQQHRPR
RLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIKQAVALRQQEAQHGRRSR
>Mature_472_residues
MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENHWSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPE
HHPHYAFRFIDLFAGIGGIRRGFESIGGQCVFTREWNKHAVRTYKANHYCDPATHHFNEDTRDITLSHQEGVSDEAAAEH
IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRPAMFVLENVKNLKSHDKGKTFRI
IMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHRERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEA
KYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDPLNQQHRPR
RLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIKQAVALRQQEAQHGRRSR

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=472, Percent_Identity=98.728813559322, Blast_Score=976, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 53522; Mature: 53522

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENHWSAAILKRALANDSAW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHH
HRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRGFESIGGQCVFTREWNKHA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHH
VRTYKANHYCDPATHHFNEDTRDITLSHQEGVSDEAAAEHIRQHIPEHDVLLAGFPCQPF
HHEECCCCCCCCHHHCCCCCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCC
SLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRPAMFVLENVKNLKSHDKGKTFRI
CCCCCCCCCCCCHHCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHH
IMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHRERIVLVGFRRDLNLKADFTLRDIS
HHHHHHHHCCCHHHCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHH
ECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPNNPQSVT
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHH
RTLSARYYKDGAEILIDRGWDMATGEKDFDDPLNQQHRPRRLTPRECARLMGFEAPGEAK
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCCCCE
FRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIKQAVALRQQEAQHGRRSR
EEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENHWSAAILKRALANDSAW
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHH
HRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRGFESIGGQCVFTREWNKHA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHH
VRTYKANHYCDPATHHFNEDTRDITLSHQEGVSDEAAAEHIRQHIPEHDVLLAGFPCQPF
HHEECCCCCCCCHHHCCCCCCCEEEEEHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCC
SLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRPAMFVLENVKNLKSHDKGKTFRI
CCCCCCCCCCCCHHCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHH
IMQTLDELGYDVADAADNGPDDPKIIDGKHFLPQHRERIVLVGFRRDLNLKADFTLRDIS
HHHHHHHHCCCHHHCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHH
ECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYRYAKKHQARGNGFGYGMVYPNNPQSVT
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHH
RTLSARYYKDGAEILIDRGWDMATGEKDFDDPLNQQHRPRRLTPRECARLMGFEAPGEAK
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCCCCE
FRIPVSDTQAYRQFGNSVVVPVFAAVAKLLEPKIKQAVALRQQEAQHGRRSR
EEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]