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The map label for this gene is insN [H]

Identifier: 218699424

GI number: 218699424

Start: 1097385

End: 1097732

Strand: Reverse

Name: insN [H]

Synonym: ECIAI39_1033

Alternate gene names: 218699424

Gene position: 1097732-1097385 (Counterclockwise)

Preceding gene: 218699425

Following gene: 218699423

Centisome position: 21.39

GC content: 49.14

Gene sequence:

>348_bases
GTGATATTCTCACCTCAACACAAAACAGGTGACTTAATGAACAAGAAAACCAAACGTACTTTCACCCCTGAGTTCAGGCT
GGAATGTGCACAGCTGATTGTTGATAAGGGCTATTCGTATCGACAAGCCAGTGAGGCGATGAATGTCGGTTCTACCACGC
TTGAGAGCTGGGTACGTCAGCTCAGGCGAGAGCGCCAAGGTATTGCGCCCTCTGCAACACCCATTACCCCTGAACAGCAA
CGTATTCGCGAGCTGGAAAAGCAGGTTCGCCGACTGGAGGAACAAAATACGATATTAAAAAAGGCTACCGCGCTCTTGAT
GTCCGACTCACTGAACGGTTTACGATAG

Upstream 100 bases:

>100_bases
TCAAAAAACTCTGTATCGATAATCCCGGACGATTATTGACCGCATAAAACCATTATCCCCCGGCTGTAGTGGCACAGTAA
ATTTGGCCACCTGATTAAAG

Downstream 100 bases:

>100_bases
TTTCCAGACTGAGTGACAGCCACTCAGTTGTCAGCCTCTGTTCCGCACTGGGAATACACCGTAGCAGTTACCGGTACTGG
CGAAAACGACGCGATACGGT

Product: transposase ORF A, IS3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 115; Mature: 115

Protein sequence:

>115_residues
MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQLRRERQGIAPSATPITPEQQ
RIRELEKQVRRLEEQNTILKKATALLMSDSLNGLR

Sequences:

>Translated_115_residues
MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQLRRERQGIAPSATPITPEQQ
RIRELEKQVRRLEEQNTILKKATALLMSDSLNGLR
>Mature_115_residues
MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQLRRERQGIAPSATPITPEQQ
RIRELEKQVRRLEEQNTILKKATALLMSDSLNGLR

Specific function: Involved in the transposition of the insertion sequence IS911 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002514 [H]

Pfam domain/function: PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 13293; Mature: 13293

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQ
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
LRRERQGIAPSATPITPEQQRIRELEKQVRRLEEQNTILKKATALLMSDSLNGLR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQ
CCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
LRRERQGIAPSATPITPEQQRIRELEKQVRRLEEQNTILKKATALLMSDSLNGLR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2163395 [H]