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The map label for this gene is rncA

Identifier: 218699304

GI number: 218699304

Start: 969567

End: 970364

Strand: Reverse

Name: rncA

Synonym: ECIAI39_0911

Alternate gene names: 218699304

Gene position: 970364-969567 (Counterclockwise)

Preceding gene: 218699310

Following gene: 218699303

Centisome position: 18.91

GC content: 51.13

Gene sequence:

>798_bases
ATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGCGCCATCTTACTTGGTGCGCTTCA
TGGCCTGGAACCGGGGCACTCAAAAACGATGATGGCGGCGTTTATCATCGCCATCAAAGGCACCATTAAACAGGCGGTGA
TGCTCGGACTGGCAGCAACTATTTCGCATACCGCAGTGGTCTGGTTAATTGCCTTTGGCGGGATGGTGATCAGCAAACGC
TTTACTGCTCAATCAGCAGAACCGTGGCTCCAGTTGATTTCCGCAGTGATCATTATTAGCACCGCGTTCTGGATGTTCTG
GCGTACCTGGCGCGGCGAACGCAACTGGCTGGAGAATATGCACGAGCACGAGCACGAGCATGATCATGAGCATCATCACC
ATCACGAACATGGCGAGTATCAGGATGCCCATGCACGAGCCCATGCCAATGACATTAAAAGACGATTTGATGGTAGAGAG
GTCACCAACTGGCAAATTTTGTTATTTGGCTTAACCGGTGGCCTTATCCCCTGCCCGGCAGCAATTACCGTGCTGTTGAT
TTGCATTCAGTTGAAAACCCTGACACTGGGCGCAACACTGGTCGTCAGTTTTAGCATTGGCCTGGCGTTAACGCTTGTCA
CCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCT
CCCTATTTTTCCAGTCTGTTGATTGGCTTAGTTGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAA

Upstream 100 bases:

>100_bases
AATATGAAAAATCATAGCACTATTAATCTACTGGGGGGCAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAAT
TAATACTAAGAATTATTATC

Downstream 100 bases:

>100_bases
CTCAATCCTTTAGGCTTGCATGTTATCTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCT
AAGGATGCTTTTAGGGCATC

Product: nickel/cobalt efflux protein RcnA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKR
FTAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENMHEHEHEHDHEHHHHHEHGEYQDAHARAHANDIKRRFDGRE
VTNWQILLFGLTGGLIPCPAAITVLLICIQLKTLTLGATLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRA
PYFSSLLIGLVGVYMGVHGFMGIMR

Sequences:

>Translated_265_residues
MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKR
FTAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENMHEHEHEHDHEHHHHHEHGEYQDAHARAHANDIKRRFDGRE
VTNWQILLFGLTGGLIPCPAAITVLLICIQLKTLTLGATLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRA
PYFSSLLIGLVGVYMGVHGFMGIMR
>Mature_264_residues
TEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRF
TAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENMHEHEHEHDHEHHHHHEHGEYQDAHARAHANDIKRRFDGREV
TNWQILLFGLTGGLIPCPAAITVLLICIQLKTLTLGATLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAP
YFSSLLIGLVGVYMGVHGFMGIMR

Specific function: Efflux system for nickel and cobalt [H]

COG id: COG2215

COG function: function code R; ABC-type uncharacterized transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NiCoT transporter (TC 2.A.52) family. RcnA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788423, Length=274, Percent_Identity=95.2554744525548, Blast_Score=474, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011541 [H]

Pfam domain/function: PF03824 NicO [H]

EC number: NA

Molecular weight: Translated: 29378; Mature: 29247

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAAT
CCHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HEHEHEHDHEHHHHHEHGEYQDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPA
HHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHH
AITVLLICIQLKTLTLGATLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHC
PYFSSLLIGLVGVYMGVHGFMGIMR
CHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIAIKGTIKQAVMLGLAAT
CHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTAFWMFWRTWRGERNWLENM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HEHEHEHDHEHHHHHEHGEYQDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPA
HHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHH
AITVLLICIQLKTLTLGATLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHC
PYFSSLLIGLVGVYMGVHGFMGIMR
CHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA